The development of Taiwan folk medicine hepatitis fourth report Xiao Jin Ying
Abstract
Xiao Jin Xiao Jin Ying Ying is Rosaceae fruits. The Liver Research Center in Taiwan folk medicine research, found little gold cherry obvious liver. In the anti- hepatitis B virus, the little gold cherry 50% Ethanolextract appear to inhibit HBV DNAP and DHBV DNAP's role, the other also found little gold cherry50% Ethanol extract also inhibited reverse the role of green paint. This thesis is based on the little gold cherry reverse anti-DHBV DNAP and HIV reverse painted green painted green activity for component separation, with the activity assay to track, in order to be able to issue a separate hepatoprotective activity, inhibition of DNA
polymerase, rdverse transcriptase of active ingredient, as basis for development of new drugs. The results are as follows: 1. By the inhibition of duck hepatitis B virus DNA polymerization activity of drawing the five components were separated to recover Ta Compound 1: to Oleanan-12-ene-3β ,24-diol, 2: 1 mixture
Compound 2: To Oleanolic acid, ursolic acid, 1: 1 mixture Compound 3: To Triterpenoid Saponin, the structure still be in prison Compound 4: To 2α, 3α, 19α- trihydroxyurs-12-en-28-oic acid (euscaphic ac-id) Compound 5: for the β-sitosterol (88.78%), Stigmasterol (11.22%) of the mixture in which Co-pound 3 as an active ingredient, IC50 llug / ml. 2. By the inhibition of M-MLV R-everse Transcriptase activity was isolated to recover Ta oleanolicacid, ursol-ic acid of the mixture (ibid.
compound 2) concentrations in 33.3ug.ml another74% of inhibition. 3.
Hepatoprotective effect was found by the methanol extracts of the CHC12 layer, n-HEXANE layer andH2Odialysis external solution (MW> 3500), oral or
intraperitoneal injection Jieyou liver. Components isolated from oleanolic acid and β-jsitost-erol composition of its hepatoprotective activity.
English Abstract
