根據《第三章 蝴蝶蘭於養分逆境下的生長反應》的試驗結果得知,蝴蝶蘭對 於缺氮、缺磷及缺鉀逆境具有高的耐受性。在缺肥處理 8 週以前,蝴蝶蘭的外觀 與生理參數包括葉長、葉面積、鮮重與乾重、光合作用能力皆不受缺氮、缺磷或 缺鉀處理影響,僅同時缺乏三大元素之處理(-NPK)的葉片生長與重量稍微下降;
當缺肥處理 12 週後,缺磷植株的第一片成熟葉的葉片長度與面積、植株鮮重與乾 重皆不受影響,與對照組沒有差異,另外,即使缺氮已經可以由葉綠素計讀值監 測到 SPAD 值下降,但在外觀上無法用肉眼比較出缺氮、缺磷或缺鉀 12 週對植株 的影響,植株健壯、且葉色濃綠。由此結果顯示蝴蝶蘭可以耐受長達 3 個月的缺 肥,不易由外觀看出徵狀。
將蝴蝶蘭分別置於不同溫度下而維持營養生長或促使轉為生殖生長,同樣地 處理缺氮、缺磷或缺鉀 32 週,經低溫誘導開花的植株其缺肥徵狀較明顯,顯示花 梗與花朵為生殖生長期間的強積儲,會消耗大量養分而造成植株生長量與開花表 現受到影響。缺氮造成開花表現不佳,花朵減少、變小且花梗縮短,缺磷會抑制 植株的抽梗現象,而缺鉀處理的植株在本試驗中,外觀與生理參數包括光合作用 能力皆不受影響,僅抽梗時間延後。由以上結果顯示,蝴蝶蘭的開花過程需要充 足的氮與磷,而本試驗的植株可能在處理前已貯存足夠的鉀,因此缺鉀徵狀不明 顯。營養生長下的植株在長期的缺氮、缺磷、缺鉀逆境下 32 週後,缺氮與同時缺 乏氮、磷、鉀(-NPK)處理的植株生長勢與鮮重明顯下降,外觀生長不良;缺磷雖 然第一片成熟葉的生長不受影響,但有明顯的落葉現象;缺鉀的植株則沒有任何 徵狀。綜合以上的生理調查結果顯示,蝴蝶蘭缺乏元素的徵狀發生情形很緩慢,
其中必定有相關的生理機制使之能克服長期的缺肥逆境。
《第四章 蝴蝶蘭於缺磷下的形態解剖觀察》的試驗結果中,缺磷 22-23 週對
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於蝴蝶蘭葉片與根部的細胞組織結構層面上改變不顯著,缺磷的葉片橫切面與對 照組無差異,但是缺磷植株的根部中,中柱面積與維管束數目顯著少於對照組,
並以距離根尖 1 公分之橫切面最顯著,推論缺磷可能造成根部的生長與發育受阻、
減緩。
在《第五章 蝴蝶蘭於缺磷下的基因表現》的試驗中,嘗試以基因分子層面進 一步找出受到缺磷誘導反應的基因。首先,以 RT-PCR 方法驗證微矩陣結果中的候 選基因,結果顯示,目前只有在葉片中大量上調表現的基因可以被驗證,有 10 個 候選基因在本試驗中可偵測到其受缺磷而誘導增加表現量,其中以蝴蝶蘭基因 PATC128122 表現量與對照組差異最大,且不受缺氮、缺鉀影響。因此現階段中,
將 PATC128122 大量表現於阿拉伯芥中,嘗試探討此基因的功能,但是阿拉伯芥
35S::PATC128122 轉殖株於缺磷條件下與野生株無差異,另外以 RNA 原位雜交方
式偵測基因 PATC128122 於蝴蝶蘭葉片與根部中的表現位置,得知主要表現於葉片 的韌皮部與根部的皮層上。
蝴蝶蘭基因 PATC128122 的蛋白質序列上具有一段 RING domain,可能與許多 生理代謝的調控路徑相關,雖然目前仍不知道其功能性,但可確定此為蝴蝶蘭於 缺磷逆境下會大量表現的代表基因。研究蝴蝶蘭的基因調控的研究與技術仍少,
目前雖然大量表現 PATC128122 於阿拉伯芥中不能看出差異,但可利用其他方法再 進行測試,例如以病毒誘導基因沉默(virus induced gene silencing, VIGS)技術等。未 來可再挑選其他於缺磷下誘導反應的候選基因,例如 PATC154300、PATC155606 或其他具有功能性的轉運蛋白、轉錄因子,進一步地研究蝴蝶蘭面臨缺磷逆境的 分子調控機制。
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