Association among phthalate exposure, DNA methylation of RUNX3, and childhood allergy
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(2) Association among phthalate exposure, DNA methylation of RUNX3, and childhood allergy Wan-Ru Wang*, Department of Environmental and Occupational Health, College of Medicine, National Cheng Kung University, Tainan, Taiwan Nai-Yun Hsu, Department of Environmental and Occupational Health, College of Medicine, National Cheng Kung University, Tainan, Taiwan I-Ying Kuo, Department of Biochemistry and Molecular Biology, College of Medicine, National Cheng Kung University, Tainan, Taiwan Huey-Jen Su, Department of Environmental and Occupational Health, College of Medicine, National Cheng Kung University, Tainan, Taiwan. Background/Aim Environmental exposures could influence health status via the epigenetic mechanisms. Runt-related transcription factor 3 (RUNX3), a gene known to suppress allergic airway disease, was found to be excessively methylated in allergic subjects. Research has shown that exposure to phthalate esters, plasticizers widely used in plastic products, are related to the development of allergy morbidities. Yet, whether the effect of phthalate exposure on the imbalance of TH1 and TH2 pathway may associate with the regulation of DNA methylation is unclear. Thus, this study is aimed to verify the association among phthalate exposure, methylation of RUNX3, and the presence of childhood allergy and asthma. Methods A total of 28 pilot subjects (mean age 6.33 years) were randomly recruited from the previous population follow-up study in southern Taiwan. Each of them had detailed health examination, exposure concentrations of phthalate parental compounds in house settled dusts and metabolites in urine (µg/g creatinine). Their serum samples were used to analyse the total IgE level and DNA methylation status via Methylation Specific PCR (MSP) method. The methylation status is the ratio of “methylation level divided by un-methylation level”. Results Significant higher methylation status of the RUNX3 gene was shown in the group with urinary MBzP concentration greater than the median value (mean: 1.48 vs. 0.97, p = 0.004). For those total IgE higher than 100 kU/l, a bit higher of methylation status (1.23 vs.1.21) as well as the urinary MBzP concentration (26.37 vs. 18.97) were found, but without significant statistics might be due to the smaller sample size. Conclusions The preliminary results reveal that BBzP exposure may be associated with the changes of the DNA methylation status of RUNX3. The future work will be focused on the remaining 73 subjects, and to explore the underlying mechanism among exposure, DNA methylation and the outcome of interest..
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