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ԃ 7 Д 11
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ty
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מೌᏢس
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3.2 allel ng metho
س
٢܊
les, ods
ૈֹԋ೭ҽ ࢲᆶፐ
ᓉە! റγޑ Ўளа׳уֹ
གᖴسᒤϦ
܍ᆶϩ٦
ࢂᒧࣴز ӧ҂ٰں๏
གᖴჴᡍ࠻
գॺӧჴᡍ ሶӭޑྃДࢂ
ѺǶགᖴ གᖴϩғࣴ
നࡕǴགᖴ
ीၗБय़ϐ
ୖуКᖻޑუ
ҽፕЎǴ၈
ϐᜢЈǴ ޑεΚڐշ
ֹǶ Ϧ࠻ࠧᏢ ǶऩࢂؒԖ ز܌Ƕίق
๏ޗǶ
࠻ޑ܃ඁǴ ᡍᆶғࢲ
ࢂךޑᄪ۩
ᖴၭޑᎄ
ࣴᆶౢࠔу ᖴৎΓॺܭ ϐڐշϩ
უՔ୯҉ǵ
၈ኑགᖴࡰᏤ ҭ٬Ꮲғ७ շǴٿՏԴৣ
ᏢۆᆶՏػᏢ ԖࠧᏢۆޑ قᇟΨ߄ၲ
ྷଈǴ܃⣬
ࢲޑڐշ٬
۩ǶќѦձ ᎄՏܴӃғᆶ уπ࠻ܭሺᏔ ܭᅺγය໔
Ƕགᖴ㧦Ϭ εݞǵӹ
ठᖴ
Ꮴ௲Цٵ ७གྕཪǶ
ৣܭα၂ය
ᏢߏޑऐЈ ޑڐշǴΨ
ၲόΑ೭ҽ
⣬ǴޱျᏢ
٬ჴᡍ࠻ԋ ձགᖴဃᝬ ᆶख़࠶Ꮲߏ ᏔБय़ޑЍ ໔ӧғࢲ
Ϭ೭٤Вη ӹаϷٵী
ٵ റγޑ ќѦགᖴα ය໔ޑࡰ҅ᆶ
ЈᆶගឫǴа Ψך፯
ҽགᖴǴᜫԾ
ᏢߏǴٵሺ ԋࣁঁऍӳ ᝬޑڐշǴࡐ ߏǴаϷၭ
Ѝජᆶڐշ
ޑڐշᆶх ηٰޑЍᆶ
ীǴᡣჴᡍϐ
ޑЈ௲Ꮴᆶ α၂ہ
ᆶፕЎঅׯ
аϷ݅ৱҸ
ֹӄόӕ ԾρΨૈа
Ǵ܃ᑉǴҲ ӳޑӦБǴ ࡐଯᑫךॺ
ޑמπܭ Ƕ
хǴΨ
ᆶഉՔǴΨ ϐᎩϝԖॣ
ᆶࡰ҅ǶԴ
၃ӕ! റγ ׯޑගᗺࣣ٬
Ҹ! റγ๏ϒ ӕޑΓғၰၡ аӕኬคدޑ
ҲಡǴߘ⣶
ૈᆶգॺو ॺૈӧനࡕᜢ ܭኬҁ௦ϐ
ձགᖴް
Ψགᖴ೭൳ԃ
ॣޑኃᙖ Դৣܭ
γᆶ
٬ҁ
ϒ
ၡǴ ޑЈ
Ǵྷ
وၸ ᜢᓐ ϐڐ
ܭ ԃ
Ƕ
ᒵ………
ԛ………
ԛ………
Ўᄔा………
Ўᄔा………
ق………
Ўᔠ…
ǵ٢ҔФ Βǵ٢܊ݹ Οǵғ٢ኬ ѤǵФଫЬ
ᆶБݤ
่݀ᆶፕ
่ፕ………
ୖԵЎ…
………
………
………
………
………
………
………
Фϐᅿᜪ…
ݹϐϟಏ…
ኬࠔճҔϐ Ьाಔᙃ࣬
ݤ…………
ፕ…………
………
………
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ϐϟಏ………
࣬ፄӝᡏ୷
………
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Ҟᒵ
………
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୷Ӣϐࣴز
………
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।ԛ
…… 1
…… 2
…… 3
…… 4
…… 6
…… 8
… 10
… 10
… 15
… 25
… 31
… 37
… 45
… 69
… 70
1. தـϐ٢ 2. аݤ 3. λႵᆶΓ 4. ၂ᡍी
5. Ծғ٢ኬ 6. PCR ౢ 7. ᡏಒझኧ
. ٿᅿόӕ
٢ҔФଫࠔ ݤܜڗФ٢ Γᜪ MHC
ीࢬำკ…
ኬҁܜڗ୷ ނપϯϐࢬ ኧȐSCCȑ
Ҕಔ D
ࠔᅿ………
DNA ϐࢬ
୷Ӣ………
………
Ӣಔ DNA ࢬำკ……
ȑᆶ٢ಔԋ DNAϐႝݚ
კԛ
………
ࢬำკ……
………
………
A ࢬำკ…
………
ԋϩϐᜢ߯
ݚ่݀Кၨ
………
………
………
………
………
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߯…………
ၨ…………
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।ԛ
… 14
… 29
… 32
… 38
… 42
… 43
… 51
… 57
1. ऍ୯ी
2. Ф٢ᡏಒ 3. ճҔϤᅿ 4. ܰ௵ག܄
5. ٢܊ݹᆶ 6. ҂วݹය . ٢ໆᆶ٢ 8. ٢ໆᘜ 9. ٿᅿόӕ 10. Ѡεၭ
1. Ѡεၭ
2. ל٢܊ݹ 3. ܄ဂ 4. ܰ௵ག܄
5. όӕҬඹ
ीԃᓐ ಒझኧ֖ໆ ᅿόӕБݤ
܄ǵ܄
ᆶғ٢ࠔ፦
ය໔ᆶวݹ ٢ಔԋϩϐ P
ᘜኳࠠ……
ӕҔಔ
ݜ٢Фϐ
ϐើФ ݹဂಔϐҬ ဂಔϐҬඹ
܄ဂಔϐҬ ඹ୷Ӣࠠᆶ
Ӣ٢܊ݹϐ ᆶ٢ౢໆཞ ԾᆟԲ٢ύ
܄аϷל٢ ϐᜢ߯……
ය໔ϐ٢ໆ Pearson࣬ᜢ
………
ಔܜڗ DNA ϐ DRB3.2 Ф DRB3.2 ୷ Ҭඹ୷Ӣࠠᓎ ඹ୷Ӣࠠᓎ
Ҭඹ୷Ӣࠠᓎ ᆶ٢ಔԋϩϐ
߄ԛ
ϐཞѨ……
ཞѨԭϩК ύܜڗ DNA
܊ݹဂಔϐ
………
ໆᆶ٢ಔԋ ᜢંତ……
………
A ϐКၨ…
Ҭඹ୷Ӣ
୷Ӣࠠᓎ
ᓎ………
…………
ᓎ………
ϐᜢ߯……
………
ᜢ߯………
A ϐКၨ…
ϐ٢ໆᆶ٢
………
ϩКၨ……
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ࠠᓎ……
ᆶВҁϷу
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Кၨ…………
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।ԛ
… 19
… 22
… 30
… 46
… 47
… 48
… 54
… 55
… 57
… 60
…. 61
… 63
… 64
… 64
… 68
٢ౢໆᆶғ ӭъ۹ౣӵ Ъख़ाϐ੯ੰ
ϐᛌᙴৣ
ޣёૈԋ٢
ᑔᒧǴଞ ѨፐᚒǶ
Ьाಔᙃ࣬
৲৲࣬ᜢǶФ gen, BoLA
੯ੰխࣝᆶ RB3ȑς
ёૈᆶФଫխ ϐғ٢ኬҁڗ Չ ࣴ ز ϐ ё
rovementȑ ϐᜢ߯Չ ΟঁဂಔǴϩ ݹဂಔȐ40%
ҁ၂ᡍ่݀
A٠Չ P ག܄ဂಔԖ 4
2703Ҭඹ୷
՟ϐ่݀
ғ٢ಔԋϩ ӵלੰ܄
ੰǴѿФ
Ҕǵᛰނ ٢ФගԐి
ଞჹԜᜪ୷
࣬ፄӝᡏ ФଫЬाಔ
ȑǴҗΟঁό ᆶғౢ܄ރ
วڀԖ࣬
խࣝ܄ރ࣬
ڗжϐ ё ૈ ܄ Ƕ ၂
ၗаଞჹ ՉፕǶФ ϩձࣁܰ௵
%аΠȑǶ
݀ᡉҢܭ 2 CR-SBTϩ 4ᓐǶܭԜ 1
୷Ӣࠠـܭ ǴᡉҢځࣁ
ϩϐගϲதࣁ
ख़ाϐ٢Ф Фଫགࢉ٢܊
ނǴаϷቲక
ి؎Ƕ߈ԃϐ
୷Ӣޑ
ᡏȐmajor hi ᙃ࣬ፄӝ όӕୱಔ ރϐᚒύቶ
࣬ଯࡋϐ
࣬ᜢǶҁ၂ᡍ ϐՈనᆶಔᙃ ၂ ᡍ ය ໔ ӕ
ჹᡏಒझኧ Фဂ٩ځܭ၂
௵ག܄ဂಔ
3 ᓐ၂ᡍФ ϩǹځύל
7ᓐঁᡏύ ܭל٢܊ݹ
ؼӳϐל
ᄔा!
