補充八週綠茶萃取物後，經單次 70% V‧
O2 peak固定式腳踏車運動 60 分鐘後的
恢復期，立刻補充碳水化合物飲食，結果發現呼吸交換率降低，游離脂肪酸提高，
肌肉肝醣合成提高。
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附錄五 肌肉穿刺步驟
1、在人體右側大腿找到股外側肌肉(vastus lateralis)，並做記號。
2、於記號處使用優碘與酒精各消毒三次。
3、鋪蓋已殺菌之洞巾。
4、注射 1-2 毫升局部麻醉藥 (2% Lignocaine)，內含血管收縮素防止出血。
5、使用 14 號肌肉穿刺針取得股外側肌肉樣本，取樣過程非常迅速，用一次即丟 棄。
6、取出穿刺針後，局部再用優碘消毒，並用紗布壓迫止血，受試者休息 30 分鐘 防止出血。若還有疼痛，再加以冰敷。
7、為防感染，受試者 2 天內禁止傷口碰水，並且每天更換紗布。
8、為防傷口再出血，三天內禁止運動，一星期內禁止激烈運動。
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5、室溫下加入 250μl 的 0.3M sodium acetate buffer，pH＝4.8，vortex 均勻。
6、立即調配冰醋酸水溶液 (glacial acetic acid : ddH2O = 1 : 1) 置於小管中，小管 需用錫箔紙包起避光；加入 10μl 冰醋酸水溶液於肌肉樣本溶液中，vortex。
7、加入 250μl Amyloglucosidase (Amyloglucosidase in 0.3M sodium acetate buffer, 10mg/ml, pH＝4.8)，vortex。
8、室溫下靜置至少 12 小時。此時可先 label 好隔天要用的小管 (2 ml)。
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11、肌肉樣本溶液取 150μl 各裝成 2 小管。
12、加入 1.5ml 呈色劑 (glucose color reagent，Trinder)，注意時間間隔，約每 30 秒加一管，才可控制每管的反應時間相同，vortex 均勻。
13、室溫下靜置 21 分鐘，第 21 分鐘使用分光光度計 Beckman DU 650
UV-Spectrophotometer(Beckman Coulter, Inc., Fullerton, CA) 檢測，波長定為 505nm。
14、輸出資料，進行資料分析。