潛變的特性，所以隨著負載加大、時間增長會導致應力開裂的情 形，因此材料表面會有破損現象的產生。
3. 持壓時間的長短對於熱壓印材料也有顯著的影響，當溫度較低且 持壓時間過短時，容易造成試片表面壓印不完整，所以表面很明 顯的可以看出干涉條紋分布不均勻的現象。當溫度較高且持壓時 間過長時，容易使材料因為長時間高溫環境下，造成材料試片表 面開始產生小氣泡與軟化變形的現象，因此溫度較低時需使用較 長的壓印時間，溫度較高時持壓時間應該縮短，如此才能得到較 好的壓印結果。
4. U 型溝槽試片所培養的細胞在貼附的階段就有規律性的貼附排列 現象產生，接著分化階段，細胞並不會像正常肌肉細胞一樣如樹 枝狀的分化，而是同方向性的分化，到後期細胞成形階段，細胞 一樣順著溝槽方向排列生長。而培養於V 型溝槽試片的細胞卻沒 有同方向貼附排列的現象，其生長情況和一般正常的成肌細胞相 似。這主要是因為U 型溝槽的深度較深，所以深寬比大，因此能 有效的誘導細胞做同方向性的貼附排列，另一原因在於轉印後的 材料試片，由於V 型溝槽試片其溝槽凸起寬度變得十分細窄，因 此沒有足夠的空間能讓細胞的觸角去碰觸與移動，導致不能有效 的誘導細胞成同方向性的生長排列。
5. 利用電漿表面改質來改變材料表面的親水性，由實驗觀察可知，
經過氬電漿處理過後的U 型溝槽試片細胞貼附效果比較好，且細 胞有同方向性排列的現象，但是經由氧電漿處理過後的試片，細 胞已經沒有同方向性排列的現象，此原因在於氬氣為惰性氣體，
能避免材料表面容易與外界起化學反應，進而破壞材料表面微結 構，影響細胞貼附與排列的行為。另外，PMMA 材料經電漿改質
增加表面親水性，其貼附效果並沒有培養皿底盤的PS 材料來的穩 定，由此可知，PMMA 材料經電漿表面改質後，其表面親水性似 乎對於細胞貼附的效果並沒有很太大的改善。
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