ࣁݜ٢Фϐ Ф଼ந܄ރ
܊ݹǴό
కϐғ٢
ϐࣴزว
ޑࣴز܈ё
stocompatib ӝᡏᆀࣁ ಔԋЪᆶלচ
ቶݱӦࣴ
ϐӭᄊ܄ǴЀ ᡍԏٰԾ
ᙃኬҁǴа ӕ ਔ ԏ ٢ ኧȐsomatic c ၂ᡍය໔ᡏ Ȑ100%ȑǵ
ФଫύǴԖ ל٢܊ݹဂ ύӅว 11
ݹᆶ܄
ל٢܊ݹҬ
ϐЬाػᅿ ރǶ!ᖐٯԶ
ᏤठΓΚ
ӢનᏤठ
ࢌ٤੯ੰ
ёှ،Ӣ੯
bility comp ФଫరЃౚ চևԖᜢ
ࣴزǶBoLA ЀаಃΒঁ
ѠεᏢ၂ аճҔ ٢ Ф ဂ ܄ ૈ
cell countsȑ ᡏಒझኧၲ
܄ဂಔ
17 ᓐঁᡏ ဂಔԖ 6 ᓐ
ঁόӕϐ D ဂಔύǴᆶ
ඹ୷ӢࠠǶ
ҞǴฅԶ ԶقǴ٢܊ݹ
ԋҁቚу ቚуᚐѦϐ ϐל܄܈ё
ੰԶԋϐ
lexȑᆶথ٢ ౚלচȐbov ᜢǹӢԜǴB
୷Ӣ০ಃ
ঁѦᡉηȐe ၂ᡍၭ 23
ߚߟΕԄ௦
ૈ ׯ ी ฝ ȑᆶғ٢ಔ
ᖏ٢܊ݹ ಔȐ40 Կ 80
ᡏёԋфԾ٢ Ǵ܄ဂ DRB3.2Ҭඹ ᆶу৾εܿ۞
Ƕ*1501 Ҭඹ
Զߏයᒧܘ ݹࣁݜ٢Ф ǴЪӢܭݯ ϐғౢԋҁ ёᙖҗխࣝ
ϐ٢ໆᆶಔ
٢ނϐխ vine lymph BoLA୷Ӣ
ಃΟঁ DR exon 2ȑϐ
3ᓐើФ ௦ኬܭݜ٢ ฝ Ȑ Dairy ಔԋϩᆶ DR
ݹભϐᓎ 0%ȑǴаϷ
٢ࠔኬҁύ ဂಔԖ 7 ᓐ ඹ୷ӢࠠǴ*
۞ϐើФ ඹ୷Ӣࠠϐ
ܘޑၸ Фനத ݯᕍၸ ҁǴ׳
ࣝᆶᒪ ಔԋϩ
խࣝس hocyte Ӣςܭ
ୱ ϐӭᄊ Фݜ٢ ٢Фଫ Herd RB3.2 ᓎϩ Ϸל٢
ύܜڗ ᓐǴܰ
*1201 Фࣴز ϐપӝ
ـܭܰ௵ག Ң၀Ҭඹ୷Ӣ
ᗖӷǺФଫర
ག܄ဂಔύ Ӣࠠᆶ٢܊
రЃౚלচ
ύǴЪԖԜ ݹϐ௵ག܄
চǴDRB3.2
!
Ԝપӝηϐ
܄࣬ᜢǴࡌ
Ǵ٢܊ݹǴ
ϐঁᡏځว ࡌВࡕёӈ
Ǵ٢ФǶ
ݹำࡋ֡ଯ ӈࣁػᅿᒧ
ଯܭځдФ ᒧܘϐୖԵ
ФଫǴ Ƕ
Increasing n breeding
tance have d-wide dis nditures of arding milk
be genetica nomical los
umers. Con ied expectin
The major mmalian im phocyte anti antigens pre
es as well morphic, an y, we collec actions from preliminary a from Dair stigate the r B3.2 alleles.
led the cow mal (40 to 80
productivit goal for d been ignore ease in da f labors, vet k, and cullin
ally determ ses and in nsequently, ng to be use r histocom mmune syst igen (BoLA
sentation. H as produc nd exon 2 (
cted milk s m 23 dairy H
feasibility o ry Herd Imp relationship . According ws into thr
0%), and se
ty such as dairy cattle
ed for a lon iry herds a t service, dr ng of cows mined. Selec crease milk
genetic a d in disease mpatibility c tem. For d A) complex,
Hence, BoL ction traits.
(DRB3.2) m samples ins Holstein cow
of construct provement p between s
g to the fre ree classific ensitive one
Abstract
milk yields e. However ng time. For
and leads t rugs, but al s. Nowaday cting resista k quality, w and immun e resistance complex (M dairy cattle , consisting LA genes ha . The DRB may potenti stead of blo ws in NTU ting noninv
(DHI) in re somatic cell equency of cations, kno
(100%).
t
s and milk r, some hea r example, m to not only lso the loss ys, it is evid
nce to heal which is im nological m
selections.
MHC) grou e, MHC r
of three cl ave been ex B3 locus o
ially affect ood or tissu Experimen asive sampl ecently five l counts (SC
that SCC r own as res
componen alth traits mastitis is a y add cost ses from low
dent that di th problem mportant to markers hav
ups are cl referred to
asses and b amined for of BoLA c immunity ue as the re ntal Farm, tr ling method e months w
CC), milk c eached clin sistant grou
nts is alway such as di an importan ts, includin
wer produc isease resis ms can reduc o ranchman ve been w
losely linke as the b being respon
various im class II is
in cattle. In esource of rying to eva ds in dairy h were also us
components nical mastit up (under 4
ys the isease nt and g the ction ,
stance ce the n and widely
ed to ovine nsible mune very n this
DNA aluate herds.
sed to s, and is we 40%),
The result essfully in ormal, and e found. All not in sensi allele *15 itive group, e of mastitis
words: BoL
ts showed PCR-SBT a four in sen leles *1201 itive one, an 501 homozy , which ma s in breedin
LA, DRB3.2
17 individ analysis, inc nsitive, resp
and *2703 nd this resu ygous expr ay tend tow ng.
2, mastitis, c
duals from cluding six pectively. A 3 were only
ult is in acc ressed mor ward inflamm
cows.
23 dairy individuals Among thes y found in r cordance wi re serious m
mation easi
cows could s in resistan e 17 indivi esistance an ith former s mastitis lev er. It may b
d be seque nce group, iduals, 11 a
nd normal g study. Indiv vel and on be the cand
enced seven alleles group vidual nly in
didate
Ѡ٢ϐ 2ԃ 1 Д 1
ϣႀၭ٣
ᆶऍ୯εໆ٬
ԋᕇճޜ໔ ஏᅱғ٢ࠔ Οε੯ੰȐഋ ԋҁǶ٢܊ݹ చҹϐǴӢ ݹϐวғջࣁ ߈ԃٰᒿ
FᢀۺǴջ m discomfort ܭ৮ឨϷᓸΚ
eedom to ex 1ДϦѲΑ ౦ǴаϷᆅ
ࢂჹΓᜪᙴᏢ ᡍϐௗڙࡋஒ х֖ჹӚ၂ᡍ
٬Ҕނ
ኧҞࣁϐ धϐԵໆΠ
ϐวςҗ В҅ԄуΕ
य़ᖏ׳ࣁ
٬Ҕҏԯғ ໔ϐཞѨǶ ࠔ፦Ǵۈૈ
ഋǴ2002ȑ ݹϐวғҭ ӢԜӧటӕ ࣁ࣬ख़ा
ނᅽճ խܭទȐ tȑǵխܭ
ΚȐfreedom xpress norm
ჴᡍނࡰ
ნϣҺՖ ᏢཱུԖଅ
ஒவ 83 फ़Կ ᡍނᆶᚲ
ՉࣽᏢᔈ Ǵ٠а٬
Π܌ुۓϐ
җԐයλೕ
ΕШࣚຩܰ
ࣁᚳεϐѱ
ғౢඹжૈྍ
ӧ୯ϣ٢ౢ
ૈᆢۓϐ Ǵନ٢ໆϐ ҭᏤठғ٢ ӕਔᆅғౢ
ाϐፐᚒǶ ճᆶΓၰཀ
Ȑfreedom fr
্ǵภधᆶ m from fea mal behavio
ࡰзঅ҅ࡰ
Ֆނჴᡍϐ
ϐ၂ᡍǴ
Կ 65%ǶѠ ᚲނՉᏹբ
ᔈҔǴᔈᏃໆ
ނౢғനϿ ϐǶ
൘ǵق
ೕኳϐᔼ ಔᙃȐWor
ᝡݾǶ20
ྍϐݩΠ
ౢໆදၹό ϐᝡݾΚǶ ϐཞѨϐѦ
٢ύᡏಒझ
ౢԋҁᆶғ
ܩᓐǴԐ from hunger ᆶ੯ੰȐfr ar and distre orȑǶࣁၲԋ
ࡰзගਢǴ׆
ϐՉǹऍ
ѿ၂ᡍ
Ѡܭ 2010 բႩᎦೕጄ ໆᗉխ٬Ҕ ϿภधϷ
ق!
