原文題目

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口腔病理科 On-Line KMU Student Bulletin

原文題目(出處)： Role of human papilloma virus-16 in the pathogenesis of oral lichen planus. Int Dent J 2015;65:11-4

原文作者姓名： Chetan A. Pol, Suvarna K. Ghige and Suchitra R. Gosavi 通訊作者學校： Department of Oral Pathology & Microbiology, Government

Dental College & Hospital, Nagpur, Maharashtra, India 報告者姓名(組別)：王盛弘 Intern J 組

報告日期： 103/5/14

內文：

 Introduction

- OLP is a chronic immunological inflammatory mucocutaneous disease - affects 0.1% to about 4% of the population

- Common in middle-aged person and women

- Buccal mucosa, tongue and gingiva are commonly affected

- Often bilateral. Presentation with reticular, papular, plaque-like, atrophic and ulcerative.

- Immunologically hypersensitivity reaction. Intense band-like T-cell infiltrate in the epithelium–connective tissue

- Viruses (such as HPV HHV) found play a role in the pathogenesis of OLP.

Viruses alter host cell function by the expression of cellular proteins.

- Aim of the present study

- Prevalence of OLP& HPV-16 and to evaluate whether any clinical features correlate with this virus.

 Materials and methods

- The research was approved by Maharashtra University of Health Sciences (MUHS)

- retrospective study performed on lesional and normal tissue embedded in preserved wax block

- 30 patients with OLP (with minimum age 21 years and maximum age 58 years) - The patients were classified according to age, gender and localisation of the lesion - All cases of OLP were of the reticular form and patients had no history of

smoking, alcohol consumption or betel-nut chewing habit

- Thirty normal oral mucosal tissue specimens, free of inflammation and necrosis (from subjects with a minimum age of 18 years and a maximum age of 52 years) - Biopsy samples were processed using the poly-HRP method

(Deparaffinisation and rehydration -3% hydrogen peroxide for 5 minutes.

Antigen-retrieval solution (citrate buffer, pH 6.0) in a microwave for 15 minutes.

Incubated with HPV-16 primary antibody at room temperature for 30 min.

Then slides were incubated with poly-HRP secondary antibody for 30 minutes.

Incubated with DAB HRP substrate)

- brown nuclear staining was accepted as positive staining for HPV-16 antibody.

- Statistical analyses were performed using Fisher’s exact test.

 Results

- Twenty-one (70%) of 30 subjects with OLP were HPV-16 positive, all subjects in the control group were negative for HPV-16

- The P value obtained from Fisher’s exact test showed a significant relationship (P = 0.0001) between HPV-16 infection and OLP

- no statistically significant correlations between age, gender, localisation of the lesion and HPV-16 positivity

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 Discussion

- OLP is a chronic inflammatory disease which involves cell-mediated immune dysregulation.

- Potentially malignant lesion of the oral mucosa about 0-6.25%

- HPV in premalignant lesions has also been studied, infection upper respiratory tract may play a role in the head and neck tumor

- HPV-16, associated with potentially malignant lesions and with oral squamous cell carcinoma.

- High-risk HPV-16 produces two oncoproteins, E6, E7.

- E6 protein diminishing the ability of the cell to undergo apoptosis.

- HPV E7 protein binds the retinoblastoma protein (pRb), resulting in loss of cell-cycle control.

- Keratinised tissue is more resistant to HPV infection

- HPV-16 was found in 85% of nonkeratinised tissues and in 15% of keratinised tissues.

- P-value of Fisher’s exact test showed a statistically significant relationship between PV-16 positivity and non-keratinised tissue.

 Conclusion

- HPV infections may play an important role in the pathogenesis of OLP

- Due to oncogenic potential of HPV-16, patients with OLP should be screened for the presence of this virus, and adequate long-term follow-up should be done

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