則針對腸病毒 71 型(Entereovirus 71)作濃度的檢測作研究，已有初步的結果，如 下列所示：
Ab(Rabbit) w/BSA 0.05 濃度的樣品所影響，在電荷傳遞阻抗(Rct, 半圓直徑)有相當明顯的差異，也因此本實驗證明 Virus-BEA 對於不同濃度之 Ev71 病毒有不同的訊號反應。並將作為病毒濃度檢測器。
利用 sol-gel 設計之 ITO-BEA 應用於各類抗原之檢測
另一方面，本實驗室所設計之 Hp-BEA 在數據量測時間(一分鐘內)以及樣品反應時 間(六分鐘內)的研究上都有想當理想的結果，相較於傳統生化分析法也有長足的改 善。惟長時間製備過程與重複使用次數依然是本實驗室欲改進努力之目標。目前本 研究正在嘗試朝 sol-gel(polymer-film)設計為發展的目標，如同 POAP based Hp-BEA 一樣，其原理為利用生物相容性高的高分子單體，在電極表面產生長鏈網狀 聚集，形成孔洞性材質，同時將據有生化活性的抗體分子一定包覆進孔洞中，優點 為固定步驟快速，整理化學性質穩定，亦即縮短製備時間與增加 BEA 的重複使用性；
不同的是，本實驗目前欲利用導電玻璃(ITO，Indium Tin Oxide)來作為此類設計 的基材[130]，可省略掉工作電極研磨的實驗前置時間，又可以藉由其高透光度來 即時作光學的量測，如果該實驗設計成功，那將會是本系列實驗之中最省時，最低 成本，推廣價值最高的生物感測器設計，下圖為目前利用導電玻璃來生成 POAP 聚 合物的初步實驗，步驟同 POAP based Hp-BEA，在數次電聚合效應之後，可見表面 有一層咖啡色的聚合物薄膜生成，本實驗設計即是想利用該薄膜來固定偵測物分 子。
利用融溶態金球作 BEA 工作電極製備
本論文研究亦欲取代傳統商品化電極設計，朝客製化，微小化與低價化發展；研究 經驗指出，一般品質之較廉價金導線在電子顯微鏡下可見為數頗多的孔洞，推測其 原因應為拉線時的速度過快已致孔洞的產生，目前本實驗室正研究利用將金線重新 融溶為液體使其自然聚集為微小金球，再將金球置入於高分子塑膠中，接以導線即 完成一簡易之高品質工作電極，在電子顯微鏡下也證明孔洞數目明顯變少，推論可 圖 七.2 空白 ITO 玻璃 (左) 與 POAP 附著之 ITO 玻璃(右,紅色方框標示處)作比 較
增大電極之穩定程度與增加抗體固定數目已加大訊號反應。
作大量已知表現型之血液樣品測試以驗證 Hp-BEA 之準確度與穩定性
目前所有關於本論文研究的血液樣品皆來自於自願者提供，尚未有任何大規模的樣 品檢測，使得本研究尚停留在實驗階段而無法往臨床實驗上應用，而且 Hp 1-1 始 終有樣品取得不易的問題。目前本實驗室透過鄭財木博士的引薦，與台北榮民總醫 院潘如濱醫師作合作，短期之內應該可以有較為多數的血液樣品可作研究，如此即 有利於 Hp-BEA 的驗證與推廣。
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