於第一篇專題演講中，Lucy Di Silvio 博士首先介紹近年來骨組織工程的發展，包括新材 料的開發、藥物釋放系統的引入、幹細胞的影響等，並簡介其在動物實驗與臨床方面的應用。
Sanjukta Deb 博士於第二篇專題演講中，專注於介紹用於骨組織工程的材料研發，同時也統
整了常用的物理與化學改質程序。由於此兩篇專題演講與本實驗室所進行的研究方向具有高 度相關性，因此讓吾人獲益良多。
會議的下午時間主要為論文發表，在本次的會議中，包含了 Oral、Student poster paper contest、Student oral paper contest 以及 Poster session 等，由於 Oral 有許多不同的主題，大會 總共安排了三間會議室作為演講廳同時進行，涵蓋的領域大致可分成 Implants、Biomaterials、
Computer-aided Dentistry與 Stem Cells。Student poster paper contest 與 Poster session 近 100 篇。口頭 論文發表之詳細題目可見附件一：
吾人於海報展示區和與會之專家學者進行討論
除了論文發表之外，本次大會亦安排有展示會及講習會等活動，即便是中午用餐時間，
我國參加此次會議的人員除吾人來自於工程領域外，尚有來自台大醫院與陽明醫院等醫 療體系的牙科與骨科醫生，這也與本會議促進工程與醫界人員交流的主旨不謀而合。
二、與會心得
在第一天的邀請演說中，Lucy Di Silvio 博士介紹了現今骨組織工程的發展趨勢，在材料 開發方面，與本實驗室目前的發展相符，即以人造生物材料與具特殊生物活性的因子相結合，
如此ㄧ來，將可使生物材料具有仿生(biomimetic)的性質，仿生材料的製備正是吾人目前研究 的重點之ㄧ。Lucy Di Silvio 博士介紹的另一重點為幹細胞的應用，幹細胞具有多元的分化性，
讓研究者有機會培養出完整的組織或器官，受到 Lucy Di Silvio 博士的啟發，吾人在回國後也 開始與台大醫院合作進行牙髓幹細胞的培養，希望能進一步將此一細胞與先前所開發的仿生 材料相結合，以期能有更多的發展性。
吾人此次的口頭報告主要是探討材料表面與細胞間的作用關係，我們在材料表面製造微 米至次微米的溝槽，並發現這些溝槽除了對細胞貼有助益外，還可以有效刺激骨組織的分化，
由於這純粹是以材料的物理性質控制細胞的行為，在現場得到不小的迴響。
三、 建議事項
綜觀本次的研討會與參訪之旅，讓吾人在知識與先端的研究課題上有了更一步的瞭解與 認知，並與亞洲數個生醫實驗室建立了雙方合作的開端，收效良多，證明國科會國合處訂定 之補助出席國際會議立意與成效俱佳，值得政府繼續鼓勵與資助。
四、 其他
本次會議後攜回會議論文集一本，亦帶回了部份牙科與骨科醫療商品的資訊，將提供給 國內研究者與相關廠商作為參考。
附件一 會議議程
12 November 2007
7.00-8.30 Registration
8.30-9.00
Opening Ceremony
( Ministry of Science and Technology)
The Role of National Science and Technology Development Agency for Oral Healthcare
(Assoc.Prof.Dr. Paritud Bhandhubanyong) 9.00-9.45
Current Advances in Tissue Engineering of Bone (Lucy Di Silvio, PhD )
9.45-10.00 Coffee Break
10.00-10.45 Current Advances in Biomaterial and Bone Substitute (Sanjukta Deb, PhD)
10.45-11.15
Dental Stem Cells: Potential Cellular Therapeutics for Dental Tissue Engineering and Regeneration
(Doosadee Hormdee, DDS, PhD)
11.15-12.00 Advances in Scaffolds for Bone Tissue Engineering (Sanjukta Deb, PhD)
12.00-13.00
Lunch Symposium (Zimmer)
Current Advances in Implant Design to Enhance Bone to Implant Contact and Long Term Bone Preservation
(Matthew Young, DDS)
Plenary Hall Room A Room B Room C
13.00-13.30
Cranial Bone Grafting for Reconstruction of
Maxillary and Mandibular
Defects (Prof. Peter D.
Waite)
Advances in Bioresorbable Scaffolds for Bone Tissue
Engineering ( Prof. Swee-Hin Teoh)
Applications of Rapid Prototyping
Technology in Dentistry and
Medicine (Jintamai Suwanprateeb,
PhD)
13.30-14.00
Bone Substitutes and Hyaluronic Acid: Outcome of
A Rabbit Research Study
and Clinical Experience with
Implants (Prof. Michael
Ong Ah Hup)
Advanced Bioreactors for Mechanical Loading and
Tissue Mechanical Property Measurement,
Tissue and Scaffolds (Prof. David Jones)
Bone Reconstruction in
Cranio Maxillofacial Surgery using RP
Technique: Past, Present and Future
(Jan Karel Th.
