I. 四黃斑蛾擬寄生性天敵記錄共10 種,實際上會穩定寄生四黃斑 蛾的種類只有Hyposoter distriangulum Chen, Huang & Hsu、
Braconidae sp.1 及 Tachinidae sp.1 3 種。行為及生活史資訊有待 進一步的觀察與研究。最適合作為生物防治者為Braconidae sp.1,因其寄生率與豐度最高。
II. 經統計後,四黃斑蛾總寄生率為 55.03 ± 8.4%,而幼蟲死亡率不 高且羽化率高,推測只靠擬寄生性天敵無法導致其稀有性,應 有其他因素共同影響。
III. 寄主幼蟲群體數量與不同寄主植物對擬寄生性天敵總寄生率、
繭蜂寄生率及機蜂寄生率皆不影響,而不同寄主植物對寄生蠅 寄生率有顯著影響,寄主幼蟲群體數量則沒有影響。
IV. 不同寄主植物之寄生蠅寄生率與推測月桃假葉鞘能使寄主幼蟲 躲避擬寄生性天敵之假設相反,月桃族群之寄生率顯著高於三 奈族群。可能與食用月桃的族群皆為梅嶺族群,食用三奈的族 群多為崁頭山族群有關。
V. 當寄主幼蟲群體數量越多,繭蜂單隻寄主鑽出的繭數量越少,
性別偏向為male-biased 的機率增加,推測繭蜂產卵時會依寄主 數量而調整單隻寄主的產卵量與性別偏向。
21
VI. 本種蟲巢提供的保護作用對擬寄生性天敵效果有限,因此切葉 的機制與原因值得探討與研究。
VII. 以上結果有賴進行操作性實驗增進結果可信度,但必須先找出 適合四黃斑蛾交配並產卵的條件。
22
七、參考文獻
Askew, R. R. (1961). On the biology of the inhabitants of oak galls of Cynipidae (Hymenoptera) in Britain. Transactions of the Society for British Entomology, 14, 237-268.
Askew, R. R. (1971). Parasitic Insects. London: Heinemann Educ.
Books. p.316.
Chen, Y.-F., Huang, C.-L. & Hsu, Y.-F. (2017) DNA barcoding and morphological data reveal a new Hyposoter (Hymenoptera:
Ichneumonidae: Porizontinae) reared from a rare zygaenid moth Artona flavipuncta Hampson, 1900 in Taiwan. Zootaxa, 4337, 279-287.
Chaudhary, A., Bala, K., Thakur, S., Kamboj, R. & Dumra, N. (2018) Plant defenses against herbivorous insects: A Review. International Journal of Communication Systems, 6, 681-688.
Cochard, P., Galstian, T. & Cloutier, C. (2019) The influence of light environment on host colour preference in a parasitoid wasp.
Ecological entomology, 44, 105-117.
Corbett, A. & Rosenheim, J. A. (1996) Impact of a natural enemy overwintering refuge and its interaction with the surrounding landscape. Ecological Entomology, 21, 155-164.
Flanders, S. E. (1947). Elements of host discovery exemplified by parasitic Hymenoptera. Ecology, 28, 299-309.
Fisher, R. C. (1961). A Study in Insect Multiparasitism: I. Host Selection and Oviposition. Journal of Experimental Biology, 38 (2), 267–275.
Foottit, R. G. & Adler, P. H. (2009). Insect Biodiversity: Science and
23
Society. Hoboken: Wiley-Blackwell. 1, 656.
Gauld, I. & Bolton, B. (1988) The Hymenoptera. New York: British Museum (Natural History) Oxford Unersity Press. 9-29.
Gross, P. (1993) Insect behavioral and morphological defenses against parasitoids. Annual Review of Entomology, 38, 251-273.
Gullan, P. J. & Cranston, P. S. (2014). The Insects: An Outline of Entomology (5th ed.). Hoboken: Wiley-Blackwell. 362–370.
Hampson, G.F. (1900) The Moths of India: Supplementary Paper to the Volumes in“The Fauna of British India”. The journal of the Bombay Natural History Society, 13, 223-227.
Hassell, M.P. (2000) Host-parasitoid population dynamics. Journal of Animal Ecology, 69, 543-566.
