Author(s): Tsai, WL (Tsai, Wei Lung); Chen, HM (Chen, Hong-Ming); Hsieh, CH (Hsieh, Cheng-Hong); Lin, WF (Lin, Wen-Feng); Hwang, DF (Hwang, Deng-Fwu)
Title: A potential methodology for differentiation of ciguatgoxin-carrying species of moray eel Source: FOOD CONTROL, 20 (6): 575-579 JUN 2009
Language: English Document Type: Article
Author Keywords: Moray eel; Ciguatera; Food poisoning; PCR-RFLP; Cytochrome b gene KeyWords Plus: MITOCHONDRIAL-DNA SEQUENCES; CYTOCHROME-B GENE; PCR- RFLP ANALYSIS; PHYLOGENETIC-RELATIONSHIPS; CARIBBEAN CIGUATOXINS;
CICHLID FISHES; IDENTIFICATION; CIGUATERA; PRODUCTS; FRAGMENT
Abstract: A food poisoning incident due to ingestion of an unknown moray eel occurred in Taiwan. To identify the species of causative moray eel implicated in food poisoning. a 376-bp fragment sequence of cytochrome b gene was successfully obtained from four species of moray eel by using a pair of primers (L newest-1/H 15149). This fragment could be amplified using fish meat treated with different heating processes (100 degrees C for 30 min, 100 degrees C for 60 min, 121 degrees C for 15 min, and 121 degrees C for 30 min). After
sequencing, it was found that no variation in sequence was detected among individuals within each species. The species of causative moray eel implicated in food poisoning was judged as Gymnothorax javanicus based on sequence analysis. In addition, using restriction enzyme analysis, including Taq 1 and Sau96 1, could distinguish these four species of moray eel and identify the fish species of poisoning sample as G. javanicus. The toxicity of viscera in 24 specimens of four moray eel species was not detected, but the food poisoning sample was found to be toxic. Overall, this study proved that DNA. sequence and restriction enzyme analyses are useful in identifying that the causative moray eel species was G. javanicus. (C) 2008 Elsevier Ltd. All rights reserved.
Addresses: [Tsai, Wei Lung; Lin, Wen-Feng; Hwang, Deng-Fwu] Natl Taiwan Ocean Univ, Dept Food Sci, Chilung, Taiwan; [Chen, Hong-Ming] Natl Taiwan Ocean Univ, Dept
Aquaculture, Chilung, Taiwan; [Hsieh, Cheng-Hong] Asia Univ, Dept Appl Life Sci, Taichung, Taiwan
Reprint Address: Hwang, DF, Natl Taiwan Ocean Univ, Dept Food Sci, Chilung, Taiwan.
E-mail Address: dfhwang@mail.ntou.edu.tw Funding Acknowledgement:
Funding Agency Grant Number
National Science Council NSC-95-2313-B-019-024-MY3
Center for Marine Bioscience and Biotechnology, National 9552900 1A4
Taiwan Ocean University
Moray eels were identified by Dr. Kang-Tsao Shao, Institute of Cellular and Organismic Biology, Academia Sinica, Taipei, Taiwan, ROC and co-author Dr. Hong-Ming Chen by using morphological analysis. This study was partly supported by National Science Council (NSC- 95-2313-B-019-024-MY3) and Center for Marine Bioscience and Biotechnology, National Taiwan Ocean University (9552900 1A4).
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Cited Reference Count: 29
Times Cited: 0
Publisher: ELSEVIER SCI LTD
Publisher Address: THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND
ISSN: 0956-7135
DOI: 10.1016/j.foodcont.2008.08.011
29-char Source Abbrev.: FOOD CONTROL ISO Source Abbrev.: Food Control
Source Item Page Count: 5
Subject Category: Food Science & Technology ISI Document Delivery No.: 423BA
