在台灣，每年有許多患者死於並非不治之症的慢性疾病，其中，慢性肝病就 是其中一項，由於目前所發展出的藥物皆以輔助治療為主，在缺乏對症藥物的情 況下，嚴重至肝硬化之患者往往只能等待肝臟移植。由於台灣法律對於器官捐贈 的諸多限制，以及配對資料庫的不健全，讓許多患者等不到器官移植之機會。
醫學發展日益進步的當下，許多新療法因應而生，幹細胞治療為其中一種，
雖然在科學家眼中前景看好，但因其充滿太多未知，故各國之醫療總署遲遲無法 開放幹細胞治療進入正式醫療療程。近年來，美國食品藥物管理局 (Food and Drug Administration, FDA) 核准由脂肪組織分離含有脂肪幹細胞之 stromal-vascular fraction 可用於整形手術中之自體脂肪移植，同時開放許多自體幹細胞修補手術，
如胎兒皮膚幹細胞修補新生兒兔唇、心外露、氣管缺陷等，使得自體細胞治療向 前邁進了一大步。
科學領域需要不斷的投入以發現新知，也許在不久的將來，我們不再需要人 造器官或是器官移植，能以自體幹細胞治療，修補許多具有缺陷，或是功能不佳 的器官，期待在未來，生醫科學的進步，能帶給全世界的人們幸福。
參考文獻
Baddoo, M., Hill, K., Wilkinson, R., Gaupp, D., Hughes, C., Kopen, G. C. and Phinney, D. G. 2003. Characterization of mesenchymal stem cells isolated from murine bone marrow by negative selection. Journal of cellular biochemistry 89:
1235-1249.
Banas, A., Teratani, T., Yamamoto, Y., Tokuhara, M., Takeshita, F., Quinn, G., Okochi, H. and Ochiya, T. 2007. Adipose tissue-derived mesenchymal stem cells as a source of human hepatocytes. Hepatology 46: 219-228.
Bataller, R., and D. A. Brenner. 2005. Liver fibrosis. The Journal of clinical investigation 115: 209-218.
Bianco, P., M. Riminucci, S. Gronthos, and P. G. Robey. 2001. Bone marrow stromal stem cells: nature, biology, and potential applications. Stem Cells 19: 180-192.
Bittner, R. E., Schofer, C., Weipoltshammer, K., Ivanova, S., Streubel, B., Hauser, E., Freilinger, M., Hoger, H., Elbe-Burger, A. and Wachtler, F. 1999. Recruitment of bone-marrow-derived cells by skeletal and cardiac muscle in adult dystrophic mdx mice. Anatomy and embryology 199: 391-396.
Bjorklund, L. M., Sanchez-Pernaute, R., Chung, S., Andersson, T., Chen, I. Y., McNaught, K. S., Brownell, A. L., Jenkins, B. G., Wahlestedt, C., Kim, K. S. and Isacson, O. 2002. Embryonic stem cells develop into functional dopaminergic neurons after transplantation in a Parkinson rat model. Proceedings of the National Academy of Sciences of the United States of America 99: 2344-2349.
Boeker, K. H., Haberkorn, C. I., Michels, D., Flemming, P., Manns, M. P. and Lichtinghagen, R.2002. Diagnostic potential of circulating TIMP-1 and MMP-2 as
Campagnoli, C., Roberts, I. A.,Kumar, S., Bennett, P. R., Bellantuono, I. and Fisk, N.
M. 2001. Identification of mesenchymal stem/progenitor cells in human first-trimester fetal blood, liver, and bone marrow. Blood 98: 2396-2402.
Caplan, A. I. 1991. Mesenchymal stem cells. Journal of orthopaedic research : Official Publication of the Orthopaedic Research Society 9: 641-650.
Chamberlain, G., J. Fox, B. Ashton, and J. Middleton. 2007. Concise review:
mesenchymal stem cells: their phenotype, differentiation capacity, immunological features, and potential for homing. Stem Cells 25: 2739-2749.
Chan, S. L., Choi, M., Wnendt, S., Kraus, M., Teng, E., Leong, H. F. and Merchav, S.
2007. Enhanced in vivo homing of uncultured and selectively amplified cord blood CD34+ cells by cotransplantation with cord blood-derived unrestricted somatic stem cells. Stem Cells 25: 529-536.
Chen, J., Li, Y., Wang, L., Zhang, Z., Lu, D., Lu, M. and Chopp, M. 2001. Therapeutic benefit of intravenous administration of bone marrow stromal cells after cerebral ischemia in rats. Stroke; A Journal of Cerebral Circulation 32: 1005-1011.
Di Nicola, M., Carlo-Stella, C., Magni, M., Milanesi, M., Longoni, P. D., Matteucci, P., Grisanti, S. and Gianni, A. M. 2002. Human bone marrow stromal cells suppress T-lymphocyte proliferation induced by cellular or nonspecific mitogenic stimuli.
