The aim of this study was to investigate whether the feasibility ofenzyme optical detection system could rapidly examine the Escherichia coli (E. coli) in food. The prototyping detection platform was built by integration of silicon photodiode, optic selective filter, photocurrent amplifier and digitize device. We make use of the β-glucuronidase (GUD) of particularity enzyme of E. coli, meeting will 4-methylumbelliferyl-β- glucuronide (MUG) resolve, producing 7-hydroxy-4- methylumbelliferyl outcome, and its outcome make use of the wave-length 366 nm to stir up the blue fluorescence that will produce the wave-length 449 nm.
The production of blue fluorescence in the enzyme optical detection system was induced by the reaction between GUD of the E. coli and MUG. We can know GUD existence that the enzyme optical detection system detects the wave-length 449 nm the fluorescence, by this existence which examines E. coli.
Furthermore, we used of Eosin Methylene Blue Agar (EMB) media and the sandwich ELISA method again to authenticate the E. coli that we separate.
Detection of E. coli in the diet meat results in both enzyme optical detection system and traditional methods. However, detection with enzyme optical detection system takes only 12 hours while the traditional method needs 5 to 7 days. It’s no doubt that the enzyme optical detection system is provided with advantage of the efficiency and automation then that can be a traditional standard method used in detecting E. coli in food.
Develop enzyme optical detection system to detect, can avoid an artificial subjective and last error margin and shorten time of examination, so fast and examine E. coli accurately, provide the food the safe and better guarantee.
