Author(s): Lin, CW (Lin, Cheng-Wen); Wu, CF (Wu, Chia-Fang); Hsiao, NW (Hsiao, Nai- Wan); Chang, CY (Chang, Ching-Yao); Li, SW (Li, Shih-Wein); Wan, L (Wan, Lei); Lin, YJ (Lin, Ying-Ju); Lin, WY (Lin, Wei-Yong)
Title: Aloe-emodin is an interferon-inducing agent with antiviral activity against Japanese encephalitis virus and enterovirus 71
Source: INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS, 32 (4): 355-359 OCT 2008
Language: English Document Type: Article
Author Keywords: aloe-emodin; interferon signalling; Japanese encephalitis virus;
enterovirus 71
KeyWords Plus: NITRIC-OXIDE; IN-VITRO; INFECTION; ANTHRAQUINONES;
DERIVATIVES; INHIBITION; ACTIVATION; MICE
Abstract: In this study, aloe-emodin was identified as a potential interferon (IFN)-inducer by screening compounds from Chinese herbal medicine. Aloe-emodin showed low cytotoxicity to human HL-CZ promonocyte cells and TE-671 medulloblastoma cells and significantly
activated interferon-stimulated response element (ISRE) and gamma-activated sequence (GAS)-driven cis-reporting systems. Moreover, aloe-emodin upregulated expression of IFN- stimulated genes such as dsRNA-activated protein kinase and 2', 5'-oligoisoadenylate
synthase. Aloe-emodin resulted in significant activation of nitric oxide production. The antiviral activity of aloe-emodin against Japanese encephalitis virus (JEV) and enterovirus 71 (EV71) was evaluated using dose- and time-dependent plaque reduction assays in HL-CZ cells and TE-671 cells. The 50% inhibitory concentration (IC50) of aloe-emodin ranged from 0.50 mu g/mL to 1.51 mu g/mL for JEV and from 0.14 mu g/mL to 0.52 mu g/mL for EV71. Aloe-emodin showed clearly potent virus inhibitory abilities and achieved high therapeutic indices, in particular for HL-CZ cells. Therefore, the study demonstrated dose- and time-dependent actions of aloe-emodin on the inhibition of JEV and EV71 replication via IFN signalling responses. (C) 2008 Elsevier B. V. and the International Society of Chemotherapy. All rights reserved.
Addresses: [Lin, Cheng-Wen; Li, Shih-Wein] China Med Univ, Dept Med Lab Sci &
Biotechnol, Taichung 404, Taiwan; [Wu, Chia-Fang] China Med Univ, Dept Lab Med, Clin Virol Lab, Taichung 404, Taiwan; [Lin, Cheng-Wen; Wu, Chia-Fang; Chang, Ching-Yao] Asia Univ, Dept Biotechnol & Bioinformat, Taichung 413, Taiwan; [Wu, Chia-Fang] China Med Univ Hosp, Ctr Mol Med, Taichung 404, Taiwan; [Hsiao, Nai-Wan] Natl Changhua Univ Educ, Inst Biotechnol, Changhua 500, Taiwan; [Wan, Lei; Lin, Ying-Ju; Lin, Wei-Yong] China Med Univ, Dept Med Genet & Med Res, Taichung 404, Taiwan
Reprint Address: Lin, CW, China Med Univ, Dept Med Lab Sci & Biotechnol, 91 Hsueh Shih
Rd, Taichung 404, Taiwan.
E-mail Address: [email protected] Funding Acknowledgement:
Funding Agency Grant Number
National Science Council of R. O. C. (Taiwan) NSC95-2745-B-039-003-URD
China Medical University CMU95-054
National Science Council of R. O. C. (Taiwan) (NSC95-2745-B-039-003-URD); and China Medical University (CMU95-054).
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Publisher: ELSEVIER SCIENCE BV
Publisher Address: PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS ISSN: 0924-8579
DOI: 10.1016/j.ijantimicag.2008.04.018
29-char Source Abbrev.: INT J ANTIMICROBIAL AGENTS ISO Source Abbrev.: Int. J. Antimicrob. Agents
Source Item Page Count: 5
Subject Category: Infectious Diseases; Microbiology; Pharmacology & Pharmacy ISI Document Delivery No.: 345JJ
