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九十九學年度研究所碩士班考試入學

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九十九學年度研究所碩士班考試入學 環境工程學系碩士班乙組

環境微生物考科 第 1 頁,共 1 頁

- 1 - 1. (21%) Match the characteristics of eukaryotic cells in column A with their functions in

column B.

Column A Column B

Pericentriolar material 1 Digestive enzyme storage Chloroplasts 2 Oxidation of fatty acids Golgi complex 3 Microtubule formation

Lysosomes 4 Photosynthesis

Mitochondria 5 Protein synthesis

Peroxisomes 6 Respiration

Rough endoplasmic reticulum (ER) 7 Secretion

2. (14%) Which of the following can be used to sterilize a heat-labile solution stored in a plastic container? (請選出正確答案,並說明原因)

a. Gamma radiation b. Ethylene oxide c. Nonionizing radiation d. Autoclaving

e. Short-wavelength radiation

3. (35%) (a)Describe most probable number (MPN) method. (10%) (b) Describe polymerase chain reaction (PCR). (10%) (c) Describe MPN-PCR method and its application. (15%)

4. (30%) (a)測量微生物生長的方法有那些?(10%)(b)而在環工各方面如何應用?請就您所知 說明並解釋使用該方法之原因。(20%)

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