THE EFFECTS OF ADDIN
ON THE BLOOD CLOTTIN
CALCIUM PHOSPHATE CE
Chia-Ching Lin
1
Department of Dental Hygiene, China Medical University, Taichung, Taiwan
2
Department of Fiber and Composite Materials, Feng Chia University, Taichung, Taiwan
3
Dental biology Laboratory, China Medical University, Taichung, Taiwan
INTRODUCTION
The purpose of this study was to investigate the effects of adding bismuth subgallate (BS) coagulant/antimicrobial agent)
time and cell viability.
METHODS
5, 10, 15wt% BS (5BS, 10BS, 15BS) were added to the CPC original powder (TTCP/DCPA) and mixed with
pastes were filled in a stainless mold to form specimens of Samples without BS were set as control group. The
5µL adult whole blood on each specimen formation which was tested every 15sec osteosarcoma cell viability were measured
analysis by ANOVA followed with Tukey’s test for post
Figure 1 Powders and liquid were mixed on a cold glass plate. The clotting time was determined after setting for 15 minutes using a blood taking needle every 15 sec.
THE EFFECTS OF ADDING COAGULANT/ANTIMICROBIAL AGENT
ON THE BLOOD CLOTTING TIME AND MG63 CELL ACTIVITY OF
CALCIUM PHOSPHATE CEMENT
Ching Lin1,3, Dan-Jae Lin1,3, Wen-Cheng Chen2
Department of Dental Hygiene, China Medical University, Taichung, Taiwan Department of Fiber and Composite Materials, Feng Chia University, Taichung, Taiwan
Dental biology Laboratory, China Medical University, Taichung, Taiwan E-mail: djlin@mail.cmu.edu.tw
was to investigate the effects of adding bismuth subgallate (BS) coagulant/antimicrobial agent) to the calcium phosphate cement (CPC) on its
BS (5BS, 10BS, 15BS) were added to the CPC original powder with 1M phosphoric acid at a liquid/ powder ratio of 0.35. in a stainless mold to form specimens of 3mm thickness and 6mm
ere set as control group. The clotting time was determined
on each specimen and measured the total time until protein fiber every 15sec by a blood taking needle. MG63 human
were measured by MTT assay at 1 hour and 24 analysis by ANOVA followed with Tukey’s test for post-hoc comparison.
Figure 1 Powders and liquid were mixed on a cold glass plate. The clotting time was 15 minutes using a blood taking needle every 15 sec.
OBIAL AGENT
ACTIVITY OF
Department of Dental Hygiene, China Medical University, Taichung, Taiwan Department of Fiber and Composite Materials, Feng Chia University, Taichung, Taiwan
Dental biology Laboratory, China Medical University, Taichung, Taiwan
was to investigate the effects of adding bismuth subgallate (BS) (a to the calcium phosphate cement (CPC) on its blood clotting
BS (5BS, 10BS, 15BS) were added to the CPC original powder
ratio of 0.35. The 3mm thickness and 6mm diameter.
determined by dropping the total time until protein fiber
human
hours. Data were
Figure 1 Powders and liquid were mixed on a cold glass plate. The clotting time was 15 minutes using a blood taking needle every 15 sec.
0 5 10 15 150 200 250 300 350 400 450 * b lo o d c lo ti n g t im e ( s e c ) BS contain in CPC (%) *
Figure 2 Clotting time of CPC 0%, 5%, 10%, and 15% BS. * means significant different to the control group (0% BS)
RESULTS AND DISCUSSION
The clotting time of 10BS (358±6sec) is significantly shorter than that of the control group (418±13sec, p<0.001). The clotting time of15BS is only 188±7sec. The one hour cell viability of each group containing BS was higher than that of the control group and blank group. After 24 hours, cell viabilities of BS-contained groups were still higher than that of the control group but there were no significant difference to the blank group.
CONCLUSION
Adding more than 10wt% BS can significantly accelerate the blood clotting on the CPC. Adding BS will increase the viability of MG63 cell at the first hour, but the cell viabilities at 24 hours were similar to the blank group.
REFERENCES
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2. H.H. Xu, S. Takagi, J.B. Quinn, L.C. Chow. J Biomed Mater Res A., 68, 725-734, 2004. 3. S.A. Puia, S.J. Renou, E.A. Rey, M.B. Guglielmotti, C.E. Bozzini. Int J Oral Maxillofac