Haex, DDS)
14.00-14.30
The Use of a Mineralized Cancellous Bone Allograft (Puros) for Predictable Implant Dentistry
(Sirona)
Emdogain and Bone Ceramic (Assoc. Prof.
Anton Sculean)
Telenavigation in Implantology (Michael Truppe,
MD & Jan Karel
14.30-15.00 Poster Session Coffee Break
Plenary Hall
Room A Scaffold and Tissue
Engineering
Room B Computer-aided
Dentistry (1)
Room C Miscellaneous
15.00-15.15
Predictability of Pre-bent Advancement Plates for Use in Maxillomandibular
Advancement Surgery Prof.
Peter D Waite USA
Attachment, Proliferation and Behavior of Osteoblasts on Various
Chitosan-Collagen Scaffolds Dr. Wiroj Suphasiriroj Thailand
Adding FEA Simulation for the Fabrication of
Customized Titanium Tray
Implant for Mandible Reconstruction
Mr. Izhar Abd Aziz Malaysia
From the Lab to the Patients: the
Use of BMP for Validation of Guided versus Manual Implant Placement Prof.
Jef M. van der Zel Netherlands
The Effect of Various Size Coral Goniopora to Osteoblast Activity Dr.
Benny Sjariefsjah Latief Indonesia
Automatic Segmentation of
Dental CT using Entropic Threshold Mr.
Kitti Koonsanit Thailand
Bone Regeneration by œ-Tricalcium
Phosphate and Simvastatin Combination Dr.
Myat Nyan Japan
15.30-15.45
Minimal Invasive Sinus Lifting
Technique Assoc.Prof.Dr.
Hyung Jun Kim Korea
Osteoblastic Cell Behavior on Micro-Patterned Surfaces Asst.Prof.
Ming-Hua Ho Taiwan
DentiPlan: The Dental Implant
Planning Software Dr.
Saowapak Sotthivirat Thailand
Influence of Nicotine on the
Expression of Angiogenic Factors
in Distraction Osteogenesis Dr.
Li Ma Hong Kong
15.45-16.00
The Effects of the Graft's Periosteum on Healing Process
of Autogenous Iliac Bone Graft in Rat Calvarium Mr.
Cho Jinyong Korea
Influence of Continuous Traction on the Gene Expression of BMP-2 and
TGF-ß in Distraction Osteogenesis Dr. Li Wu
Zheng Hong Kong
The Application of Borland C++
BuilderTM and OpenGL®
Programming to Dentistry Software Development Mr.
Sasin Tiendee Thailand
Nanoencapsulated C-jun DNAzyme
Exhibits Anti-Osteosarcoma
Activity via Apoptosis Induction Dr.
Crispin Dass Australia
16.00-16.15
Bone Tissue Engineering Using Different Mesenchymal Stem Cells
Dr. Boworn Klongnoi Thailand
3D Geometric Transformation
for Dentistry Application Miss
Nonlapas Wongwaen
Thailand
Zoledronic Acid Inhibits Osteosarcoma at Multiple Levels Dr.
Crispin Dass Australia
17.30-20.00 Dinner Symposium
13 November 2007
7.00-8.30 Registration
8.30-9.00 Advances in Maxillofacial Polymeric Implant
(Sanjukta Deb, PhD)
9.30-9.45 Coffee Break 9.45-10.25
Application of Bioresorbable Plating System for Craniomaxillofacial Reconstructive Surgery
(Prof. Douglas P Sinn ) 10.25-10.55
The Skeletal Stability of Maxillary Osteotomies with
Poly-L-Lactic/Polyglycolic Acid Bone Fixation Devices in Orthognathic Surgery
(Prof. Peter D. Waite ) 10.55-11.35
Reconstruction of Oncologic Defect of Head and Neck Region With Autogenous Bone Graft
(Prof. Ghali E. Ghali)
11.35-12.00 The Role of Bangkok Biomaterial Center for Craniomaxillofacial Reconstruction
(Prof. Saranatra Waikakul )
12.00-13.00 Lunch Symposium
Plenary Hall Room A Room B
13.00-13.30
The Use of Demineralized Bone Matrix for Grafting During Lefort I And Chin
Osteotomies:
Techniques and Complications (Prof. Peter D.
Waite)
Introduction of Bone Formation With and
Without BMP (Mattabhorn Pornpatkul, MD)
Cellular Response to Materials and Surface (Lucy Di Silvio,
PhD)
13.30-14.00
Osseointegration of Newly Developed Thai
Dental Implant (Dr. Pathawee Khongkhunthian,
DDs, Dr.Med.Dent)
Biological Behavior of Zirconia Implants With
A
Zirconia-Hydroxyapatite Nanocomposite Coating
(Prof. Jef M. van der Zel)
Mathematical Modelling in Dental Implant Design and
Size Constant Balanced Refinements (Silvio
Emanuelli, DDS)
14.00-14.30
In Vitro Osteoproqenitor
Cellular Response to Surface Modified Titanium Implant
(Asst. Prof.
