我們成功的將PAA修飾到SiO2表面，並以其對lysozyme進行吸附 脫附達到濃縮效果。於SiO2@PAA最佳合成條件的研究上，我們以微 波加熱方式將傳統加熱所需的24小時反應時間大幅縮小到10分鐘。由 IR、EA和TGA的實驗結果顯示，在傳統加熱法下，以較大的SiO2顆 粒(200 μm)與分子量較大的PAA(Mw 250000)反應，所合成出的
SiO2@PAA具有較大的修飾量。根據EA與TGA的結果推論，在200 μm 的SiO2上每1莫耳的SiO2可以修飾上0.1莫耳的APTMS，而
SiO2@APTMS上每莫耳的APTMS則可與0.01莫耳分子量250000的 PAA鍵結成為SiO2@PAA。在微波加熱法方面，雖然在合成
SiO2@APTMS和SiO2@PAA時能有效的縮短合成所需時間，以及較傳 統加熱法多一倍的APTMS修飾量，然而SiO2@APTMS與PAA反應生 成SiO2@PAA的修飾量卻不如傳統加熱方式。推斷微波加熱的特有加 熱方式造成PAA自行聚合等副反應的進行，使得只有少量的PAA與 APTMS進行縮合反應。
以SiO2@PAA顆粒對lysozyme進行吸脫附實驗的結果顯示，PAA 的修飾量如預期地與lysozyme吸附量有直接的關係。此外SiO2@PAA 對lysozyme的吸附與脫附量會受到溶液pH值、鹽類濃度與吸附脫附時 間的影響。於所探討的條件之中發現，控制溶液條件在pH8.5、無鹽
類濃度的環境下，SiO2@PAA可以較SiO2多了7倍的吸附量。若以濃縮 效果而言，SiO2@PAA顆粒對100 ppm lysozyme溶液至少有15倍以上 的濃縮效果。
離子強度的調控則方便lysozyme由SiO2@PAA脫附出的最佳方 式，但即使在1 M NaCl的離子強度下也只能有約70%的lysozyme由 SiO2@PAA脫附出，雖然有文獻報導於更高的離子強度下可將蛋白質 現有直接以長碳鏈的PAA分子與SiO2@APTMS進行縮合反應。由於
PAA高分子間彼此立體排斥效應，或離子間的排斥力可能造成現有研 究中可與SiO2@APTMS縮合量不足，因此可以改採以聚合反應將丙烯 酸先行與SiO2@APTMS縮合後，再依序接上其餘的丙烯酸單體成為 PAA，甚至從原有線性的PAA連接成為網狀的結構，提高PAA的修飾 量。
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