ኳԄᙯࣁҾ rld Trade Or 006 ԃǴӧ ΠǴठ٬ႀ όىϐݩ Ƕ٢܊ݹǵ Ǵۘԋ
झኧቚуǴ ғ٢ࠔ፦ϐ
Ԑܭ 1960 ԃ r and thirstȑ
eedom from essȑǴаϷ ԋமϯނᅽ
׆ఈૈᕭ ऍ୯ނߥ
ނᎁڙੵ
0ԃ 1 Д 27 ጄᆶݤచѦ
Ҕࢲᡏނ
্ϐБԄࣁ
Ҿϯࠠᄊ rganization, ӧӕਔڙډ୯
ၭႩԋҁ ΠǴԖफ़ ᕷምᛖϷ
ӭݯᕍϐ Զᡏಒझኧ
ݩΠǴӵ
ԃжߡςගр ȑǵխܭғ
m pain, inju Ϸ߄҅தՉ
ᅽճϐҞ
ᕭ෧Ӛ୯ܭ
ៈڐϐፓ ภ܈ԝΫ 7Вঅ҅ނ ǴځύಃΟ
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ၸཕ٢ سϐٛᑇ 8Ȑinterleu วݹՏᆫ Ȑmacrophag ಔ ᙃ ࣬ ፄ ౚаЇวᆶ
ᆶᐱҥ٢ Ǵࡕϩ܊
Ԗϩݜфૈ
่ጌಔᙃϷ 50 mϐౚ lumenȑǵ٢ ගٮݜ٢ಒ
canalȑǶ٢ etteȑǴЬा
๚ᆶѦࣚϐ
߯ࡰ٢ གࢉࣁЬǶ ٢ഢࢉ
ᑇᐒᙯԶЇ ukin-8, IL-8 ᆫǶѿ geȑൂਡ ፄ ӝ ᡏ Ȑ m
ۓלচ࣬
٢ဏǴ
܊߾
ૈǴ٢ Ϸિެ
ౚރ٢ ٢ᆅǵ ಒझӝ
٢ᓐ๚
ाфૈ
ϐ
٢ဏӢ
༾ғ
ࢉ๏ځ วݹ 8ȑϷ ѿݹ܄
ਡౚǴ major
࣬ᜢϐ
Ϻխࣝϸᔈ ҊϐᔼᎦǴӢ
ݹϐФଫғ қՈౚΕ ӧѠ٢ actiaeȑǵߎ ዀȐMycop 2ȑǶऩа٢
clinical mas Ᏽ߈ԃଞჹѠ
ੱȐketosisȑ ౦ՏȐdispla
നଯϐ੯ੰ
ค٢ౚ
! ค٢
Фްǵཕ٢ ϟǴ٠ܭᔒ ౚߡёِ
ޑ୴ᑈу
ԋᝄख़ϐ 2003ȑ
ȐBenjamin ӢԜܭ٢܊
ғ٢ύࣣё Εғ٢ύ܌
Фဂύ ߎဟౚ
plasma spp
٢܊วݹำࡋ stitisȑᆶߚ Ѡᛌᙴৣ
18.1%ǵബ acement of a
ੰȐഋǴ
ȐStretococ
ౚࣁ࣬
٢ᐒᏔ܈ᏹբ ᔒ٢ਔࢉԿ
ِೲጭۯག уமݹ܄ϸᔈ ϐ੯ੰǴՠ٢
ni et al., 200
܊วݹਔՈన ёෳளၨଯϐ
܌ठǶ Ǵ٢܊ݹϐ
ȐStaphyl .ȑکεဉఎ ࡋᆶ٢ύᡏ ߚᖏࠠ٢܊
ৣᖏບᘐइ ബ܄ီண
abomasum, 2008ȑǶ
ccus agalac
தـϐ٢ բΓག Կ҂གࢉϐ
ࢉԿঁФ ᔈǴԶઇ ٢܊ϐ่
03ȑǶҗܭ٢ నύϐൂਡ ϐᡏಒझኧ
ϐЬाठੰ
lococcus au
ఎȐEsch ᡏಒझኧࣁ٩
܊ݹȐsubcl इᒵࡰрǴ गݹȐtrau
DAȑ15.9%
ϟಏЬाੰ
ctiaeȑǺ
٢܊ݹठੰ
གࢉ٢ϐ٢ ϐ٢ǶӢԜ ФဂǴՠεӭ ઇᚯౢ٢ಔᙃ ёૈቹៜԛ
٢ဏڀԖؼ ਡౚߡε ኧȐsomatic
༾ғނࣁค ureusȑٿ hericia Coli
٩ᏵǴۘё linical mast
٢܊ݹϐว umatic reticu
%วғ
ੰচӵΠ
চǴЬाӸ ٢ҊԦࢉࡕ Ԝӧཕ٢ፁ ӭឦܭߚᖏ ᙃǵफ़եౢ
ԛݜ٢ය
ؼӳϐՈనٮ ໆΕ٢ဏ cell counts,
ค٢ౚ
ٿᅿॠើМ
iȑځдੰ
ёӆϩ itisȑٿεᜪ วғऊࣁ
ulitis, TRȑ
ٰளଯǴࠅ ΠǺ
Ӹӧςགࢉ
ࡕǴߡԋࣁ ፁғόؼϐ
ᖏ܄ੱރ
ౢ٢ໆ܈ಖԿ ϐ߄ǶȐH
ٮᔈаගٮ ဏύǴᏤठ , SCCȑǴ߯
ȐStretoc
܄Ǵа
ੰচȐߋ ϩԋᖏࠠ
ᜪȐഋǴ199 ࣁ 8.7%Ǵᗨ 21.7%аϷ ࠅࣁ٢Фౢ
ࢉޑ٢Ǵ ࣁኞੰ
ݩΠǴค Ƕಒжᖴ Կค٢Ƕᗨ Hogan and S
ٮӝԋ ठགࢉ
߯Ӣ٢
occus
аϷФ ߋǴ
ࠠ٢܊
98ȑǶ ᗨ҂ၨ
ϷಃѤ
ౢࡕి
ѿ
ޑ൞ ค٢
ᖴቲނ ᗨၨϿ Smith,
ߎဟౚ
! ߎՅ բҗ
ࡕΕ٢ سઇᚯǴ
Զقߎ
फ़ե 15%ϐ
߾ஒԋค
ᡖዀȐMy
! ᡖዀ
ଫǹऩ੬ဂ ςᎁڙགࢉ
ᕍਏ݀ό٫
εǴࡺஒ
εဉఎȐ
! εဉఎ
ǴЬाྍ
ޑѤ७Ǵӧ Їଆϐੱރ ฯ⫒ǵག ԝΫǶӢε ຬၸঁД Smith, 2003
ౚȐStaphy Յဟౚ
җᔒ٢ၸำ ٢ဏಔᙃǴՠ
ԋ
ဟౚၨ
ϐ٢ౢໆǶ คݤൺচϐ
Mycoplasma
ၨॊٿᅿ ဂܭอਔ໔ϣ
ࢉǶڙགࢉϐ
٫Ƕҗܭᡖዀ
ԋ၀٢ϩ
Eschericia
ఎဂࣁॠ
ྍԾނ௨ݧ ӧଳ٢යٿ
ރࣁФଫϱ གǴऩ҂Ϸ εဉఎЇଆ
ДǴோ܌
3ȑǶ
ylococcus a
தᕷ٠ғ ύࢉ๏҂
ՠӧ҆Фي ԋᖋݰ٠ౢ
ၨౚϐ གࢉ߃ය ಔᙃཞ্Ȑ
spp.ȑ
ᅿ༾ғނϿ ϣමᛈวڥ ϐ҆Фౢ٢ ዀ٬ག
܊ϐ҉Φཞ
ColiȑǺ
ើМ܄
ݧނǶ
ٿຼᆶϩ
ࡕ१ኅቲ๊
Ϸਔݯᕍ߾
ଆϐੱރܴ
ԋϐᖏ܄
aureusȑǺ
ғߏܭФଫ
҂ڙགࢉϐ يᡏځдՏ
ౢғ่Ǵջ ϐ্׳ࣁ
ۘёၸݯ ȐHogan and
ϿـǴோځ
ڥ֎س੯ੰ
٢ໆܴᡉΠ གࢉ٢܊ᠼᆢ
ཞѨǶ
ǴӸӧܭ٢
ԶقǴଳ٢
ٿຼϐག
๊ǵวǵ
߾ஒԋՉ
ܴᡉЪёԐය
܄٢܊ݹ՞
ϐ٢ᓐྎ
٢Ǵܰӧ ՏҭёӸࢲ ջߡࢂࡼѺ ᝄख़Ǵࣴز ݯᕍԶࡠൺ d Smith, 20
ࢉ܄ཱུଯ
ੰ܈ᜢဍ Πफ़Ǵགࢉ
ᆢϯǴԋ
٢Фްᕉნ ٢ය໔ޑε གࢉКٯࣣ
Јၢኧቚу Չ֚ᜤǴԿ
යݯᕍǴࡺ
ᕴགࢉੰٯ
Ȑteat canal ӧ٢ᓐҜጥ
ࢲǶགࢉࡕ
Ѻלғનҭᜤ زᡉҢѳ֡
Ǵฅѿᘉ 03, 2003ȑǶ
ଯǴёགࢉҺ ဍεਔǴߡሡ
ࢉ߃යߡЇ ԋ٢܊ᖋဍ܈
ნύϐᕨߡ܈
εဉགࢉК
ࣣܴᡉϲଯ уǵΠฟߡ ԿФଫςคݤ
ࡺӭࣁอයག ٯϐ 30%а
lȑǴၸᐒ
αϷ٢ᓐ
Ꮴठ٢܊
ᜤаਥନǴ
ᓐགࢉФ ᘉණԿঁ
Ƕ
