• 沒有找到結果。

本研究使用在離子液體BMIM-PF6的分布大於水相的染料

Procion Blue MX-R當作萃取劑,可對與其有親和力作用的溶菌酶進行 正向及反向萃取。當離子液體相中的親和性染料濃度愈高時,對蛋白 質的萃取效果隨之提升。此方法與傳統雙水相系統相較之下,由於染 料配體省略了在高分子聚合物上進行修飾(modification)的步驟,因 此較為簡易;同時使用離子液體取代傳統有機溶劑作為萃取相,減少 化學溶劑的揮發,降低了對環境的影響。

本實驗同時測試了Procion Blue MX-R 對其他種類蛋白質是否具 有萃取效果,結果發現,當分別萃取各個蛋白質水溶液時,僅溶菌酶 可被成功萃取,可知本實驗所選擇的染料與蛋白質的作用力具有一定 程度的選擇性。

由於親和性染料和離子液體的種類繁多,選擇適當的染料與疏水 性離子液體,預期能萃取的蛋白質種類將大為增加,為離子液體應用 在蛋白質萃取方面,提供了新的研究方向。

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