總結

為了能夠量測心肌細胞的膜外電位，本篇論文主要分為三大部分，分別是（1）微 電極陣列系統、（2）訊號的濾波放大過程與（3）資料擷取、處理與分析。

首先設計一組具有五種不同直徑大小的微電極陣列，並利用基本的黃光微影製程，

將此一微電極陣列製作於矽晶片上，接著以壓克力和 PCB 建構微電極陣列系統，並藉 由細胞培養過程，來確保其生物相容性。此外，利用電化學沉積方式在金電極上方成長 樹枝狀結構的金，以提升電極與細胞接觸之表面積；又在金電極上修飾 laminin 增加細 胞的貼附性。由於細胞電生理訊號十分微弱，因此本篇論文利用鎖相放大器作為訊號濾 波放大的處理。最後，以 LabVIEW 將資料擷取並記錄下來，進行快速傅立葉轉換對訊 號做頻譜分析；並藉由腎上腺素對心肌細胞之影響，來觀察電性量測的變化。

6.2 未來展望

在本篇論文中，已建立起一個可用於量測細胞電生理訊號的簡易平台，且已經能夠 利用所設計的微電極陣列系統，對分離出來的心肌細胞進行電生理量測。而根據章節 5.2.4 所討論的內容，我們假設所量測到的訊號是為心肌細胞的跨膜電位，且由於心肌細 胞之間 gap junction 的耦合(coupling)程度不同，以至於訊號傳遞會有延遲的現象；根據 模擬的結果，若當細胞與細胞之間的訊號延遲為 300 μs 的情況下，當細胞數目愈來愈 多，所得之波形與一般跨膜電位的波形也愈來愈不同；而細胞數目的多寡，可藉由改變 兩個相鄰電極的間距而改變，因此，未來可藉由嘗試縮短兩個相鄰電極之間的距離，使 得量測波形與一般跨膜電位的波形更為相近。由於細胞的電位變化往往可以反映出細胞 許多的生理現象，因此，在未來可實際利用此量測平台，做進一步的生醫量測。

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