ҺՖԃសቫ ሡѦݙཀ ЇวНဍЪ
܈٢ဏరЃ
܈ӚԄӦ݈
Кٯऊࣁݜ ଯǶεဉఎ ߡ ǵ٢܊ဍ ݤઠҥਔஒ གࢉǴε
аȐHogan ᐒఓ܈
ᓐᆅቚ
܊Ꮴᆅ
ࡺ
Фଫஒ
ঁ٢܊
ቫϐФ ཀࢂց Ъځݯ Ѓ่ဍ
݈პ ݜ٢ය ఎ܌
ဍǵ ஒᏤठ
ϩό n and
Οȑᖏ܄٢
ၸԺᢀ ՅԖܴᡉׯᡂ ༧ǵՈనᏉ Ԝ໘ࢤౢғ
Ѥȑߚᖏ܄
ߚᖏ܄٢ ኧٰղۓǴځ ӧӢηǴ٠
Ъᜤаບᘐ ݹ܌ԋϐ
܌ҢȐRob
٢܊ݹȐcli ᢀჸ܈ᙖҗ ᡂǵဍǴ ᏉڰނԶ
ғੵภϷό
܄٢܊ݹȐ ٢܊ݹᗨค ځགࢉࣁ
ԋ٢ౢໆ ᘐǴӧᔮ ϐཞѨଯၲ
bert et al., 2
inical masti җ౦தϐ٢Ҋ
܈ᄗਔ
ԋФ٢ಔԋ όགǶ
subclinical คݤаԺޔ ࣁᖏ܄٢܊
ໆᆶғ٢ࠔ ᔮࣁ࣬ำ
ၲ 180 ऍϡ 2005ȑǶ
itisȑǺ Ҋཱུܰղۓ ਔёགಔ ԋҽϐׯᡂ
l mastitisȑǺ ޔௗղۓǴ
܊ݹޑ 15
፦ϐΠफ़Ƕ ำࡋϐཞѨ ϡаǴԃ
ۓᖏ܄٢ ಔᙃрฯ ᡂǴࣣࣁᖏ
Ǵՠёа
Կ 40 ७Ǵ Ƕߚᖏ܄
ѨǶਥᏵ
ԃӢ٢܊ݹ
܊ݹϐԖค ϯՏǴа
܄٢܊ݹ
ၸϩᚆੰচ ࣁᙯᡂԋᖏ ٢܊ݹߏය
ीǴऍ୯ѳ ݹЍр߾ຬၸ
คǶऩ٢܊
аϷ٢Ҋύ ݹϐੱރǶ
চ܈ीᆉ ᖏ܄٢܊
යӸӧܭݜ ѳ֡ᓐ٢ ၸٿሹऍϡ
܊Ѧᢀ ύ֖Ԗ Фଫ
ᆉᡏಒ
܊ݹϐ ݜ٢ಔ ٢ФӢ ϡǴӵ
ऍ୯ी
le 1 Evaluat
Ca Yields r
Milk Earlier Fewer de Pharma Veteri Manp
To
ीԃᓐ tions of the
auses reduction
discard renewal epreciation aceuticals
inarians power
otal
Ӣ٢܊ݹϐ every-year
ϐཞѨ loss caused
Loss ($
116.10 24.44 13.60 9.94 9.68 4.84 2.42
181.02
炷modif
d by mastitis
$) 0 4 0
2
fied from Ro
s per cow in
% 64 14 8 5 5 3 1
100
obert et al., n USA
4 4
0
, 2005炸
ϖȑ٢܊ݹϐ Ѧᢀᔠ
! хࡴ٢ Ѧᢀǵჹᆀ
ࢂցၸଯǵ Ԗฯ༧ǵဍ ᚑՅϷ֡፦
Ԑวᆶݯ
CMTᔠᡍݤ
! ԜݤҔ Ԁ٢܊ݹ၂ ᙃಥပϐ
Չಔᙃϐঅ ٢ဏಔᙃύ ٢ύᡏಒझ ٠όڀෳۓ ჴҔϐຑ
ᏤႝࡋȐele
! Ꮴႝࡋ 1cm2ᘐय़ ॶୃৡऊ
ྕࡋୃৡǴ ʚ ਔځᏤ
ϐதҔᔠෳ
٢Фيᡏ଼ந ᆀ܄ᆶελࢂ
Ԗคੵภϸ ဍǵᡂ܈
፦ࡋࢂց҅
ݯᕍǴࡺၨ
ݤȐCaliforn Ҕٰຑ٢ ၂ᡍȐCalifo
ҜಒझϷ অံǴѿ٢ ύǴԋᡏ झኧऩຬၸ
ۓᡏಒझኧ
ࡰǶ
ectrical con ࡋ(Electrical Ǵߏࡋ 1cm ऊ 1.9%ǴӢ ٠а 25ʚ
ႝࡋϟܭ
ෳБݤ
நϐᢀ
ࢂցԖѦ
ϸᔈǴаϷ
܈่ܴ
҅தǶՠऩ
ܰ۹ౣ
nia mastitis ύᡏಒझኧ fornia Masti
ՈనύқՈ ٢ဏಔᙃӢ
ಒझኧ࡚ᗊ
డϲ 50 ϐሺᏔഢ
nductivity, E Conductivi m ϐనࢊਔ ӢԜᏤႝࡋϐ ʚਔϐਠ҅ॶ 4.0~5.5mS/
ᢀჸᆶ٢܊
܈౦தຝ Ϸ٢܊ಔᙃ
ܴᡉੱރ
ऩࣁߚᖏ܄
ౣǶȐಷǴ2
s test, CMT ኧޑӭჲǴࣁ
itis Test, CM ՈౚǴܭ҅த Ӣബ܈ᎁڙ
ᗊቚуȐ߄
ঁǴջё ഢǴࡺᔠᡍ
ECȑϐෳۓ ity, EC)ࣁన ਔႝߔϐॹኧ ϐໆෳ೯த ॶ߄ҢϐȐ /cm ϐ໔Ǵ
ຎບϷບ ຝǴ٠ᔈҔ ǵ٢ဏኲᆶ Ƕཕ٢ϐ
܄٢܊ݹǴ 005ȑ
ȑ
ࣁനத MTȑǶғ٢ தݜ٢ය໔ ڙੰচག
2ȑȐPhilpo ёᇡۓ၀Ф
БԄᙁߡϐ
ۓ
నᡏႝߔॶ ኧǶྕࡋ தܭ 25ʚਔ ȐNielen et a
ǴФ٢ύቹ
ບǶ٢܊ᔠ Ћບག
ᆶ٢ᓐࢂց҅
ϐ٢ᔠҭ
߾ၨόܰ
த٬Ҕϐᔠᡍ ٢ύᡏಒझΏ
Ҝಒझ
གࢉǴՈύқ ot and Nick Фଫςᎁڙག
ϐ CMT ߡԋ
ॶϐॹኧǴۓ ࡋׯᡂឪМ ਔۓྕෳۓ al., 1992ȑǶ
ៜᏤႝࡋॶ
ᔠᔈᕉ៝
٢܊Ҝጥ
҅தǴ٢܊
ҭёаΑှ
ၸѦᢀᔠ
ᡍБݤǴᆀ ΏྍԾܭ٢
Ծฅಥပ қՈౚߡ
kerson, 198 གࢉǹՠӢ ԋࣁᅿ໔
ۓကࣁႝࢬ 1ɗਔǴᏤ Ǵց߾ሡा
Ƕ҅தФ٢ ॶελനЬ
៝٢܊
ጥྕࡋ
܊ࢂց
ှ٢Ҋ ᔠϷ
ᆀࣁу ٢ဏಔ ပϷ
Ε 85ȑǶ Ӣ
໔ௗЪ
ࢬ೯ၸ Ꮴႝࡋ
ाਠ҅
ӧ 25 Ьाࣁ
Na+, K+ک C ᆶᡶᜪӅӕ
ႇᚆη෧Ͽ ٢ဏ๚ύǴ
ᏤठФ٢ et al., 1992
ԶԋФ٢
Ԗ٢܊ݹ
٢ύ༾ғނ
Фଫр
ុΟϺࣁ
߾ёՉ༾
ᕴ٢Ȑbulk
Cl-Οᅿᚆη ӕፓϐǴฅ ϿǴԋ٢ဏ ᔆံᅖᓸ ٢ύ٢ᑗک
ȑǶФଫག ٢ϐᏤႝࡋ ݹǴࡺёᙖ
ނϐᎦ рᖏ܄٢
ࣁ୷ᘵᕍำ
༾ғނᎦ
milkȑύᡏ
ηϐᐚࡋǶ ฅԶ٢܊
ဏҜಒझ ᓸϐόىǴठ
K+ ޑᐚࡋ གࢉ٢܊ݹ
ϲǹځ җෳۓᏤႝ
٢܊ݹਔǴ ำǹऩ٢܊
аղᘐࢂց
ᡏಒझኧᆶ
҅தݜ٢ਔ
܊ݹวғਔ झᅖբҔϗ
ठ٬Ф٢Ꮴ ࡋफ़եǴԶ ݹਔǴ٢ύϐ ځᏤႝࡋຬၸ
ႝॶՉղ
Ӄࡼаቶ ݹੱރ٠҂
ցሡ௦ڗځ
ᆶғኧϐᔠ
ਔǴ٢ဏ๚
Ǵ٢ဏҜ ϗǴՈన Ꮴႝࡋวғᡂ
Na+ ک Cl ϐ໊ᚆηᆶ
ၸߐᘖॶ 5 ۓ٢܊ݹϐ
ቶਏ܄ᛰᏊ
҂ܭԿΒ дଞჹ܄Б
ᔠǶ
๚ϣᅖᓸϐ Ҝಒझڙཞ నύϐ໊ᚆη
ᡂϯǴҭջ٢
- ᚆηޑᐚ
