國立成功大學「邁向頂尖大學計畫」
延攬優秀人才工作報告表
NCKU’s “Aim for the Top University Project”
Work Report Form for Distinguished Scholars
□續聘continuation of employment █離職resignation
100 年 7 月 13 日更新 受聘者姓名
Name of the Employee
鄭琮霖
█男 �女Male Female
聘 期 Period of Employment
from 102 年(y) 4 月(m) 1 日(d) to 102 年(y) 7 月(m) 31 日(d) 研究或教學或科技研發與
管理計畫名稱 The project title of research,
teaching, technology development and management
邁向頂尖大學計劃
計畫主持人
（申請單位主管）
Project Investigator (Head of Department/Center)
吳華林
補助延聘編號
Grant Number HUA - - -
一、 研究、教學、科技研發與管理工作全程經過概述。（由受聘人填寫）
Please summarize the entire research, teaching, or science and technology R&D and management work process (To be completed by the employee)
研究
High-glucose environment downregulate thrombomodulin expression that promotes diabetic wound healing via toll-like receptor 4 pathway
Diabetes causes the impairment of wound healing process which involves hemostasis, inflammation, and angiogenesis. Our previous studies demonstrated that membrane-bound thrombomodulin (TM) play a role on mediating keratinocyte differentiation, and the soluble TM (sTM) released by rhomboid serine protease has the effects on promoting cutaneous wound healing. However, the role of TM on diabetic skin remains unclear. In this study, we found the protein levels of TM and toll-like receptor 4 (TLR4) were significantly downregulated in high-glucose cultured human keratinocytes and diabetic patients’ primary keratinocytes.
Treatment of TNF-α antibody reversed the high-glucose caused TM downregulation;
furthermore treatment of TNF-α decreased the TM expression in a normal-glucose condition.
High-glucose environment also abolished the wound-triggered TM upregulation and sTM production in both HaCaT cells and streptozocin (STZ) diabetic mice. Notably, treatment of recombinant sTM could upregulate the TLR4 expression and promotes cutaneous wound healing in STZ mice, but these benefits were lost in Tlr4-deleted mice that with delayed healing rate in early inflammatory stage. Taken together, high-glucose environment may cause the inhibition of membrane-bound TM expression through the TNF-α pathway, and sTM should function as an inflammatory mediator on promoting diabetic wound healing through a TLR4-dependent pathway.
教學
生物化學(職治系必修四學分每周二小時、護理系必修三學分每周二小時)；生化所專題討 論必修一學分每周一小時；生物技術選修二學分
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二、研究或教學或科技研發與管理成效評估（由計畫主持人或單位主管填寫）
Please evaluate the performance of research, teaching or science and technology R&D and management Work: (To be completed by Project Investigator or Head of Department/Center)
(1)是否達到延攬預期目標？
Has the expected goal of recruitment been achieved?
是
(2)研究或教學或科技研發與管理的方法、專業知識及進度如何？
What are the methods, professional knowledge, and progress of the research, teaching, or R&D and management work?
符合進度
(3)受延攬人之研究或教學或科技研發與管理成果對該計畫(或貴單位)助益如何？
How have the research, teaching, or R&D and management results of the employed person given benefit to the project (or your unit)?
有助益
(4)受延攬人於補助期間對貴單位或國內相關學術科技領域助益如何？
How has the employed person, during his or her term of employment, benefited your unit or the relevant domestic academic field?
有助益
(5)具體工作績效或研究或教學或科技研發與管理成果：
Please describe the specific work performance, or the results of research, teaching, or R&D and management work:
Treatment of recombinant TM fragment reverses the downregulated TLR4 expression and decreased wound recovery that caused by high-glucose environment in vitro
Figure. Recombinant human TM fragment (hTMD23) promotes TLR4 expression and wound recovery in high-glucose cultured HaCaT. (a) Western blot analysis of TLR4 level in the high-glucose cultured HaCaT that treated with hTMD23 by indicated concentrations for 24 hours. (b) Wound recovery assay in high-glucose cultured HaCaT that were treated with various dose of hTMD23 for 72 hours. White dotted lines indicate the wound borders at beginning. (c) Quantitative representation of b. ** P < 0.01. Representative results from three independent experiments are shown.
(6)是否續聘受聘人? Will you continue hiring the employed person? □續聘Yes █不續聘No
※ 此報告表篇幅以三～四頁為原則。This report form should be limited to 3-4 pages in principle.
※ 此表格可上延攬優秀人才成果報告繳交說明網頁下載。
This report form can be downloaded in http://scholar.lib.ncku.edu.tw/explain/
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