Blood 99: 3838-3843.
Djouad, F., Plence, P., Bony, C., Tropel, P., Apparailly, F., Sany, J., Noel, D. and Jorgensen, C. 2003. Immunosuppressive effect of mesenchymal stem cells favors tumor growth in allogeneic animals. Blood 102: 3837-3844.
Dor, Y., J. Brown, O. I. Martinez, and D. A. Melton. 2004. Adult pancreatic beta-cells are formed by self-duplication rather than stem-cell differentiation. Nature 429:
41-46.
Evans, M. J., and M. H. Kaufman. 1981. Establishment in culture of pluripotential cells from mouse embryos. Nature 292: 154-156.
Fang, D., Seo, B. M., Liu, Y., Sonoyama, W., Yamaza, T., Zhang, C., Wang, S. and Shi, S. 2007. Transplantation of mesenchymal stem cells is an optimal approach for plastic surgery. Stem Cells 25: 1021-1028.
Hoehn, M., Kustermann, E., Blunk, J., Wiedermann, D., Trapp, T., Wecker, S., Focking, M., Arnold, H., Hescheler, J., Fleischmann, B. K., Schwindt, W. and Buhrle, C.
2002. Monitoring of implanted stem cell migration in vivo: a highly resolved in vivo magnetic resonance imaging investigation of experimental stroke in rat.
Proceedings of the National Academy of Sciences of the United States of America 99: 16267-16272.
Hong, S. H., Gang, E. J., Jeong, J. A., Ahn, C., Hwang, S. H., Yang, I. H., Park, H. K., Han, H. and Kim, H. 2005. In vitro differentiation of human umbilical cord blood-derived mesenchymal stem cells into hepatocyte-like cells. Biochemical and Biophysical Research Communications 330: 1153-1161.
In 't Anker, P. S., Scherjon, S. A., Kleijburg-van der Keur, C., Noort, W. A., Claas, F.
H., Willemze, R., Fibbe, W. E. and Kanhai, H. H. 2003. Amniotic fluid as a novel source of mesenchymal stem cells for therapeutic transplantation. Blood 102:
1548-1549.
Koc, O. N., Gerson, S. L., Cooper, B. W., Dyhouse, S. M., Haynesworth, S. E., Caplan, A. I. and Lazarus, H. M. 2000. Rapid hematopoietic recovery after coinfusion of autologous-blood stem cells and culture-expanded marrow mesenchymal stem cells in advanced breast cancer patients receiving high-dose chemotherapy.
Lee, K. D., Kuo, T. K., Whang-Peng, J., Chung, Y. F., Lin, C. T., Chou, S. H., Chen, J.
R., Chen, Y. P. and Lee, O. K. 2004. In vitro hepatic differentiation of human mesenchymal stem cells. Hepatology 40: 1275-1284.
Menon, L. G., Picinich, S., Koneru, R., Gao, H., Lin, S. Y., Koneru, M., Mayer-Kuckuk, P., Glod, J. and Banerjee, D. 2007. Differential gene expression associated with migration of mesenchymal stem cells to conditioned medium from tumor cells or bone marrow cells. Stem Cells 25: 520-528.
Nakagami, H., Morishita, R., Maeda, K., Kikuchi, Y., Ogihara, T. and Kaneda, Y. 2006.
Adipose tissue-derived stromal cells as a novel option for regenerative cell therapy.
Journal of Atherosclerosis and Thrombosis 13: 77-81.
Parviz, F., Matullo, C., Garrison, W. D., Savatski, L., Adamson, J. W., Ning, G., Kaestner, K. H., Rossi, J. M., Zaret, K. S. and Duncan, S. A. 2003. Hepatocyte nuclear factor 4alpha controls the development of a hepatic epithelium and liver morphogenesis. Nature Genetics 34: 292-296.
Peters, T., Jr. 1985. Serum albumin. Advances in protein chemistry 37: 161-245.
Phinney, D. G., G. Kopen, R. L. Isaacson, and D. J. Prockop. 1999. Plastic adherent stromal cells from the bone marrow of commonly used strains of inbred mice:
variations in yield, growth, and differentiation. Journal of Cellular Biochemistry 72: 570-585.
Pittenger, M. F., Mackay, A. M., Beck, S. C., Jaiswal, R. K., Douglas, R., Mosca, J. D., Moorman, M. A., Simonetti, D. W., Craig, S. and Marshak, D. R. 1999.
Multilineage potential of adult human mesenchymal stem cells. Science 284:
143-147.
Recknagel, R. O. 1967. Carbon tetrachloride hepatotoxicity. Pharmacological Reviews 19: 145-208.
Ruhnke, M., Ungefroren, H., Zehle, G., Bader, M., Kremer, B. and Fandrich, F. 2003.
