植物基因轉殖 (Plant Gene Transformation)
教師：蘇睿智 辦公室：LS310 分機：2150
教室：ES 617
時間：Wed. 10:10 ~12:00 學分：2
計分：Midterm (50%)，Final (50%)
近些年來，因為分子生物學的進步，科學家們可以將控制各種性狀的基因自生物體內直接分離出來，
經過實驗室的操作將其連接到所謂的基因載體(vector)之後，再藉由基因轉殖(transformation)的方式，送 入我們所選定的植物體內。因為此一程序並不經過有性繁殖的授精過程，所以理論上我們可跨越種間不 和合的障礙，將無論是選殖(clone)自動物、植物、微生物、乃至病毒的有用基因送入植物體內表現，並進 而縮短整個育種流程的時間。本課程將依序介紹目前使用的植物基因轉殖方法及原理，以及其在基礎分 子生物學和作物育種上的應用。
9/19(三) Background Introduction--Plant breeding、overview of plant tissue culture 9/26 DNA、gene、chromosome、plasmid、and recombinant DNA technology
10/3 Infection mechanism of Agrobacterium tumefaciens and its Application in plant genetic transformation
10/10 National Holiday
10/17 PEG-mediated、electroporation、particle bombardment-mediated transformation 10/24 Selection、regeneration and confirmation of transgenic plants
10/31 Genetic analysis of transgenes inheritance in progeny generations 11/7 Application of transgenic plants in disease-resistant program 11/14 Midterm exam
11/21 Application of transgenic plants in stress-tolerance program 11/28 Application of transgenic plants in flower breeding program 12/5 Application of transgenic plants in industry
12/12 Application of transgenic plants in pharmaceutical production program
12/19 Application of transgenic in scientific research (T-DNA tagging, activation tagging…etc.) 12/26 Problem of gene silencing
1/2 Safety issue of GMO plant and foods 1/9 Patent application of transgenic crops 1/16 Final Exam
