原文題目

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口腔病理科 On-Line KMU Student Bulletin

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原文題目(出處)： Immunohistochemical detection of laminin-1 and Ki-67 in radicular cysts and keratocystic odontogenic tumors. BMC Clinical Pathology 2011,11:4.

原文作者姓名： Mohamed S Ayoub, Houry M Baghdadi, Moataz El-Kholy 通訊作者學校： Oral Pathology Department, Faculty of Dentistry, Ain Shams

University, Cairo, Egypt 報告者姓名(組別)：陸弘志 Intern E組

報告日期： 101/1/16

內文：

Background

1. Radicular cysts (RCs) are a direct sequel to chronic apical periodontitis- epithelial rests of Malassez.

2. Keratocystic odontogenic tumor (KCOT), known as odontogenic keratocyst (OKC ),a developmental odontogenic cyst from the dental lamina remnants .

3. Expression of laminin-1 in normal oral mucosa , odontogenic cysts and odontogenic tumors was examined in several studies.

A. Sections of normal oral mucosa and odontogenic cysts stained for laminin-1 showed a distinct linear deposit of strong intensity at the basement membrane junction but not in the cytoplasm of the epithelial cells.

B. Sections of odontogenic tumors stained for laminin-1 showed strong reactivity at the basement membrane junction as well as in the cytoplasm of all tumor cells.

4. The expression of laminin-1 in the cytoplasm of the tumor cells, but not in the normal mucosa may be a useful marker to distinguish these two types o f epithelium and it may suggest that laminin-1 influences the proliferation activity toward tumor potential .

5. Ki- 67 antigen is the prototypic cell cycle related nuclear protein, expressed b y proliferating cells in all phases of the active cell cycle (G1, S, G2 and M phase) and reaches a peak in the G2 and M phases.

6. It rapidly degrades after mitosis .

7. Ki-67 antibodies are useful in establishing the cel l growing fraction in neoplasms.

Method

1. Specimen selection

25 formalin-fixed ,paraffin-embedded tissue blocks of odontogenic cysts .

13 cases were diagnosed as radicular cysts (RCs) and 12 cases were diagnosed as keratocystic odontogenic tumors (KCOTs) .

2. Immunohistochemical procedures All specimens is 4μm sections

A. Cut and mounted on positively charged glass slides.

B. Deparaf-finized with xylene and rehydrated in graded ethyl alcohol C. Immersed in citrate buffer solution of pH 4.8

D. Put in the microwave oven before staining procedures.

E. For immune staining a universal kit ( R&D Systems;USA) was used Results

I. Immunohistochemical Results

A. 10/13 RCs were immunopositive to laminin-1 representing 76.9% .

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B. 1/12 KCOTs revealed immunopositive reaction to laminin-1 representing 8.3% . C. 7/13 RCs were immunopositive to Ki-67 representing about 53.8 % where there

was severe inflammation in the connective tissue.

D. 12/12 KCOTs were positive for Ki-67. The reaction was nuclear and confined to the basal and suprabasal cells of the epithelial lining .

Discussion

1. Negative immunostaining for laminin-1 was observed in 3 cases of RCs ( 23. 1%) that showed severe inflammatory reaction in the connective tissue wall of cysts.

 Furuyama said ability of epithelial cells to form continuous basement

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membrane was lost in the presence of inflammatory cytokines.

2. Negative immunoexpression of laminin-1 in 11/12 cases of OKCs, was concluded that laminin-1 was expressed in RCs, dentigerous cysts and odonto-genic keratocyst with different distribution patterns and intensity.

 Gurgel e t al,said 20/20 cases of OKS expressed laminin-1 in areas of acanthosis, subepithelial split and epithelial buds.

3. 7/13 of RCs included in the present study were immunopositive for Ki-67, which represent 53.8% of the total cases.

 Willoughby et al. concluded that mild inflammatory injury stimulates epithelial proliferation, whereas more sever e inflammation depresses it, perhaps due to more extensive progenitor-cell damage

Conclusions

1. The benign nature o f radicular cysts and the aggressive behavior of keratocystic odontogenic tumors could be explained by the expression of laminin and Ki-67.

2. Laminin-1 and Ki-67 could be valuable markers for the prediction of the biologic behavior of cystic lesions.

題號題目

1 Which is a component of the nevoid basal cell carcinoma syndrome?

(A)KCOT(keratocystic odontogenic tumor) (B)COC(Calicifying odontogenic cyst) (C)AOT(Adenomatoid odontogenic tumor) (D)SCC(Squamous cell carcinoma )

答案(A) 出處：Oral & Maxillofacial Pathology 3^rd ED P.688

題號題目

2 下列何種顎骨囊腫最容易變成Ameloblastoma ,所以治療時比較傾向以

積極之手術來治療?

(A)Dentigerous Cyst (B)Radicular cyst (C)Residual cyst (D)Primordial cyst

答案(D ) 出處：Oral & Maxillofacial Pathology 3^rd ED P.683-6.