ᆶෛᚆηᐚࡋ 5.6 mS/cmਔ ϐԖคǶ
Ꮚϒаݯᕍ ΒঁᕍำࡕԖ
БԄуаݯ
ϐѳᑽӄᒘ Ǵ٬٢ᑗϩ ηᆶෛᚆη ٢܊ݹวғ ᐚࡋቚуȐN ࡋஒϲ ਔǴջёղ
ǴаԐఁӚ Ԗܴᡉϐׯ
ᕍǶ
ᒘ٢ᑗ ϩݜϷ ηᅖΕ ғਔǴ Nielen ϲǴӢ ղۓځ
Ӛԛ ׯ๓Ǵ
Ф٢ᡏಒ le 2 Relation
Somatic cell
ಒझኧ֖ໆᆶ nships betw
l countsȐ×
14 ~ 19.5 22.5 ~ 38 42 ~ 120 128 ~ 228
ᆶ٢ౢໆཞ ween somati
104 /mLȑ
炷
ཞѨԭϩК c cell count
炷modified f ᜢ߯
ts and loss i
Milk yi
from Philpo
in milk yield
elds lossȐ%
5 8 9 ~ 18 19 ~ 25
ot and Nicke ds
%ȑ
erson, 1985炸
Ϥȑ٢܊ݹϐ ٢܊ݹவག ԜǴऩ׆ఈૈ
ϐᆅ૽ግǵ ёԖਏफ़եੰ
ཕ٢ᕉნϐ
! ନཕ٢ ࢱҭ࣬ख़ ᗉխੰচ
ҭ࣬ख़ा
ᏹբΓϐ
! ନᔈௗ
ғ૽ግ࣬
ϐФଫ٢܊
٢܊ϐమዅ
! ཕ٢
੮ܭഢύ ӆаરτᔔ ࡕϐ႖ᚆ܄
ଳ٢යϐೱ ϐᕍำࣁ٫
ၲډԖਏϐ
ϐݯᕍᆶ
གࢉ҆Фஒ
ૈԖਏڋ ǵଳృϐ٢܊
ੰচ༾ғނ
ϐమዅᆶഢ ٢ਔϐᐒఓᓸ ख़ाǶФဂύ
ၸᐒᏔ
ाǶ
ϐ૽ግ
ௗڙֹ๓ϐཕ
࣬ᜢᖱಞǴִ
܊ബǴۘё
ዅᆶᅄ
ሡᄇۭమዅ ύǹ٢ᓐܭႣ ᔔଳ٢ᓐࡕ
܄ᛰᅄё ೱុ܄٢ᓐ႖
٫ǶฅӢӚቷ ϐᛰਏ݀
ڋ
ஒੰচኞ ڋࢉ܄٢܊
܊Ϸ٢ᓐ٠ ނޑණኞǶ
ഢᆢៈ
ᓸΚሡᛙۓ ύऩςԖᖏ
ࢉԿ଼ந
ཕ٢௲ػ૽
ִ๓ঁཕ ёٛЗठੰ
ዅФଫϐ٢ Ⴃࢱᔒక٢
٢݆Ǵ ёԖਏफ़ե ႖ᚆᅄё ቷจϐᛰ
ȐHogan an
ኞԿ҂གࢉ
܊ݹǴཕ٢ ٠ܭཕ٢ࡕ
ۓаᗉխԋ
܄٢܊ݹ நϐФଫǶԜ
૽ግǴჹഢ ཕ٢ᕉǴନ
ੰচၸᏹբ
٢܊ᆶ٢ᓐ
٢όҔǴ٠ ё෧Ͽ੮ӧ եӢᕉნύε ёԖਏڋᕉ
٬Ҕࣣόᅰ nd Smith, 20
ࢉФଫǴځ
٢ᕉნϐమ
ՉᅄǴ
ԋނ܄ബ ݹϐФଫǴᔈ ԜѦǴߥ
ഢᏹբԖ
ନёᗉխ
բΓܭФ
ՏǴᗉ ٠ᛰ٢ᓐ٬
ӧ٢ᓐ߄Ҝ εဉ܌
ᕉნЇଆϐ ᅰ࣬ӕǴᒥ 003ȑǶ
ࢉ൞ϟЬा
ዅᆶഢϐ аϷႩᔼ
ബϐѦǴཕ ᔈஒځཕ٢ԛ
Фଳᔿమ
ۓዕࡋѦ
ԋӢᐒఓᏹ ФဂύҬΰག
ᗉխӢᕨߡ
٬ځᆶࢥ Ҝޑ༾ғނ
ԋϐ٢܊
ϐ٢܊ݹགࢉ
ᒥൻ٬Ҕᇥܴ
ाࣁཕ٢
ϐᆢៈǵᏹ ᔼᎦᆶࣝभ
ཕ٢ࡕ҅
ԛׇፓԿന మዅ٠ۓය
ѦǴۘሡௗ
ᏹբόؼ܌
གࢉϐёૈ
ᕉნύᠢ ࢥНௗ 30
ǶԜѦǴܭ ݹགࢉཥੰ
ࢉǴа 2 Կ
ܴ҅ዴᏹբ
ഢǶ ᏹբΓ भǴ
҅ዴమ നࡕǴ යࢥ
ௗڙፁ
܌Їଆ
ૈǶ
ᠢԦᅉ
ࣾǴ ܭཕ٢
ੰٯǶ Կ 5 В բۈૈ
ࣝभᆶႩ
! ߈ԃٰ
෧Ͽ٢ᡏಒ ᡉҢǴݜ٢ ఎϐགࢉ
ܭଳ٢ය໔
གࢉ܌Ї ғન A ǵᆢ
ԋᕉნ܄
Չి؎ȐHo
ᔼᎦϩ
ٰ࣬ଯКٯ ಒझኧ٠ගଯ ٢Фံкೈữ
ࢉำࡋǴЪё ໔ࡼѺ̆-5 ε ଆϐᖏ܄
ᆢғન Eǵ
܄٢܊ݹϐว ogan and Sm
ٯϐчऍ٢ ଯౢ٢ໆǶ ữለɡᎋё ё٬҆Фܭ εဉఎࣝ
܄٢܊ݹੰ
ǵ-चᡀጱ วғቚу
mith, 2003ȑ
٢ФࣣᗯႩೈ
ճҔεဉఎ ёׯ๓٢ᆅف
ܭװࢥ၂ᡍ
ࣝभϐ҆ФႣ
ੰٯϐኧໆᆶ નǴаϷ༾
уǶऩФଫр ȑǶ
ೈữለɡᎋ ఎװࢥᆶ ف፦નϐ
ύԖၨ٫ϐ ႣٛݙǴ ᆶำࡋǹ࣬
༾ໆϡન⃯
рុϐ
ᎋǴёफ़ե٢ ᆶज़ڋᎋឪڗ
ԋǴ٠ё௨ ϐࡠൺำࡋ
ёԖਏफ़ե
࣬ჹԶقǴႩ ǵልکᎋ
ϐᄌ܄٢܊ݹ
٢ϣགࢉว ڗໆϐ၂ᡍ ௨ନ٢ᆅϣ ࡋȐಷǴ201 եݜ٢Ԑය Ⴉᙂύऩલ
ނ፦Ǵ ݹǴ߾၀Ե
วғǵ ᡍ่݀
ϣεဉ 11ȑǶ යεဉ લЮᆢ
ࣣ
Եቾ
ғ٢ኬࠔճ
ȑғ٢٢ໆ ٢ໆ
٢ໆࣁ ϐݜ٢Ԕጕ ݜ٢ФՉ
٢ᑗ
! ٢ᑗऊ ϐᇙԋΏ
Ȑlactose in
٢િ
! Ѡ
ࣁಉᠼᆢ௦
િǴ⟻ᜪ
િΠफ़
٢ೈқᆶ
! ! ٢ೈқ ճҔႩᙂύ
҅࣬ᜢϐ ٢፦ᔠᡍф 11 mg/dlȑ
፦ࣁ 3.2
ճҔϐϟಏ ໆᆶಔԋϩ
ࣁၭύВ ጕǶၸ٢ Չౢໆଯե
ऊ՞٢ಔԋϩ
ၸ٢ለ
ntoleranceȑ
ើФϐ٢િ
௦१ໆϐᅱ ᜪᆒ߾Ӣ Ƕ
ᆶֿનේ
қᆶේϐϩ ύϐಉೈқ ϐ࣬ϕѳᑽ фૈǴԾ 20 ǵ҅தȐ11 2ʘѰѓǴֿ
ಏ
ϩϐϟಏ
Вനࣁ၁ၩ
٢ໆϐ၁ჴ
եϩဂаό
ϩϐ 4.67%
ஒ٢ύϐ٢ ȑதـܭ
િѳ֡ऊ ᅱǶቚуң
ၨܰౢғЧ
ϩЬाᆶݜ
ϩှ
ᑽރᄊǹՉࡹ
002ԃ 12 Д ɴ17 mg/dl
ֿનේ֖ໆ
ၩϐኧᏵǴ
ᒵёԖਏ όӕሡϐ
%Ǵځख़ा܄
٢ᑗᙯඤԋ
ᅿΓᆶߚဴ
ऊࣁ 3.7ʘǴ ңҁࣽᆶ
Чለિެ
ݜ٢Фૈໆ
ှӝԋ༾ғ
ࡹଣၭہ
Д໒ۈගٮ lȑϷၸଯ ໆ߾ऊࣁ 15
ၸჹ٢ໆ ਏి؎ౢໆե ϐВᙂǴаၲ
܄ᗨόϷ٢ ԋ٢ለаफ़ե
ဴΓǴࡺ
ႀၭЊё٩
ёගଯච
ެለࡺܰᏤठ
ໆϷೈқ፦௦ ғނϐೈқ፦
੬ౢ၂ Ф٢ֿનේ
Ȑɧ17 mg/
mg/dLȐ
ໆϐϩёᛤ եΠϐ٢Ф
ၲ׳Ԗਏ
ೈқᆶ٢િ
ե pH ॶǴЪ
ࡺϝࣁෳۓϐ
٩Ф٢ύϐ චว܄Όለ ठዦगวሇ
௦१ໆ࣬ᜢ
፦ਔǴޑ ၂ᡍ܌ཥԮϩ
ේϐኧᏵǴё dlȑǶԶ
Ǵ2003ȑǶ
ᛤᇙൂݜ ФǴ٠ёჹ
ϐᆅǶ
િǴᓬ Ъ٢ᑗόऐ ϐࡰϐ
ϐ٢િϩ
ለԶճܭගଯ ሇ౦தǴԋ
ᜢǶၸ༾ғ ޑճҔᆶૈ
ϩ܌ࣁуம ёϩԋၸե ԶقǴФ٢ Ƕ
ݜ٢Ф
Ԗ
ᓬႀ٢ ऐڙੱ
Ƕ
բ ଯ٢ ԋ٢
ғނ!
