苯丙胺酸與丙胺酸添加對白殭菌素產量影響之探討柯培元、張耀南

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苯丙胺酸與丙胺酸添加對白殭菌素產量影響之探討柯培元、張耀南

E-mail: 9509700@mail.dyu.edu.tw

摘要

本研究以搖瓶培養方式進行，以苯丙胺酸–β-丙胺酸添加的二次交叉試驗法探討本土白殭菌(Beauveria bassiana A1)生合成白殭菌素(beauvericin; BEA)產量所需之最適胺基酸添加組合及其最適濃度之試驗。先前回應曲面法試驗的結果顯示，當以25.0 g/L 葡萄糖、10.0 g/L NZM broth、5.0 mL/L玉米萃取液及2.0 g/L磷酸氫二鉀為培養基質時，且此基質初始酸鹼值為pH 5.7，在26℃溫度下，以150 rpm振盪培養六天後，白殭菌BEA產量為1.34 mg/L；若上述培養基質經苯丙胺酸-丙胺酸添加的二次交叉試驗法探討後，實驗結果發現，唯有單獨添加0.2 g/L苯丙胺酸於培養基質時，其BEA產量可提高

至2.74~2.99 mg/L。然而，β-丙胺酸添加可能對白殭菌BEA產量有抑制作用。

關鍵詞 : 白殭菌、白殭菌素、苯丙胺酸、β-丙胺酸

目錄封面內頁簽名頁授權書 iii 中文摘要 iv 英文摘要 v 誌謝 vi 目錄 vii 圖目錄 x 表目錄 xi 第一章前言 1 第二章文獻回顧 4 2.1 微生物農藥 4 2.2 蟲生病原真菌的簡介 5 2.3 白殭菌 7 2.4 白殭菌素 14 2.5 回應曲面實驗設計法 19 2.5.1 二水準因子設計 21 2.5.2 陡升路徑法 26 2.5.3 中心混成設計 27 2.6 回應曲面模式適切性之統計檢驗 27 2.7 因子影響效應之分析 29 第三章材料與方法 31 3.1實驗材料及方法 31 3.1.1實驗材料 31 3.1.2儀器設備 32 3.2菌種培養方法 33 3.3白殭菌素萃取方法 34 3.4白殭菌素之定量分析 34 3.5回應曲面法之實驗設計 35 3.5.1一階因子設計實驗 36 3.5.2陡升路徑法 36 3.5.3中心混成實驗設計 37 3.3.4回應曲面模式適切性之統計檢驗 37 3.6苯丙胺酸-丙胺酸交叉試驗之最適添加濃度探討 38 3.6.1苯丙胺酸-丙胺酸添加之二次交叉試驗法 38 3.6.2胺基酸添加之濃度梯度試驗 43 3.6.3最適胺基酸添加濃度之培養天數探討 44 第四章結果與討論 47 4.1回應曲面法實驗設計之結果與討論 47 4.1.1 一階因子設計實驗 47 4.1.2 陡升路徑實驗 49 4.1.3 中心混成設計實驗 49 4.1.4 回應曲面模式適切性之統計檢驗 53 4.2苯丙胺酸-丙胺酸交叉試驗之最適添加濃度探討 61 4.2.1 苯丙胺酸-丙胺酸添加之二次交叉試驗法 61 4.2.2 胺基酸添加之濃度梯度試驗 63 4.2.3 最適胺基酸添加濃度之培養天數探討 66 第五章結論 70 參考文獻 72 附錄 87 圖目錄圖2.1 白殭菌素與恩鐮孢菌素之化學結構 16 圖2.2 回應曲面圖 23 圖2.3 回應曲面進行步驟流程圖 24 圖2.4 回應曲面法實驗設計之概念圖 25 圖2.5 回應曲面法之陡升路徑圖 28 圖4.1 玉米萃取液與NZM broth對BEA產量之回應曲面圖 55 圖4.2 玉米萃取液與磷酸氫二鉀對BEA產量之回應曲面圖 56 圖4.3 玉米萃取液與葡萄糖對BEA產量之回應曲面圖.. 57 圖4.4 磷酸氫二鉀與NZM broth對BEA產量之回應曲面圖 58 圖4.5 葡萄糖與NZM broth對BEA產量之回應曲面圖 59 圖4.6 磷酸氫二鉀與葡萄糖對BEA產量之回應曲面圖 60 圖4.7 第一次苯丙胺酸-丙胺酸添加交叉試驗對白殭菌素產量之影響 65 圖4.8 第二次苯丙胺酸-丙胺酸添加交叉試驗對白殭菌素產量之影響 66 圖4.9 苯丙胺酸濃度梯度試驗對白殭菌素產量之影響.. 68 圖4.10 苯丙胺酸(0.02%)培養天數探討對白殭菌素產量之影響.. 69 表目錄表2.1目前發展中或已發展之真菌殺蟲劑 7 表2.2具有生物活性化合物製成之藥物 9 表3.1 24完整因子實驗設計之變數及層階 39 表3.2陡升路徑實驗設計 40 表3.3中心混成實驗設計表 41 表3.3中心混成實驗設計表(續) 42 表3.4苯丙胺酸-丙胺酸添加之第一次交叉試驗設計表 45 表3.5苯丙胺酸-丙胺酸添加之第二次交叉試驗設計表 46 表4.1一階因子設計實驗之結果 48 表4.2陡升路徑實驗設計之結果 50 表4.3中心混成實驗設計之結果 51 表4.4四種因子中心混成實驗之迴歸分析表 52 表4.5回應曲面模式之變異數分析表 54 表4.6回應曲面模式中心預估測試點實驗值 62

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苯丙胺酸與丙胺酸添加對白殭菌素產量影響之探討 柯培元、張耀南