Hedrick, P. W. (1984) Population Biology. Portola Valley: Jones and Bartlett Publishers.
Holling, C.S. (1961) Principles of insect predation. Annual Review of Entomology, 6, 163-182.
Howard, L. O. & Fiske, W. F. (1911). The importation into the United States of the parasites of the gipsy moth and the brown-tail moth, Bull. U.S. Bur. Ent. 91, 312.
Jervis, M. A., Heimpel, G. E., Ferns, P. N., Harvey, J. A. & Kidd, N. A.
(2001) Life‐history strategies in parasitoid wasps: a comparative analysis of ‘ovigeny’. Journal of Animal Ecology, 70, 442-458.
Jervis, M. A., Ellers, J., Harvey, J. A. (2008) Resource acquisition, allocation, and utilization in parasitoid reproductive strategies.
Annual Review of Entomology, 53, 361-385.
24
Kawamata, R., Sato, Y., Suzuki, M. & Kainoh, Y. (2018) Color preference and associative color learning in a parasitoid wasp, Ascogaster reticulata (Hymenoptera: Braconidae). Journal of Insect Behavior, 31, 523-534.
Lanham, U. (1964) The insects. New York: Columbia University Press.
120-125, 235-241.
Libersat, F., Delago, A. & Gal, R. (2009) Manipulation of host behavior by parasitic insects and insect parasites. Annual Review of
Entomology, 54, 189-207.
Reuter, O. M. (1913) Lebensgewohnheiten und Instinkte den Inseckten bis zum Erwachen der Sozialoen Instinkte. Berlin: Friedlander. 448.
Richards, O.W. (1940). The biology of the small white butterfly (Pieris rapae), with special reference to the factors controlling its
abundance. J. Anim. Ecol., 9, 243-88.
Richards, O.W. (1977) Hymenoptera Introduction and Key to Families, Second edition. London: Royal Entomological Society of London.
Smith, H. S. (1935). The role of biotic factors in the determination of population densities.Journal of Economic Entomology, 28, 873-98.
Smith, H. S. (1937). Review: Sweetman, H. L. (1936) The biological control of insects. Journal of Economic Entomology, 30, 218-20.
Solomon, M.E. (1949) The Natural Control of Animal Populations.
Journal of Animal Ecology, 18, No.1, 1-35.
Stamp, N. E. (1982) Behavioral Interactions of Parasitoids and Baltimore Checkerspot Caterpillars (Euphydryas phaeton). Evironmental entomology, 11, 100-104.
25
Thompson, W. R. (1928). A contribution to the study of biological control and parasitic introduction in con- tinental areas. Parasitology, 20, 90-112.
Vinson, S. B. (1976) Host selection by insect parasitoids. Annual Review of Entomology, 21, 109-133.
Vinson, S. B. (1985) The behavior of parasitoids.Comprehensive Insect Physiology, Biochemistry and Pharmacology, 9, 417-469.
Werren, J. H. (1983) Sex ratio evolution under local mate competition in a parasitic wasp. Evolution, 37, 116-124.
Weseloh, R.M. (1973) Relationships of Natural Enemy Field Populations to Gypsy Moth Abundance. Annals of the Entomological Society of America, 66, 4, 853-856.
Wibawa, I Putu Agus Hendra. (2019) Perbandingan Efektifitas Ekstrak Legundi (Vitex trifolia L.), Sirsak (Annona muricata L.) Dan Damar (Agathis borneensis Warb.) Terhadap Mortalitas Larva Artona
flavipuncta Hama Pada Tanaman Kecombrang (Etlingera elatior (Jack) R.M.Sm.). Agro Bali, Agricultural Journal, 21-27.