ૈև ம DHI
եȐɦ ٢ೈқ
٢ᕴڰނ
! ! ѱय़
፦аϷᆢғ ύᕴڰނ ჹගଯᕴڰ ၸեਔǴё ݹ੯ੰа
ғ٢ᡏಒझ
! ! DHI ᔠ
֖ໆҭࣁၭ
ޕൂݜ٢ ݜ٢Фϐᡏ
ΒȑߚߟΕԄ ӧဂᒪ ҭ܈ނ
ғ ނ ϐ ࣴ oninvasive s
߈ԃٰǴ ӵᕨߡǵԳ DNA ٰྍǴ Ոన܈ಔᙃ
AȐmitocho ϐࣴزǴӧ
ނ
ϐ٢ቷӭ ғનԌନ ނǶᕴ٢ڰ ڰނǴ
ёቚуңҁ аٛݜ٢ໆ
झኧ
ᔠᡍൔύ
ၭ໔ϩભϐ ٢Фϐᡏಒझ ᡏಒझኧܭ
ԄڗኬБݤ
Ꮲϐࣴز ނǴԜᜪೀ
ࣴ ز ߡ ໒ sampling me
ςวр࣬
ԳЛǵᆒన ǴځҞޑࣣ
ߟΕԄڗ ondrial DNA ӧಒझਡϣ
Ԗϩคિ
ନНϩᆶ٢િ
ނࣁଳނ
Զق٢ύ ҁࣽаׯ ໆΠफ़Ƕ
ύϐᕴᡏಒझ ϐख़ा٩Ᏽ झኧǴԜ
డϲғ٢
ݤϐϟಏ زǴނ
ೀၸำ֡
໒ ۈ Ⴔ ଆ ethodsȑ(W
࣬ӭϡϯ నǵЛᎳǴа
ࣁ׆ఈၸ ڗኬϐ DNA A, mtDNAȑ
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િڰނϐ
િϐଳނǶ ނ፦௦१ໆ ύϐᕴڰ
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झኧࣁႀၭ
ᏵϐǶҗܭ
ϩኧᏵߡ ٢ύ֖ໆεܭ
ނ۳۳ሡा
ჹނᡏ ଆ ඹ ж ϐ ڗ Woodruff, 19 ϯϐߚߟΕԄ
аϷғ٢
ၸԜᜪኬҁ A ٰྍǶߚ ȑϐ PCR ቚ NAϐϩ
ϐၗǴх֖
ค٢ڰނ ໆϐࡰǴග
ނऊࣁ 12 ଳނ፦௦१ໆ
ၭന٫ᑔᒧϐ ܭᖏ٬Ҕ ߡᡉளѦ ܭ 50 ঁ
ਂਆ܈ᅉ ᡏҁيԋ ڗ ኬ Б ݤ 993) Ƕ ԄڗኬБݤ
Ƕคፕࢂ
ҁаڗளؼӳ ߚߟΕԄኬ
ቚ൯аҔܭ
ϝԖۓ
֖٢ೈқ፦
ނᆶ٢િ
ගଯଳނ፦
ʘǶऩᕴڰ ໆόىਔǴ
ϐࡰǴԶ ϐ CMT ᔠ Ѧख़ाǶ
Ǵջӈࣁ
ᅉ੮ǵ
࣬εϐᆙ Ǵ ҭ ջ ߚ ߟ
ݤаࣁނ
٬ҔՖᅿߚ ӳࠔ፦ϐ D ኬҁςԋфӦ ܭᅿձ᠘ᆶ
ۓޑ֚ᜤࡋ
፦ǵ٢ᑗǵ
ӝٳջࣁФ
፦௦१ໆջё ڰނᆶ٢
ҭሡݙཀᒙ
Զᕴ٢ᡏಒझ ᔠෳݤ٠ค
ԶقǴऩ ࣁ٢܊ݹФଫ
ǹԶคፕ ᆙॐǶӢԜ ߟ Ε Ԅ ڗ
ނᡏ DNA ߚߟΕԄኬ DNAǴԶ ӦճҔܭಈ ᆶᅿ໔ᒃጔ Ǵӵᒃη᠘
ނ Ф٢ ё࣬
િ
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܈פ൨ёૈϐ ࣁ२ाፐᚒ ᙃࣁҞനத
ԋۓ ҭࣣԖ॥ᓀǶ
ख़ाϐፐ ໆϐᡏಒझኧ
ٰྍڀԖჴҔ ܭғ٢ኬҁЬ ჹၨࣁֹԶ
ΟȑԾғ٢ኬ аݤܜ
Ԗ᠙ܭ ރຑǴL ύܜڗ DN ᙦǴՠ
ۑǴ܈ݜ
૽ግ܈ڐշ झ܈ёԋࣁ
Ϸঁᡏ᠘ۓ
ࣁߚߟΕ
ӭϩηᒪ ϐᒪ܄੯ Ƕᒿಔᙃ த٬Ҕϐ୷ ۓำࡋϐᆙ ǶӢԜǴӵՖ
ፐᚒǶ٢Ҕ ኧǴ܈ё
Ҕ܄ଯǵჹ Ьाڗளٰ
Զճܭεೕ
ኬҁܜڗ D ܜڗғ٢ኬ ܭаՈనύқ
LipkinΓ NA ϐБݤ
డϲѳ֖֡
ݜ٢໘ࢤԶۓ շջёՉ ࣁ DNA ϐќ
ۓȐChu e
ΕԄڗኬϐٰ
ᒪࣴزמ ੯ੰǹԶӧፏ
ᙃٰྍόӕ
୷Ӣಔ DNA ᆙॐǴᏹբΓ
ՖၸՈన Ҕᔮނ
ࣁќ୷Ӣ
ނᡏᆙॐ
ٰྍࣁФଫ܈
ೕኳϐຑК
DNAϐϟಏ ҁ DNA қՈౚࣁ
Ȑ1993ȑׯ ǴځࢬำҢ
֖ໆҭऊౣ
ۓǶФ٢ϐ Ǵ٬ځܭኬ ќߡճϐ
et al., 2006ȑ
ٰྍ
מೌᆶБݤճ ፏӭϩמ
Ǵҭςว
AٰྍǹฅԶ Γҭሡ
నᆶԼԺಔ
ВԐఁࣣ
Ӣಔ DNA ϐ ॐၨեǴаϷ
܈Բଫ٢ КၨȐPsifi
ಏ
ࣁ DNA ٰྍ
ׯؼΑவᆒ Ңཀӵკ 2
ౣࣁ 104Կ 1 ϐ௦ࣁ
ኬҁڗள
ϐඹжٰྍ
ȑǶ
ճҔܭނ מೌǴ׳ଯ
рόӕϐ ԶǴՈనϷ
ၸ࣬ޑ ᙃаѦޑ
ࣣሡჴࡼཕ ϐඹжٰྍ
Ϸԋҁ࣬ჹ ٢Ҕᔮ
idi et al., 20
ྍၨόҔ నܜڗ DN ǶФ٢ύ܌
107ঁǴຎځ ВٯՉπբ
׳ࣁቸ܄Ƕ Ƕ
ނᡏғౢ܄ૈ
ଯࠔ፦ᆶى ڗኬБԄ ϷԼԺಔᙃϐ
૽ግǴჹ
Տڗள୷ ٢բǴЪ
ྍǶағ٢
ჹၨࣁեሽ
ނǴࡺځғ 010ȑǶ
ܭՉεೕ
NAϐБԄࡌ
܌֖ϐᡏಒझ ځ଼நރݩ բǴЪЙሡၸ
ӢԜǴФ٢
ૈϐ܄ރϩ ىໆϐ DNA
ǴԶՈనϷ ϐڗኬჹ
ނᡏ܈ᏹ
୷Ӣಔ DNA Ъғ٢ύ֖
ࣁߚߟΕ
ᓬᗺǶԜ ғౢ܄ރϐ
ೕኳᔮ
ࡌҥவ झኧᗨόϷ ǵ߃ౢ܈
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ϩǴ
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ނᡏ ᏹբΓ ߡࣁ
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ΕԄኬ ԜѦǴ ϐᒵ
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ϐǶPsifi ځύх֖ٿ
ϐٿᅿܜ ՉຑǴځ КǴReal-tim ᡉҢׯؼࡕ ࣁ٫Ƕҗܭ ኬϐؼӳٰ
ಔܜڗғ٢ኬ
Ҕಔܭ ԄҔಔ
ߟΕԄኬ diΓȐ20 ٿಔҔ
ܜڗݤՉ ځ่݀ӵ߄
me PCRϩኧ ࡕϐҔ
ܭԲ٢ύ܌֖
ٰྍǶ
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ࣁ DNA ҁ܌ीǴ 010ȑట၂
ಔǴаϷଞ КჹǴ٬Ҕ
3܌ҢǶ߄ ኧϐ Ct ॶ ಔᏹբำׇ
֖ϐ҅தᡏ
ϐёՉ܄
ڗਔගٮ࣬
ܜڗπڀ ӢԜӵՖᔈ ၂КၨϤᅿ ଞჹٿಔҔ
ҔٰԾ 5 ঁ ߄ύКၨΑٿ
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ׇԖၨ
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ϐߡճ܄
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ᔈҔܭߚߟ όӕБԄܜ Ҕಔϐᏹ
όӕ੬ဂϐ ٿঁόӕޑ
БԄ܌ሡϐ ݤँрϐ 106ঁа
܄ᆶז܄
Ҕಔ ߟΕԄኬҁߡ
ܜڗғ٢ύ ᏹբᡯՉ
ϐ 15 ଫᆟԲ ޑ֎ӀॶКॶ ϐ DNA ܜڗ ϐ߄ǴЀа
Ǵςى
Ǵӭ၂ᡍ ಔӭࣁՈ ߡࣁځЬा
DNAϐᓬ ՉׯؼǴӆ Բࣁෳ၂ ॶǴႝݚϩ ڗਔ໔Ϸԋ аՈనҔϐ
ࣁߚߟΕ ᡍࣣ໒ Ոన܈
ाፐᚒ ᓬӍǴ ӆᆶ
၂а
ϩኧຑ ԋҁǴ ϐಔ ΕԄڗ
კ F m
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Figure 2 Flo methodsȐL
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4
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уΕ 10
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ដ 1000 ×gǴ4ʚ ᆶғ٢ໆϐ 1/
ដ 1000 ×gǴ4ʚ
మనࡕуΕ 10
lysis bufferܭ
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., 1993ȑ/
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0 nMϷ 0.2 mg
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oodȑǴ
ྋ!
Lipkin et al.
m milk by tra
g/mL!
., 1993ȑǶ!
aditional
Cost per sample (€)
Process duration Ct valuecElectrophoresis score 260/230 nmb 260/280 nma Extraction method 2.7 1.15 hours 1.15
24.3 (0.40) 23.7
45.0 (10.1) 1.67 (0.14) 1.73 (0.03)d Nucleospin¯ Blood Kit ¯ 2.9 2.9
1.15 hours 2.15 h
23.7 (0.41) 22.8 (046)
48.0 (9.6) 89.1 (10.0)
1.67 (0.14) 2.10 (0.14)
1.71 (0.03) 1.80 (0.03)
Nucleospin¯ Tissue Kit Modified Blood Kit 3.1
hours 2.15 hours
(0.46) 23.4 (0.41)
() 54.5 (10.1)
() 1.85 (0.14)
() 1.74 (0.04) Modified Tissue Kit 2.0 1.5 days
25.0 (0.39) 28.5 (10.52) 2.71 (0.14) 1.55 (0.03) Phenol-Chloroform 1.5 2.15 hours
24.1 (0.49) 11.5 (12.5) 1.43 (0.18) 1.43 (0.04) In-house protocol Rtifbbt260d280tlttitt (Modified from Psifidi et al., 2010) a: Ratio from absorbance at 260 and 280 nm to evaluate proteins contents. b: Ratio from absorbance at 260 and 230 nm to evaluate salts contents. c: From real-time PCR. dStddd: Standard error.
