未來展望

在之後實驗中，本研究會接著確認小型肝細胞之分離方式的確效性，除了利 用前文所採用經 Cytocentrifuge 後，利用免疫染色方式於螢光顯微鏡下所觀察所 得到的定性結外，亦希望利用 Flow Cytometer 將細胞懸浮液組成進一步的得到定 量的結果 (Adan et al., 2017)。可以將其結果與利用流式細胞儀 (Fluorescence Activated Cell Sorter，FACS) 分離之結果 (Ishii et al., 2017) 進行分離效果的比較。

也希望能分析培養天數更長 (> 14 天) 之細胞機能表現，並確認有分化為成熟肝 細胞潛能的小型肝細胞是否能維持其活性並超過前人所提及之 7 天體外生存情 況 (Choi and Diehl, 2009; Zeisberg et al., 2007) 。

另一方面，本研究希望能進一步的探討小型肝細胞長期培養於 PMMA 與 PDMS 微流道上之細胞形態及細胞活性、機能的差異性與 PDMS，藉由這些比較，

希望可更進一步模仿體內肝臟細胞於體外微流道之分佈情形：例如參照 Ljupcho Prodanov 利用雙層流道之結構進而模擬血管中之養份需經滲透才能到達肝實質細 胞的生理現象。另外一方面，相較前人研究在非實質細胞部份利用 Cell line 進行 共培養 (Prodanov et al., 2016) ，我們更希望能用初代細胞來進行共培養 (例如類 洞內皮細胞等)，以更模擬體內細胞的交互作用。最後，我們亦會進一步利用 NGS 及 qPCR 等方式，將在微流道上培養不同時間長之肝細胞與大鼠體內之肝細胞的 基因表現進行比較，試著找出與體內的肝細胞的基因表現最相似的培養時間，作 為最適化的培養時間，希望進一步的可以供後續的藥物測試使用。

doi:10.6342/NTU201803374 63

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