張宸睿(2018):四黃斑蛾生活使與其切葉行為研究。國立臺灣師範 大學生命科學系碩士論文。
26
圖一、四黃斑蛾生活史 (A):聚產卵群;(B):刮食食痕與群聚的小 幼蟲;(C):群聚的幼蟲;(D):幼蟲躲藏在月桃之假葉鞘內;(E):
於枯葉中作繭;(F):雌性成蟲 (A)
c
(B)
(C) (D)
(E) (F)
27
圖二、四黃斑蛾幼蟲切葉行為 (A):幼蟲切葉順序,紅線為切痕,
藍色箭頭為切葉方向;(B)切痕近照;(C):葉片下垂與枯黃捲曲,
形成蟲巢
(B) (C)
(A)
28
圖三、四黃斑蛾目擊地點。 :成蟲目擊地點;::幼蟲目擊與採 集地點
29
圖四、四黃斑蛾採集地點。 :主要採集地點;::補充採集地點
30
圖五、姬蜂 (A):Hyposoter distriangulum Chen, Huang & Hsu 2017;
(B):Charops sp.;(C):Hyposoter distriangulum 的蛹;(D):
Charops sp. 的蛹 (A)
(A) (B)
(C) (D)
31
圖六、繭蜂 (A):Braconidae sp.1;(B):Braconidae sp.2;(C):繭蜂 幼蟲鑽出寄主;(D):繭蜂的繭;(E):單寄主羽化之 sp.1 性別偏向
Total n = 131 host larvae
(A) (B)
(C) (D)
(E)
♀-biased, 67.94 % n = 89
♂-biased, 22.14 % n = 29
Non-biased, 9.92 % n = 13
32
圖七、小蜂與寄生蠅 (A):Chalcidoidea sp.1;(B):Chalcidoidea sp.2;(C):Chalcidoidea sp.3;(D):Chalcidoidea sp.4;(E):
Tachinidae sp.1 (F):Tachinidae sp.2;(G):Tachinidae sp.1 的蛹
(A) (B)
(C) (D)
(E) (F)
(F)
33 Ichneumonidae, 16.15 ± 6.0 %
34
圖九、單隻寄主鑽出的繭數量和群體數量的相關性:繭數量 = - 0.040456 x 群體數量 + 3.1934475 , R2 = 0.150467 , P < 0.001
35
圖十、寄生蠅月桃與三奈族群寄生率之EM 平均值:月桃 – 三奈 = 21.58 ± 9.86%, df = 1, P < 0.05
三奈 月桃
EM平均值 3.11 24.69
95% Wald 信賴區間上限 4.14 36.65
95% Wald 信賴區間下限 2.34 16.64
0 5 10 15 20 25 30 35 40
36
37
表一、四黃斑蛾擬寄生性天敵清單與分佈,標註*者為穩定寄生的種類
Species Distribution
*Hyposoter sp. 台南市東山區崁頭山、台南市楠西區梅嶺、南投縣信義鄉和社、南投縣鹿谷鄉田底
Charops sp. 台南市東山區崁頭山
*Braconidae sp.1 台南市東山區崁頭山、台南市楠西區梅嶺、南投縣信義鄉和社、南投縣鹿谷鄉田底、嘉義縣番路鄉土匪山、
苗栗縣三義鄉關刀山、南投縣鹿谷鄉溪頭、高雄桃源鄉梅口山 Braconidae sp.2 台南市東山區崁頭山
Chalcidoidea sp.1 台南市東山區崁頭山 Chalcidoidea sp.2 台南市東山區崁頭山 Chalcidoidea sp.3 台南市東山區崁頭山
Chalcidoidea sp.4 台南市東山區崁頭山、台南市楠西區梅嶺
*Tachinidae sp.1 台南市東山區崁頭山、台南市楠西區梅嶺、南投縣信義鄉和社、嘉義縣番路鄉土匪山、高雄甲仙區白雲寺、
嘉義縣阿里山鄉山美、台中和平區斯巴可步道 Tachinidae sp.2 台南市東山區崁頭山、南投縣鹿谷鄉溪頭
38
表二、群體數量預測繭蜂性別偏向之邏輯迴歸分析,n = 124
B S.E. Wald df P OR
95% CI of OR 下限 上限 群體數量 0.056 0.025 5.019 1 0.025 1.058 1.007 1.111
常數 -1.982 0.466 18.049 1 0.000 0.138 Omnibus 檢定:x2 = 5.172, p = 0.023
39
表三、不同飼養下擬寄生性天敵成蟲存活時間
Hyposoter sp. Braconidae sp.1 Tachinidae sp.1 n Mean±SE n Mean±SE n Mean±SE 蜂蜜水 20 12.15 ± 2.03 30 8.5 ± 1.15 10 12 ± 3.37 無餵食 9 2.88 ± 0.94 28 < 1 11 3.91 ± 1.22