樟芝降三酸甘油脂之功能研究

樟芝降三酸甘油脂之功能研究

 樟芝因具有其許多的經濟價值，而遭到濫採目前以經被政府列為保育類的植 物，為了要獲得更多的樟芝來源，科學家們即著手研發許多培養樟芝的方法

，包括了固態及液態發酵的培養方法。

過氧化體增殖活化的受體 (PPARs) 是一個轉錄因子，屬於 nuclear receptor sup erfamily ，當與其接合體 (ligand) 結合後，會進入細胞核，結合在具有 PPRE (peroxisome proliferators response element) 的基因啟動區。 PPAR ligands 已經 被用於在周邊組織增加胰島素的敏感度，而用來當作降低血糖的藥物。在 ins ulin resistant animal 的模式下和第二型 (non-insulin-dependent) 糖尿病的病患，

PPAR ligands 的使用，可降低血糖，胰島素和三酸甘油脂的濃度。在本研究 中，我們首先嘗試以 cell-base 的實驗方式，去研究樟芝是否具有降低血中脂 質的活性，結果顯示，野生樟芝子實體的水粗萃取物，可增加 PPAR?? transa ctivation activity 。基於此結果我們利用動物模式的實驗，來觀察野生樟芝子 實體及固培養樟芝，在餵食高膽固醇的 SD 老鼠的實驗下，兩者在降低血中 脂質 (hypolipidemic) 的效能。結果顯示野生樟芝子實體可以有效地減低血漿 的三酸甘油脂 (triglyceride) 和血糖的濃度，但是對於血漿的膽固醇並沒有影 響。另一方面，結果也顯示固態培養樟芝，對於血漿的膽固醇及三酸甘油脂 (triglyceride) 的濃度是不會有影響。由實驗結果，我們認為野生樟芝子實體可 能具有降三酸甘油脂的功能，並且優於固培養樟芝。

Study on the hypotriglyceridemic effect of Antrodia camphorata in rats with high-cholesterol diet



Antrodia camphorata (A. camphorata) is a kind of fungus and parasite on the inner heartwood

wall of Cinnamonum kanehirai in wilderness. It is a Taiwan-specific and known traditional Ch

inese medicine, and has been used to treatment of diarrhea, hypertension, itchy skin, and liver

cancer. It also exhibits several biological activities such as antioxidative and hepatoprotective

activities. To gain large amount of A. camphorata, several culture technologies have been dev

eloped, including solid-state culture and liquid-state fermantation. Peroxisome proliferators act

ivated receptors (PPARs) are transcription factors belonging to the nuclear receptor superfamil

y, and PPAR?? subtype has been described as a hypoglycemic agent that increase insulin sensi

tivity in peripheral tissues, and resulted in reducing blood glucose, insulin and triglyceride lev

els in insulin resistant animal and in type-2 (non-insulin-dependent) diabetic patients. In this st

udy, first we want to investigate the possibility of A. camphorata on the hypolipidemic activity

by cell-base experiment. Second, we want to compare the hypolipidemic effect between solid-

state culture and wild fruiting body of A. camphorate in animals. The results showed that wate

r extracts of wild fruiting bodies were able to increase the PPAR?? transactivation activity by t

ransfection of PPAR?? reporter plasmid. Base on the cell experiment, we have examined the h

ypolipidemic effect of solid-state culture and wild fruiting body of A. camphorata in SD rat fe

d with high cholesterol diet. The results showed that wild fruiting body was able to decrease th

e triglyceride and glucose levels, but could not affect the cholesterol level. On the other hand,

solid-state culture of A. camphorate had no significant effect on the blood levels of triglycerid

e and cholesterol. These results suggest that wild fruiting body may exhibit a hypotriglyceride

mic effect, and has more effect than solid-state culture of A . camphorate on the hypotriglyceri

demic activity.