為確認本研究之毛細管區帶電泳紫外光偵測法(CZE-UV)對 SCFAs 分析的有效性，因此透過實際樣品，使用 Zhao 等學者(2006)發表的氣 相層析儀/火焰離子化偵測器方法(GC-FID) 進行分析比對，結果列於 表 4-9 中。
首先就分析能力比較， GC-FID 具有偵測極限較佳的優點，但再 現性、檢量線性範圍則和 CZE-UV 並無明顯差異，而回收率方面，
GC-FID 為 87.7%–109.7%，CZE-UV 為 100.1%–102.2%，以 CZE-UV 表現較佳，推測其原因在於 GC-FID 方法中使用特殊管柱會造成有機 酸殘留所致(Zhao et al., 2006)。
接著就操作效率比較，GC-FID 使用特殊管柱能夠與 CZE-UV 一 樣，不須經繁雜的樣品衍生化處理而直接分析，但缺點是有機酸易在 特殊管柱內殘留，連續分析時會有鬼峰(ghost peak)產生的現象，因此 需定時以甲酸沖洗來解決 (Zhao et al., 2006)。這問題可能是 GC-FID 回收率變異較大的原因，同時增加操作複雜性以及化學試劑需求。另 一方面，GC-FID 與 CZE-UV 之 SCFAs 分析滯留時間雖然皆相當迅 速，僅約需 10 分鐘，但從整體的分析時間而言，CZE-UV 則明顯佔 優勢，因為當所有 SCFAs 檢測出後，CZE-UV 可立刻以電解溶液藉由 壓力直接將其他殘留物質完全洗出，相對地，GC-FID 就只能經由改 變管柱溫度條件加快冲提速度，自待殘留物質完全流出管柱，使整體
最後就經濟成本考量， GC-FID 需要專用分析管柱，以及高純度 之氦氣和氫氣，但 CZE-UV 則只需相當便宜的空毛細管柱，分析所須 的電解液量相當節省，明顯具有低成本優勢。
伍、 結論
4. 於最適條件下，樣品添加 1 mM 2-ethylbutyric acid 作為內標準品，可於 十分鐘內同時定量包括乙酸、丙酸、丁酸、異丁酸和戊酸等五種短鏈脂
和 GC-FID 相當。因此可取代 GC-FID 作為乳製品中短鏈脂肪酸之分析 方法，以降低成本，提高效能。
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表 4.1 毛細管電泳分析 SCFAs 的文獻
Table 4.1 The application of capillary electrophoresis in the analysis of SCFAs
Organic acids Matrix Running electrolyte Detection Reference
acetic, propionic, butyric, i-butyric, valeric
Chyme of swine, Dairy products
15 mM benzoate, 0.5 mM CTAB, pH 11.0 Indirect UV detection at 226 nm
本論文研究
β -hydroxybutyric, acetic, lactic, glycolic, propionic , butyric, formic
Atmospheric particulate mat ter
10 mM 3, 5-dinit roben-acid, 0. 1 mM CTAB, pH 5－6
Indirect UV detection at 254 nm
Souza et al.
1998 formic, fumaric,
propionic , oxalic, malic, tartaric, acetic, lactic, cit ric
Formula feed 20 mM PDC, 0.5 mM HTAOH, pH 5.7
Indirect UV detection at 375 nm
Ishikuro et al.
2000 oxalic, formic, acetic,
propionic, butyric
Riverwater 5 mM chromate, 4 % n-butanol , 0. 1 mM O FM (Qc) –anion-B T, pH 8. 0.
Indirect UV detection at 254 nm
Forray and Hallbauer et al.
2000 oxalic, formic, malonic,
fumaric, succinic, citric, acetic , maleic, tartaric, glutaric, adipic, propionic, butyric, valeric hydroxide, pH 8.5
Indirect UV detection at 254 nm
Hagberg et al.
2000
oxalate, sulphurate, formate, lactata, acetate, propanoate
Air sample 5 mM PDC, 0.5 mM CT AB,pH 5.6 Indirect UV detection at 200 nm
Levart et al.
2000 gluconic, acetic Milk 10 mM benzoate ,1 mM C TAB, pH 5. 4 Indirect UV detection at
220 nm
Ehala et al.
2001 formic, malonic, succinic,
2001 formic, malonic, succinic,