Lertrit Sarinnaphakorn)
Osteoinductive Implants - New Developments in Dental
Implantology (Asst. Prof. Yuelian M.
Liu)
Mathematical Modelling in Dental Implant Design and
Size Constant Balanced Refinements (Silvio
Emanuelli, DDS)
14.30-15.00 Poster Session
Coffee Break
Plenary Hall
Implants
Room A Biomaterials
Room B
Computer-aided Dentistry (2)
15.00-15.15
Osseointegration Time:
Peri-implant Bone Density
Analysis Asst.Prof.
Yosananda
A Comparison of Using Autogenous Bone Graft Combined With Bovine Hydroxy-Apatite and Autogenous Bone Graft Alone For Treatment of Alveolar Cleft Asst.Prof.
Enhancing the Oral and Maxillofacial Surgery Through Rapid Prototyping Process Mr.
Izhar Abd Aziz Malaysia
15.15-15.30
Dental Implant Complications and Associated
Risk Factors:
PSU Experiences Assoc.Prof.
Prisana Pripatnanont
Thailand
Bactericide Polymeric Based Cements for Dental Applications Mr.
Luis Rojo Spain
Mesh Warping Aided 3D Data Preparation for Dental Rapid
Prototyping. Dr. Chanjira Sinthanayothin Thailand
15.30-15.45
Immediate Placement of Coral Coated Dental Implant:
Bone Density Study Dr.
Sam'an Malik Masudi Malaysia
Calcium Phosphate Bone Cements Dr.
Sergey V Dorozhkin Russia
Neuron Network in Developing Software for Identifying Arch Form Dr.
Johan Arief Budiman Indonesia
15.45-16.00
Effects of Marginal Bone
Loss on Bone Strains Around Implants Placed
In Grafted Maxillary Sinuses:
3D-Finite Element Analysis
Mr. Samroeng Inglam Thailand
Influence of Preparation Methods on Properties
of Hydroxyapatite Ceramics Mr. Chokchai
Yatongchai Thailand
Development of Dental Software: Introducing ADTEC
DICOM Viewer. Mr. Krikamol Muandet Thailand
16.00-16.15
Influence of Adjacent Teeth
on Stress in Bones Surrounding Implants Mr.
Ekachai Chaichanasiri
Thailand
Green Chemical Synthesis of Silicon-Contained Calcium Phosphate Bioceramics Dr. Sergey
V Dorozhkin Russia
PACS (Picture Archiving Communication System) for
Dentistry Miss Nakintorn Patanachai Thailand
16.15-16.30 Closing ceremony
附件二 發表論文
Osteoblastic Cell Behavior on Micro-Patterned Surfaces Swary Fransiska1 and Ming-Hua Ho1,2
1 Department of Chemical Engineering, National Taiwan University of Science and Technology, Taipei, Taiwan
2 R&D Center of Membrane Technology and Department of Chemical Engineering, Chung Yuan University, Chungli, Taiwan
Cells would be inherently sensitive to their surroundings and some cells have been proved to respond to materials’ topography from micrometers to nanometers. In fact, the structures of natural extracellular matrix (ECM) is ordered in nano-scales. The adjustment of surface topography would be an approach to modulate cell’s behavior with the physical treatment only. Therefore, we try to culture rat osteosarcoma cells (ROS 1.7/28) on micro patterned biomaterials, and observe cell’s behavior.
Using photolithographic techniques, continuing micro grooved patterns were fabricated on silicon wafers. The wafers were patterned with varied groove depths of 0.8 to 1.1 µm, and varied groove widths of 1 to 15 µm. Firstly, the silicon wafer was cleaned ultrasonically in acetone solution, then continued by immersing wafers in piranha solution. After that, the wafers were copiously rinsed with DI water, and PBS, and sterilized under UV light. The osteoblastic cells, ROS cells, were seeded on the silicone wafer supplied with MEM, incubated at 37oC, and 5% CO2 and cultured for several periods. With fixation and dehydration, the cultured cells on silicone wafers can be observed with SEM and the other analysis.
From the SEM images, the cells were well proliferated after the culture for 1 week. At the same time, the “contact guidance” phenomena was found, where the cells elongated according to the surface pattern. On the contrary, there is no cell’s alignment on the smooth silicone surface. The tendency of cell’s elongation would be affected by aspect ratios of the grooves. This groove pattern could be utilized on biomedical implants to direct the growth of highly organized cells. Besides, the attached density of ROS cells would be different due to the differences in aspect ratios. The osteoblastic markers, such as calcium deposition, would be influenced, too.
附件三 邀請函