ФଫЬाಔ
ȑЬाಔᙃ Ьाಔᙃ࣬
Ьाಔ genesȑࣁ
ύǴځфૈ
ȐMHC cla
ࣴزύගр complex 2 ځύΓᜪ leukocyte a
୷ӢȐswin BoLA୷Ӣ
MHCܭ ԜҺٿঁᡏ ѾϐຝǶ ϩηǴϿኧ Ϸᐋރँಒ ηǶόӕܭ ѾȐallelic
ঁᡏࣣё MHC୷Ӣ
ಔᙃ࣬ፄ
ᙃ࣬ፄӝ
࣬ፄӝᡏ୷ ಔᙃ࣬ፄӝ
ᅿৎ܄
ૈᆶխࣝس ass Iȑᆶಃ
рȐBenjam ȑȐკ 3Aȑ
MHC ୷Ӣ antigen, HL ne leukocyt Ȑbovine le ܭঁᡏϐ໔ ᡏ໔ϐ MHC ǶԜѦǴӧલ ኧӵ B రЃ ಒझȐdendr ܭځдխࣝౚ
exclusionȑ ёӕਔ߄
ȐBenjamin
ፄӝᡏ୷Ӣϐ
ӝᡏϐϟಏ
୷Ӣ
ӝᡏ୷ӢȐ
܄୷ӢȐMH
ϐלচև Βᜪ MHC mini et al.,
ǴϐࡕኧΜ ӢՏܭಃϤ LAȑȐკ 3B te antigen, eukocyte an ໔ڀԖ࣬ଯ
C߄ࣣό લЮᇨᏤӢ
ౚȐB cells ritic cellsȑ ౚೈқϷ T ȑϐຝǴ
ٰԾРБᆶ ni et al., 200
ϐࣴز
Ȑmajor hist HC genes fa և࣬ᜢǶ CȐMHC cla
2003ȑǴ
Μԃ໔ځӕ ϤဦࢉՅᡏ
Bȑǹӧଫ SLAȑǹӧ ntigen, BoL ଯࡋϐ୷Ӣ όᅰ࣬ӕǴࢂ
Ӣηϐݩ sȑǵૅဏ
߾ӕਔ٠ TరЃౚڙ
ԶࢂаӅᡉ ᆶ҆Бϐ M
03ȑǶ
ocompatibi amilyȑǴቶ
MHC Ьा
ass IIȑǶM
ᆀϐࣁ H-
ྍ୷Ӣҭഌ ᡏǴᆀࣁ
ଫ߾Տܭಃ
ӧФଫύՏܭ LAȑǶ Ӣӭኬ܄Ȑg
ࢂࡺཱུܰ
ΠǴεϩ
ҜಒझȐt ٠ុ߄
ڙᡏǴMHC ᡉ܄Ȑcodo MHC ୷Ӣ
lity comple ݱӸӧܭૉ
ाϩԋٿᜪ HCനԐܭ 2୷ӢȐhi ഌុӧځд
ΓᜪқՈౚ ΎဦࢉՅᡏ ܭಃ 23 ဦࢉ
genetic poly
ԋಔᙃ౽
ϩಒझ߄ hymus epith
ಃᜪᆶ
୷Ӣ߄٠ ominantȑϐ Ǵคፕࢂಃ
ex genesǴM
ૉނ୷ Ǻಃᜪ M ܭ 1937 ԃӧ
istocompati
ނύว ౚלচȐh ᡏǴᆀࣁ
ࢉՅᡏǴ
ymorphismȑ
ਔౢғז
ಃᜪ M helial cellsȑ
ಃΒᜪ MH ٠คҬඹ୷ ϐБԄ߄
ಃᜪ܈ಃ
MHC
୷Ӣಔ MHC ӧλႵ ibility วǴ uman SLA ᆀࣁ
ȑǴӢ זೲ௨ MHC ȑǵа HCϩ
୷Ӣ௨
ǴҺ
ಃΒᜪ
AA.
B.
კ 3! λ ȐBenjam Figure 3 geneȐB
λႵᆶΓᜪ M mini et al., 2
MHC gene enjamini et
MHC୷Ӣ 2003ȑǶ es of mice a
al., 2003ȑ
ǶA. λႵ
and human.
.
H-2୷Ӣ
A. mice H-
ǹB. Γᜪ H
2 gene; hum
HLA୷Ӣ
man HLA
ಃᜪ MH
ಃᜪ Ȑtransmem ձࣁ AǵB λౚೈқȐ ڀԖѤঁझ
! !
ಃΒᜪ MH
ಃΒᜪ Ϸ DRǹԜ ᆶ 39!lEb ϐ superfamily Ȑinvariant όᡂӧܭ চϩη่ӝ ȐTakeshim
MHCϐфૈ
ѿᎁ ࣁૈϩಒ
фૈаֹ
presentation ٮᒣϐ
לচ 9ঁữ୷ለ
HCϐϩη่
ᜪ MHC ୷Ӣ mbrane glyc
аϷ C Ȑ2 microgl
झѦୱϐ
HCϐϩη่
ᜪ MHC ୷Ӣ ԜΟঁझѦ
ϐऀጢᗐೈ
yȑԋϐ
regionȑᆶ ܭ่ӝ T ಒ ӝਔૈԖၨ
ma et al., 20
ૈ
ᎁڙੰচ
ಒझࢂցς
ֹԋǶԜၸำ nȑǴࣁ MH
ೱՍϸᔈ চࣁϣғ܄
ለߏࡋϐอᴏ
่ᄬϟಏ ӢȐMHC c
coproteinȑǴ Οঁୱǹ lobulinȑа ϩηȐTake
่ᄬϟಏ ӢȐMHC cl
ୱࣣԖ
ೈқǶಃΒᜪ
Ǵᆶಃ
ᆶᡂ౦ၨεϐ झϐۓ C εϐቸ܄Ǵ 08ȑǶ
לচགࢉ
ᎁڙགࢉǴ ำੋלচ HC ϩηܭ
Ƕ
Ǵջӧಒझ ᴏ两Ǵ٠ၸ
class Iȑӝ ǴڀԖΟঁझ ǹԜᜪೈқ аߚӅሽ่ӝ
eshima et a
lass IIȑԖΟ Ϸ ٿᅿ୷ ᜪ MHC ࣁխ
ᜪ MHC ϐ ϐᡂ౦Ȑ CDϩηǴӵ
ࡺ T ಒझё
ࢉਔǴխࣝس аճ T ಒ চঅႬȐanti ܭಒझϣᆶל
झϣӝԋೈқ ၸ TAP-1 Ϸ
ԋϩηໆ झѦୱȐ
፦Ξᆶќ ӝБԄӸӧ
l., 2008ȑǶ
ΟᅿझѦ
୷ӢǴϩձ խࣝౚೈқ
ϐữ୷ለ่
Ȑvariable re ӵ CD8+ᆶ ёᆶ൳Я܌
سջ໒ झՉբҔ igen process לচϐᴏ两
қ䁙ፄӝᡏ Ϸ TAP-2 ၮ
ໆऊ 43 kDa Ȑextracellul ќϩηໆ ӧܭಒझ߄य़
ୱǴϩձࣁ ёӝԋϩη ຬભৎȐ
่ᄬࣣԖଯࡋ gionȑǶಃ
CD4+ǹᡂ౦
܌Ԗᜪࠠϐೈ
ۈ௴ٛፁ ҔǴሡᙖҗ
singȑᆶלচ 两่ӝ٠ၮଌ
ᡏǴ
ၮᒡೈқஒϐ
aϐऀጢᗐ lar domainȑ
ࣁ 12 kDa य़Ǵٿޣፄ
ࣁ DPǵDQ ηໆऊࣁ 4
immunoglo ࡋߥ੮ϐό
ᜪᆶಃΒ ౦߾٬ځ
ೈқ፦לচ
ፁᐒڋஒϐ MHCϐל চևȐan ଌԿಒझጢ
फ़ှࣁऊ ϐଌԿϣ፦
ᗐೈқ ȑǴϩ ϐ 2 ፄӝԋ
Qǵа 6!lEb obulin όᡂ
Βᜪϐ ځᆶל চ่ӝ
ϐྐǶ לচև
ntigen ጢа
ऊ 8 Կ
፦ᆛǶ
ӧϣ፦ᆛύ Կಒझ߄य़ ՈౚѦٰ
ឪΕѮᏘಒ
ှࣁၨอϐ ԋፄӝᡏǴ al.Ǵ2003ȑ
MHCϩηӭ
ಃᜪ
፦ϩηҭ߄ ჹלόӕࠠ
ᜪϐ B ࠠط
ૉݹȐan ҥլМੰȐ ϐੰำว
ύǴᴏ两לচ य़ᆶ CD8+T
ٰלচਔǴ ಒझǴ٠ᆶ ϐᴏ两Ƕಃ
ӆஒ Ƕ
ӭኬ܄
ᜪᆶಃΒᜪ ߄рᜪ՟
ࠠᄊϐלচ طݹȐhepat
nkylosing sp ȐMarek’s di
ࣣᆶ MHC
চஒᒧ܄
T ಒझڙᡏ ջझᏘ ᆶಒझϣϐ
ಃΒᜪ MH ஒϐևԿ
MHC ୷Ӣ ϐଯࡋӭᄊ ǶԜѦǴM titis Bȑǵ่
pondylitisȑ iseaseȑǴک C୷ӢԖᜢ
܄ᆶಃᜪ ᡏՉϸᔈ
ᏘȐphogocy ϐ៶ݰᑼӝԶ
HCӧᚆ໒ϣ ಒझ߄य़ᆶ
ϐᡂ౦ڀ ᄊ܄ǴԶԜᜪ MHC ୷Ӣ
่ਡੰȐtub ǵᆶངੰ
ФଫқՈੰ
ᜢǶȐBenjam
MHC่ӝ ǶԶלচऩ ytosisȑ܈ϣ Զܭ ϣ፦ᆛࡕߡ ᆶ CD4+Tಒ
ڀ࣬ଯϐ ᜪᒪᡂ౦
ຎࣁᆶ
beroulosisȑ
ੰȐAIDSȑ
ੰੰࢥȐbov mini et al.Ǵ
ӝǴӆ೯ၸଯ ऩࣁ༾ғނ
ϣտբҔȐ ܭԜለ܄ᕉნ ߡᆶԜᜪอ
ಒझڙᡏբ
ϐӭᄊ܄Ǵ٬
౦ຎࣁ٬ঁ
ۓགࢉ܄੯ ǵᅫ੯Ȑma
ǹځдނ vine leukem
2003ȑ
ଯ୷Мᡏࡕ ǵೈқ፦ǵ endocytosis ნΠೈқ อᴏ两่ӝ ҔȐBenjam
٬ځ߄ϐ
ঁᡏૈ׳Ԗ ੯ੰ࣬ᜢǴ malariaȑǵሼ ނᅿӵᚊଫ mia virus, B
ࡕଌ
܈आ sȑ
қ䁙फ़ ӝ٠
mini et
ϐೈқ Ԗճܭ ӵΓ ሼޔ܄
ଫϐଭ LVȑ
ΒȑФଫЬा
ФଫరЃౚ ӧФଫ BoLAȑ୷
ಃ 23 ჹࢉ
ӭ࣬౦ϐ Ԝࢤׇӈܭ Ԝࢤख़௨ׇ
al., 2008ȑǶ ቹៜ CD4+ DOAǵDO
ԜѦǴΓ DQౢނǴ
ಃΒᜪ MH ӢТࢤࣣё
ঁ DRA
୷Ӣϩձ
ଯԶຎࣁ ԿϞςԖ
ߏࡋӭᄊ܄
қ፦ႝᗺ polymorphi oligonucleo electrophor ϩ ձ ࣁ ่ ӝ
ा࣬ፄӝ ౚלচ୷Ӣϐ ଫύǴMHC
ӢǴςຎ ՅᡏǶᗨ ϐೀǶ२Ӄ ܭࢉՅᡏ
ׇӈܭФࣽ
ӧ IIa ୱ ᇶշ T ಒझ BǵTAP1ǵ Γᜪϐ HLA
Զ BoLA ୷ HC୷Ӣύ ё߄ȐSch
୷ӢǴΟঁ
ᆀࣁ DRB ࣁၨख़ाϐ
ӭࣴزճ
܄Ȑrestrictio ᗺǵՈమᏢ
ism analysis otide analys esis, DGGE ӝ RFLP
ӝᡏ୷Ӣ ϐᙁϟ
୷Ӣᆀϐ ຎࣁФଫխ ᗨฅ BoLA ୷
ǴӧಃΒᜪ
ϩԋٿঁ
ނӵᆟԲ ୱύǴх֖ڀ
झϐज़ڋ
ǵTAP2ǵLA A୷Ӣܭಃ
୷Ӣ߾߄ Ǵຬၸъኧ hwab et al.
