Author(s): Tsai, CH (Tsai, Chou-Huang); Chen, YC (Chen, Yu-Cheng); Chen, LD (Chen, Lieh-Der); Pan, TC (Pan, Tien-Chien); Ho, CY (Ho, Chien-Yi); Lai, MT (Lai, Ming-Tsung); Tsai, FJ (Tsai, Fuu-Jen); Chen, WC (Chen, Wen-Chi)
Title: A traditional Chinese herbal antilithic formula, Wulingsan, effectively prevents the renal deposition of calcium oxalate crystal in ethylene glycol-fed rats
Source: UROLOGICAL RESEARCH, 36 (1): 17-24 FEB 2008 Language: English
Document Type: Article
Author Keywords: calcium oxalate; ethylene glycol; traditional Chinese medicine; urinary stone; Wulingsan
KeyWords Plus: IN-VITRO; UROLITHIASIS; HYPEROXALURIA; OSTEOPONTIN;
PREVALENCE; EXPRESSION; EXTRACT; KIDNEY
Abstract: We investigated the effects of a traditional Chinese herbal formula, Wulingsan (WLS), on renal stone prevention using an ethylene glycol-induced nephrocalcinosis rat model. Forty-one male Sprague-Dawley (SD) rats were divided into four groups. Group 1 (n = 8) was the normal control; group 2 (n = 11) served as the placebo group, and received a gastric gavage of starch and 0.75% ethylene glycol (EG) as a stone inducer; group 3 received EG and a low dose of WLS (375 mg/kg); and group 4 received EG and a high dose of WLS (1,125 mg/kg). Baseline and final 24 h urine samples were collected individually; biochemical data of urine and serum were also obtained at the beginning and at the end of the experiment.
After 4 weeks, animals were killed and kidneys were harvested. The kidney specimens were examined by polarized light microscopy and the crystal deposits were evaluated by a semi- quantitative scoring method using computer software (ImageScoring). The results revealed that the rats of placebo group gained the least significant body weight; in contrast, the rats of WLS-fed groups could effectively reverse it. The placebo group exhibited lower levels of free calcium (p = 0.059) and significantly lower serum phosphorus (p = 0.015) in urine than WLS- fed rats. Histological findings of kidneys revealed tubular destruction, damage and
inflammatory reactions in the EG-water rats. The crystal deposit scores dropped significantly in the WLS groups, from 1.40 to 0.46 in the low-dose group and from 1.40 to 0.45 in the high- dose group. Overall, WLS effectively inhibited the deposition of calcium oxalate (CaOx) crystal and lowered the incidence of stones in rats (p = 0.035). In conclusion, WLS significantly reduced the severity of calcium oxalate crystal deposits in rat kidneys, indicating that Wulingsan may be an effective antilithic herbal formula.
Addresses: [Chen, Wen-Chi] China Med Univ Hosp, Dept Urol, Taichung, Taiwan; [Tsai, Chou-Huang; Chen, Yu-Cheng; Pan, Tien-Chien; Ho, Chien-Yi] China Med Univ, Coll Chinese Med, Grad Inst Chinese Med Sci, Taichung, Taiwan; [Chen, Yu-Cheng] Changhua Christian Hosp, Dept Internal Med, Changhua, Taiwan; [Chen, Lieh-Der] China Med Univ, Coll Chinese
Med, Sch Post Baccalaureate Chinese Med, Taichung, Taiwan; [Pan, Tien-Chien] Chung Shan Med Univ Hosp, Dept Integrated Chinese & Western Med, Taichung, Taiwan; [Ho, Chien-Yi] China Med Univ Hosp, Dept Family Med, Taichung, Taiwan; [Lai, Ming-Tsung]
China Med Univ, Coll Med, Dept Pathol, Taichung, Taiwan; [Tsai, Fuu-Jen] China Med Univ, Coll Chinese Med, Taichung, Taiwan; [Tsai, Fuu-Jen] Asia Univ, Taichung, Taiwan; [Chen, Wen-Chi] China Med Univ, Coll Chinese Med, Grad Inst Integrated Med, Taichung, Taiwan Reprint Address: Chen, WC, China Med Univ Hosp, Dept Urol, No 91 Hsueh-Shih Rd, Taichung, Taiwan.
E-mail Address: [email protected]
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Publisher: SPRINGER
Publisher Address: 233 SPRING STREET, NEW YORK, NY 10013 USA ISSN: 0300-5623
DOI: 10.1007/s00240-007-0122-4 29-char Source Abbrev.: UROL RES ISO Source Abbrev.: Urol. Res.
Source Item Page Count: 8
Subject Category: Urology & Nephrology ISI Document Delivery No.: 258OM
