Author(s): Lin, CW (Lin, Cheng-Wen); Huang, YW (Huang, Yi-Wei); Hu, RM (Hu, Rouh-Mei);
Chiang, KH (Chiang, Kai-Hung); Yang, TC (Yang, Tsuey-Ching)
Title: The role of AmpR in regulation of L1 and L2 beta-lactamases in Stenotrophomonas maltophilia
Source: RESEARCH IN MICROBIOLOGY, 160 (2): 152-158 MAR 2009 Language: English
Document Type: Article
Author Keywords: Stenotrophomonas maltophilia; beta-lactamase; AmpR KeyWords Plus: GRAM-NEGATIVE BACTERIA; ENTEROBACTER-CLOACAE;
PSEUDOMONAS-AERUGINOSA; KLEBSIELLA-PNEUMONIAE; INDUCIBLE CEPHALOSPORINASE; CITROBACTER-FREUNDII; MORGANELLA-MORGANII;
SALMONELLA-ENTERITIDIS; ESCHERICHIA-COLI; HAFNIA-ALVEI
Abstract: Stenotrophomonas maltophilia is known to produce at least two chromosomal- mediated inducible beta-lactamases, L1 and L2. Gene L2, which encodes a class A beta- lactamase, and the adjacent ampR gene form an ampR-class A beta-lactamase module. L1 belongs to the class B, beta-lactamase and has no neighbor ampR-like regulatory gene. In this study, the ampR-L2 module from S. maltophilia KH was compared with ampR-beta-lactamase modules from several microorganisms with respect to the AmpR and beta-lactamase proteins and the intergenic (IG) region. S. maltophilia and Xanthomonas campestris showed the most closely phylogenetic relationship among the microorganisms considered. The regulatory role of AmpR towards L1 and L2 was further analyzed. In the absence of an inducer, AmpR acted as an activator for L1 expression and as a repressor for L2 expression, whereas AmpR was an activator for both genes in an induced state. In addition, inducibility of L1 and L2 genes depended on the presence of AmpR. The ampR transcript was weakly and constitutively expressed, but was not autoregulated. (C) 2008 Elsevier Masson SAS. All fights reserved.
Addresses: [Lin, Cheng-Wen; Huang, Yi-Wei; Chiang, Kai-Hung; Yang, Tsuey-Ching] China Med Univ, Dept Med Lab Sci & Biotechnol, Taichung 40402, Taiwan; [Hu, Rouh-Mei] Asia Univ, Dept Biotechnol & Bioinformat, Taichung 413, Taiwan
Reprint Address: Yang, TC, China Med Univ, Dept Med Lab Sci & Biotechnol, 91 Hsueh Shih Rd, Taichung 40402, Taiwan.
E-mail Address: [email protected] Funding Acknowledgement:
Funding Agency Grant Number
China Medical University CMU-95-155 CMU-95-102
National Science Council NSC 97-2320-B-039-028
This research was supported by grant CMU-95-155 and CMU-95-102 from China Medical University and grant NSC 97-2320-B-039-028 from the National Science Council.
Cited References: ALONSO A, 1997, ANTIMICROB AGENTS CH, V41, P1140.
AVISON MB, 2001, ANTIMICROB AGENTS CH, V45, P413.
BARNAUD G, 1998, ANTIMICROB AGENTS CH, V42, P2352.
BAUERNFEIND A, 1996, ANTIMICROB AGENTS CH, V40, P221.
BONNET R, 2002, ANTIMICROB AGENTS CH, V46, P3316, DOI 10.1128/AAC.46.10.3316- 3319.2002.
BRADFORD PA, 1997, ANTIMICROB AGENTS CH, V41, P563.
CAMPBELL JIA, 1989, BIOCHEM J, V260, P803.
DATZ M, 1994, EUR J BIOCHEM, V226, P149.
FORTINEAU N, 2001, J ANTIMICROB CHEMOTH, V47, P207.
GAILLOT O, 1997, J ANTIMICROB CHEMOTH, V39, P85.
GIRLICH D, 2000, ANTIMICROB AGENTS CH, V44, P1470.
HANSON ND, 1999, CURR PHARM DESIGN, V5, P881.
HONORE N, 1986, EMBO J, V5, P3709.
HU RM, 2008, ANTIMICROB AGENTS CH, V52, P1198, DOI 10.1128/AAC.00682-07.
JACOBS C, 1997, CELL, V88, P823.
KARKHOFFSCHWEIZ.RR, 1994, GENE, V140, P7.
KONG KF, 2005, ANTIMICROB AGENTS CH, V49, P4567, DOI 10.1128/AAC.49.11.4567- 4575.2005.
LINDBERG F, 1985, P NATL ACAD SCI USA, V82, P4620.
LINDBERG F, 1987, J BACTERIOL, V169, P758.
LINDQUIST S, 1989, J BACTERIOL, V171, P3746.
LODGE JM, 1990, BIOCHEM J, V272, P627.
MAHLEN SD, 2003, J ANTIMICROB CHEMOTH, V51, P791, DOI 10.1093/jac/dkg133.
NAAS T, 1994, P NATL ACAD SCI USA, V91, P7693.
NAAS T, 1995, ANTIMICROB AGENTS CH, V39, P629.
NADJAR D, 2000, FEMS MICROBIOL LETT, V187, P35.
NADJAR D, 2001, ANTIMICROB AGENTS CH, V45, P2324.
NAKANO R, 2004, ANTIMICROB AGENTS CH, V48, P1151, DOI 10.1128/AAC.48.4.1151- 1158.2004.
NORDMANN P, 1993, ANTIMICROB AGENTS CH, V37, P939.
NORMARK S, 1995, MICROB DRUG RESIST, V1, P111.
OKAZAKI A, 2008, ANTIMICROB AGENTS CH, V52, P1525, DOI 10.1128/AAC.01485-07.
PAPANICOLAOU GA, 1990, ANTIMICROB AGENTS CH, V34, P2200.
PHILIPPON A, 2002, ANTIMICROB AGENTS CH, V46, P1.
POIREL L, 1999, ANTIMICROB AGENTS CH, V43, P769.
RASMUSSEN BA, 1996, ANTIMICROB AGENTS CH, V40, P2080.
REISBIG MD, 2002, J ANTIMICROB CHEMOTH, V49, P557.
SAINO Y, 1982, ANTIMICROB AGENTS CH, V22, P564.
SAINO Y, 1984, ANTIMICROB AGENTS CH, V25, P362.
SCHELL MA, 1993, ANNU REV MICROBIOL, V47, P597.
SCHWEIZER HP, 1995, GENE, V158, P15.
SEOANE A, 1992, ANTIMICROB AGENTS CH, V36, P1049.
TREPANIER S, 1997, ANTIMICROB AGENTS CH, V41, P2399.
VERDET C, 2006, ANTIMICROB AGENTS CH, V50, P607, DOI 10.1128/AAC.50.2.607- 617.2006.
WENG SF, 2004, ANTIMICROB AGENTS CH, V48, P209, DOI 10.1128/AAC.48.1.209- 215.2004.
WIEDEMANN B, 1998, DRUG RESIST UPDATE, V1, P223.
Cited Reference Count: 44 Times Cited: 1
Publisher: ELSEVIER SCIENCE BV
Publisher Address: PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS ISSN: 0923-2508
DOI: 10.1016/j.resmic.2008.11.001
29-char Source Abbrev.: RES MICROBIOL ISO Source Abbrev.: Res. Microbiol.
Source Item Page Count: 7 Subject Category: Microbiology ISI Document Delivery No.: 425BE