ঁ DRB ୷Ӣ B1ǵDRB2
୷ӢǴЀځ ҔόӕБԄ on fragment Ꮲࣴزǵൂި
s, SSCPȑǵ sis, SSOȑ EȑǴаϷᆫӝ
ϐ PCR-RF
ϐࣁФଫర
ࣝ܄ރᆶ੯
୷Ӣܭ่ᄬ ᜪ MHC ୷Ӣ
ঁၨλϐ
ԲᆶξԲϐ ڀԖЬाфૈ
ᡯǹIIb ୱ AP2аϷ L
ಃΒᜪ MHC ߄ᅿ DR ኧϐ୷Ӣࠠڀ
, 2009ȑǶ
ӢǴঁ܈
Ǵᆶ DRB3 ځࢂ exon 2Ȑ
Ԅଞჹ BoL t length pol
ިᄬӭᄊ
Ԗׇӈჲ ǵᡂ܄ఊࡋ ӝ䁙ೱᙹϸ RFLP ݤ ᆶ
రЃౚלচ ੯ੰϐख़ा
ᄬᜪ՟ܭΓ ӢԖܴ
ୱǴϩձᆀ MHC୷Ӣ
ૈ߄ϐ୷ ୱ߾х֖ D LMP7୷ Cୱӕ R୷Ӣౢނ ڀԖ DQ ϐ
ԶقǴ
܈ٿঁ DQA 3୷ӢǴځ ȐDRB3.2 ୷
A-DRB3୷ lymorphism ᄊ܄ϩȐ ჲਡ㧿ለࠠ
ࡋጤᡏႝݚ ϸᔈȐpolyme
่ ӝ ᡵ ୷
Ȑbovine le
ाᒪংᒧ
Γᜪϐ HLA
ܴᡉϐख़ཥ௨ ᆀࣁ IIa ୱ Ӣҭςว
୷ӢТࢤ DR DYAǵDYBǵ
୷ӢǶ
ӕਔ߄ٿᅿ ނᆶٿᅿа
ϐख़ᙟТࢤ Фଫ BoLA
ᆶ DQB ୷ ύ DRB3
୷Ӣȑϐӭᄊ
୷ӢՉ
m, RFLPȑǵT single-stran ᄊϩȐse ݚȐdenaturi
eras chain re ۓ ׇ ϩ ࠠ ݤ
eukocyte ant
ϐǴ A୷ӢǴՠ ௨ӈຝǴ ୱᆶ IIb
ȐTakeshi Rᆶ DQ ୷ ǵDMAǵDM
ᅿаϐ D
DQ ౢނ ǴЪԜख़ᙟ A୷ӢТࢤ
୷ӢǶΟঁ
3 ୷ӢӢӭ ᄊ܄׳ࣁᡉ
Ǵӵज़ڋ Tಒझࠠᄊ nd conform
equence-sp ing gradien reaction, PC ݤ ϐ PCR
tigen, Տܭ ՠϝԖ Ꮴठ
ୱǴ ma et
୷ӢǴ MBǵ
DRᆶ ނǶӧ ᙟϐ୷ ࢤх֖
DRB ӭᄊ܄
ᡉǶ ڋТࢤ ᄊǵೈ
mation ecific nt gel CRȑǴ R-SBT
Ȑsequence ٠ࡌҥΑ
ຬၸ 110 ᅿ virus, BLV ȐJuliarena Zanotti et a
BoLA-DRB ࣁளډ PCRౢނ
ᏊϷሺᏔሽ ϕံϐТࢤ ำύௗ d ЗܭҺਡ ᆄஒࣁ ddA ݚϩᚆόӕ ddNTPǴന ϐଯှࡋ ޑ୷Ӣࠠᄊ ҁࣴز
ྍǴӆҗ
׆Ꮗૈࡌҥ
ࠠჹܭ٢ໆ
e-based typi
ӭ୷Ӣࠠ
ᅿ୷ӢࠠǴЪ Vȑǵ૫ੰ
a et al., 2008 al., 1996ȑǶ
3୷Ӣϐ P ډၨځдБݤ
ՉۓׇǴа ሽҭ࣬ჹ ࢤǴ٠ஒ dd
ddNTP ਔߡ ਡ㧿ለՏ
ATPǵddTT ӕελϐ PC നࡕҗۓׇ೬ ࡋ܄Ǵࡺё ᄊǶ
زϐҞޑࣁ
җ PCR-SBT ҥၨࣁߡճ ໆᆶ٢ಔԋ
ingȑǶMilti Ǵуၸѐ Ъϩ୷Ӣ ( dermato 8; Kulberg e
PCRۓׇϩ ݤଯϐှ
аճࡕុҔ
ܳǶ२Ӄ dNTP షӝ ߡ٬ϕံ
ǴࡺճҔԜ TPǵddGTP
CR Тࢤࡕ ೬ᡏղ᠐ࡕ ё᠘ۓۓ
ၸߚߟΕ T ϐϩݤ ϐ௦ኬБԄ ϩϐቹៜǶ
iadou et al.
ѐځдࣴزϐ Ӣࠠς
ophilosis )ᆶ et al., 2007
ϩࠠݤচϟ
ࡋǴPCR-S Ҕܭჹ୷Ӣࠠ
ӃǴճҔᆫӝ ӝܭ dNTP ύ
Тࢤคݤᝩ Ԝচᆫ
ǴаϷ ddC ࡕǴճ
ࡕӝрֹ
ۓ୷Ӣ০
ΕԄ௦ኬϐБ ݤଞჹ BoLA
Ԅаճ҂ٰ
Ƕ
2003ճҔ ϐวǴҞ
ܴᆶФқՈ ᆶᖏ܄٢
; Maillard e
ϟಏ SBTΏޔௗ
ࠠղۓा
ӝ䁙Ȑpolym ύǴၸЇ ᝩុፄᇙԶ ӝ䁙ᘉቚࡕ CTPځύ ճҔሺᏔෳ
ϐኳ݈ਡ
Տ୷Ӣϐ
БԄǴճҔ A-DRB3.2 εೕኳ၂ᡍ
ணԄᆫӝ䁙 Ҟςܭ DR
ՈੰੰࢥȐb ٢܊ݹϐל܄
et al., 1996;
ௗଞჹ BoLA
ࣗଯϐᒪ
meraseȑᘉቚ ηՉᘉቚ
ԋಖЗǴ ࡕ܌ளϐ PC ύϐǶၸ
ෳໆςՉό ਡ㧿ለׇӈ ϐӭᄊ܄Ǵ܈
Ҕ٢Фϐғ٢
୷Ӣϐӭᄊ ᡍϐ୷ᘵǴ٠
䁙ೱᙹϸᔈ RB3.2୷Ӣ bovine leuk
܄܈ܰག܄
; Xu et al.,
A-DRB3୷
ࣴزǴ
ቚрᆶኳ݈
ቚǹѿᘉ Ǵ٬ځ 3’ᆄ CR ТࢤǴ ၸଯှࡋ όӕᑻӀ
Ƕҗܭۓׇ
܈פ
٢ࣁ DN ᄊ܄Չ
٠όӕ ᔈϩ
Ӣว
kemia
܄࣬ᜢ 1993;
୷Ӣϐ
ځ၂ DNA ᘉቚၸ ᆄёಖ ځ 3’
ࡋϐႝ
ۓϐ
ׇϩ
פ൨ཥ
NA ٰ
ፕǴ ӕ୷Ӣ
