101學年度醫學檢驗生物技術學系碩士班考古題庫

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系所：醫學檢驗生物技術學系碩士班

高雄醫學大學 101 學年度研究所招生考試

科目：生物化學

1. Tools of Biochemistry (10%) 1)RT-PCR 2) site-directed mutagenesis 3) SDS-PAGE 4) Tandem MS 5) GFP

2. Diseases related Biochemistry (10%)

1) Maple syrup urine disease

2) African sleeping sickness

3) Obesity

4) Cystic fibrosis

5) Goat

3. Please draw a double-reciprocal or Lineweaver-Burk plots showing the effect of uncompetitive, competitive and noncompetitive inhibitors compare to uninhibitor on the kinetic of enzyme-catalyzed reactions. (10%)

4. Given two examples for each of Glycoprotein & Glycolipid with their biochemical and physiological functions. (10%)

5. In addition to the roles of information of nucleic acids, Nucleotides have a variety of three other functions of nucleotides in every cell. What are they? (10%)

6. Describe six types of DNA repair systems in E. coli. (10%)

7. Describe the roles of SnRNA, SnoRNA and miRNA. (10%)

8. Draw and describe and the path from insulin to GSK3 and glycogen synthase. (10%)

9. Define AKAP proteins and draw two types of AKAP: AKAP79 and AKAP250 in biosignaling. (10%)

10. Discuss the protein fate including their half-life and degradation. (10%)