Induction of differentiation in rat C6
glioma cells with saikosaponins
蔡妍菊
Tsai YJ;Chen IL;Horng LY;Wu RT
摘要
Abstract
The effects of saikosaponins (a, b1, b2, c, d), isolated from Bupleurum Radix, on the induction of differentiation in rat C6 glioma cells were studied. Saikosaponins a and d were shown to inhibit cell proliferation and alter cell morphology. In addition to cytostasis, the enzymatic activities of glutamine synthetase (GS) and 2,3-cyclic nucleotide
3-phosphohydrolase (CNP) were also noticeably increased after treatment with
saikosaponin a. Nevertheless, saikosaponin d only showed an increase of GS activity, no significant changes in CNP activity were found. These results suggest that saikosaponin a can induce the differentiation of C6 glioma cells into astrocytes and/or oligodendrocytes, but saikosaponin d can only induce the differentiation of C6 glioma cells into astrocytes. Copyright © 2002 John Wiley & Sons, Ltd.