本研究說明丹皮酚前治療和血流阻斷後 30 min 治療能減少腦梗塞
面積和神經缺損，而血流阻斷後60 min 則沒有相似的效果，推論丹皮酚
對人類腦梗塞的治療有益，但療效與給予丹皮酚治療的time widow 有密
切關係。丹皮酚對於神經的保護作用可能與NF-κB 的途徑相關，至於
與AP-1 的關係需做進一步的研究，以釐清其彼此間的相互關係。
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英文摘要
Effect of paeonol at different time administration on cerebral infarct induced by ischemia-reperfusion injured Sprague-Dawley
rat
TSUNG-CHIEH LI
Major Professor:Ching-Liang Hsieh Institute of Chinese Medical Science
China Medical University
According to our previous study that the paeonol can decrease the brain infarction area and neurological deficit grading score. This time, the purpose of our study is to investigate the effect of paeonol at different time and the relation to nuclear factor-κB(NF-κB) and activator protein-1 (AP-1). The model of the ischemia-reperfusion injured cerebral infract was bilateral common carotid arteries and right middle cerebral artery occluded and reperfusion for 24 hours. The paeonol 20 mg/Kg was administered, respectively, from right femoral vein of rat before 20 min and 30、60 min after the cerebral blood was occluded. The therapeutic index of the paeonol is according to the cerebral infracted area and neurological deficit score. Our study also investigate the NF-κB and AP-1 activity between the infracted side and non-infarcted side. The results revealed that the paeonol 20 mg/Kg before 20 min and 30 min after the occluded from right femoral i.v injection can decrease the ischemia- reperfusion rats cerebral infracted area ad
neurological deficit score. But the paeonol 20 mg/Kg i.v injection after the occlusion 60 min doesn’t have the same effect. In addition, the NF-κB binding activity in the sham group (the cerebral artery didn’t occluded) have the highest level. The second is the paeonol treated group and followed the control group(the cerebral artery was occluded). Besides, the AP-1 binding activity were the same among the triple group.
In conclusion, paeonol pretreatment and 30 min after the occlusion can decrease cerebral infarcted area and neurological deficit score , but the
60 min after occluding the cerebral blood flow doesn’t have the same effect.
Therefore, we suggest that paeonol have the benefit of the human cerebral infarction. And the treatment effect is related with the time window of paeonol closely. Otherwise, paeonol have the neuron protection but the relation with the AP-1 should be for advanced study.
Keywords: Paeonol, Different time , Cerebral infarct, Neurological deficit, NF-κb, Activator protein-1