本研究自巨峰葡萄籽甲醇抽出物，以藥理試驗追蹤活性成分，所 萃取之乙酸乙酯層，單離得到三個化合物 catechin、gallic acid、epi-catechin。另外在正己烷層中，單離得到一個化合物 dilinoleyl stearyl glyceride。
在一連串針對 HL-60 血癌細胞，所做的藥理活性追蹤中顯示，從 甲醇粗抽物，一路選擇由乙酸乙酯抽出物繼續做分離的工作。乙酸乙 酯抽出物的藥理活性為 IC50 ＝ 23.58 μg/ml；EA2的藥理活性 IC50 ＝ 20.83 μg/ml；從 EA2劃分出的 Fr. A，其藥理活性為 IC50 ＝ 7.57 μg/
ml；最後所得的化合物 I，其藥理活性為 IC50 ＝ 3.1 μg/ml，經結構 鑑定為 gallic acid，由此可知所追蹤的成分應為 gallic acid 。
文獻考察(40) (41)中得知，從細胞型態上觀察，當 gallic acid 加入
HL-60 cell，48 小時後產生了凋亡小體，接著進行細胞週期試驗，細 胞停留在 G0-G1期，因此可了解 gallic acid 細胞增殖抑制的途徑，為 誘導細胞走向細胞凋亡的路徑。
另外，在針對 B16-F0、NCI-H226、J5、MDA KB-231cell 所做的 細胞抑制增殖實驗中得知，gallic acid 對 B16-F0、J5 cell 有抑制細胞 增殖的效果，之後可繼續深入了解其抑制細胞增殖的機轉。而 epi-catechin 與 epi-catechin 對所測試的四株細胞株沒有明顯的效果。
分離所得的化合物之美白試驗，gallic acid 在 1000 μM 下，五分鐘 內抑制酪胺酸酶的活性，抑制率約為 50%，較 epicatechin 與 catechin 佳，未來在美白產品上仍有發展的空間。
研究中並未分離出花青素類的化合物，筆者推測應是由於此類化 合物容易氧化分解成單體的 catechin、epi-catechin，而本研究之實驗 進行過程，採藥理活性成分追蹤的方式，因此所需的時間較長，導致 花青素類化合物的分解。
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