Long-term culture and differentiation of rat embryonic stem cell-like cells into neuronal, glial, endothelial, and hepatic lineages. Stem Cells 21: 428-436.
Saito, T., J. E. Dennis, D. P. Lennon, R. G. Young, and A. I. Caplan. 1995. Myogenic Expression of Mesenchymal Stem Cells within Myotubes of mdx Mice in Vitro and in Vivo. Tissue Engineering 1: 327-343.
Sakaida, I., Terai, S., Yamamoto, N., Aoyama, K., Ishikawa, T., Nishina, H. and Okita, K. 2004. Transplantation of bone marrow cells reduces CCl4-induced liver fibrosis in mice. Hepatology 40: 1304-1311.
Sanchez-Ramos, L., and A. M. Kaunitz. 2000. Misoprostol for cervical ripening and labor induction: a systematic review of the literature. Clinical Obstetrics and Gynecology 43: 475-488.
Schaffler, A., and C. Buchler. 2007. Concise review: adipose tissue-derived stromal cells--basic and clinical implications for novel cell-based therapies. Stem Cells 25:
818-827.
Schmidt, C., Bladt, F., Goedecke, S., Brinkmann, V., Zschiesche, W., Sharpe, M., Gherardi, E. and Birchmeier, C. 1995. Scatter factor/hepatocyte growth factor is essential for liver development. Nature 373: 699-702.
Schwartz, R. E., Reyes, M., Koodie, L., Jiang, Y., Blackstad, M., Lund, T., Lenvik, T., Johnson, S., Hu, W. S. and Verfaillie, C. M. 2002. Multipotent adult progenitor cells from bone marrow differentiate into functional hepatocyte-like cells. The Journal of Clinical Investigation 109: 1291-1302.
Sgodda, M., Aurich, H., Kleist, S., Aurich, I., Konig, S., Dollinger, M. M., Fleig, W. E.
2875-2886.
Sheweita, S. A., M. Abd El-Gabar, and M. Bastawy. 2001. Carbon tetrachloride-induced changes in the activity of phase II drug-metabolizing enzyme in the liver of male rats: role of antioxidants. Toxicology 165: 217-224.
Slater, T. F., and B. C. Sawyer. 1971. The stimulatory effects of carbon tetrachloride on peroxidative reactions in rat liver fractions in vitro. Inhibitory effects of free-radical scavengers and other agents. The Biochemical Journal 123: 823-828.
Sun, Y., Chen, L., Hou, X. G., Hou, W. K., Dong, J. J., Sun, L., Tang, K. X., Wang, B., Song, J., Li, H. and Wang, K. X. 2007. Differentiation of bone marrow-derived mesenchymal stem cells from diabetic patients into insulin-producing cells in vitro. Chinese Medical Journal 120: 771-776.
Takahara, T., Furui, K., Yata, Y., Jin, B., Zhang, L. P., Nambu, S., Sato, H., Seiki, M.
and Watanabe, A. 1997. Dual expression of matrix metalloproteinase-2 and membrane-type 1-matrix metalloproteinase in fibrotic human livers. Hepatology 26: 1521-1529.
Thomson, J. A. , Itskovitz-Eldor, J., Shapiro, S. S., Waknitz, M. A., Swiergiel, J. J., Marshall, V. S. and Jones, J. M. 1998. Embryonic stem cell lines derived from human blastocysts. Science 282: 1145-1147.
Tropel, P., Noel, D., Platet, N., Legrand, P., Benabid, A. L. and Berger, F. 2004.
Isolation and characterisation of mesenchymal stem cells from adult mouse bone marrow. Experimental Cell Research 295: 395-406.
Weber, L. W., M. Boll, and A. Stampfl. 2003. Hepatotoxicity and mechanism of action of haloalkanes: carbon tetrachloride as a toxicological model. Critical Reviews in Toxicology 33: 105-136.
Westin, J., L. M. Lagging, R. Wejstal, G. Norkrans, and A. P. Dhillon. 1999.
Interobserver study of liver histopathology using the Ishak score in patients with chronic hepatitis C virus infection. Liver 19: 183-187.
Zhao, D. C., Lei, J. X., Chen, R., Yu, W. H., Zhang, X. M., Li, S. N. and Xiang, P. 2005.
Bone marrow-derived mesenchymal stem cells protect against experimental liver fibrosis in rats. World Journal of Gastroenterology : WJG 11: 3431-3440.
Zuk, P. A., Zhu, M., Ashjian, P., De Ugarte, D. A., Huang, J. I., Mizuno, H., Alfonso, Z.
C., Fraser, J. K., Benhaim, P. and Hedrick, M. H. 2002. Human adipose tissue is a source of multipotent stem cells. Molecular Biology of the Cell 13: 4279-4295.