Outcome of intracytoplasmic injection of sperm obtained by testicular sperm extraction from 14 azoospermic men suffering from 47,XXY Non-mosaic Klinefelter's syndrome

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Outcome of intracytoplasmic injection of

sperm obtained by testicular sperm extraction

from 14 azoospermic men suffering from

47,XXY Non-mosaic Klinefelter's syndrome

李良明

Chun-Ming Chiang;Chuan-Ju Lin;Liang-Ming

Lee;Shih-Min chen;

摘要

Abstract

Objective: The purposes of this study were to evaluate the potential for testicular sperm extraction (TESE) from azoospermic patients with non-mosaic Klinefelter＇s syndrome and to determine the outcome of intracytoplasmic sperm injection (ICSI) using the

extracted testicular sperm sample. Materials and Methods: Fourteen couples suffering from primary infertility in which the male partner had the azoospermic non-mosaic 47,XXY karyotype (Klinefelter＇s syndrome) participated in this study. All of the women

underwent controlled ovarian hyperstimulation. Open testis biopsies were conducted 1 day prior to or on the day of oocyte retrieval. Motile sperm, extracted from the biopsied tissues in a wet preparation, were used for ICSI. The outcome of ICSI was evaluated from the fertilizatlon rate, embryo-cleavage rate, clinical pregnancy rate, and chromosomal status of resultant fetuses or delivered babies. Results: Sperm retrieval was successful I n eight of 14 patients (sperm retrieval rate, 57%). In total, 118 mature oocytes were injected with

extracted motile spermatozoa. This resulted in the production of70 fertilized oocytes (fertilization rate, 59%) and 67 embryos (cleavage rate, 96%). Among the eight women who underwent embryo transfer, six achieved clinical pregnancies (clinical pregnancy rate, 75%). The outcome of these pregnancies included one blighted ovum and the birth of four mal e and five female healthy babies. The live delivery rate was 62.5%. All of these babies were chromosomally and physiologically normal. Conclusion: This srudy demonstrates that azoospermic patients suffering from non-mosaic Klinefelter＇s syndrome can father their own genetic offspring when the TESE procedure is combined with ICSI and embryo transfer techniques. [Taiwanese j Obstet Gyneco/2004;43(2):88-96]