Author(s): Lai, IH (Lai, I-Hsiang); Tsao, JH (Tsao, Jung Hsu); Lu, YP (Lu, Yi Ping); Lee, JW (Lee, Jai Wei); Zhao, X (Zhao, Xin); Chien, FL (Chien, Feng Lin); Mao, SJT (Mao, Simon J. T.) Title: Neutrophils as one of the major haptoglobin sources in mastitis affected milk
Source: VETERINARY RESEARCH, 40 (3): Art. No. 17 MAY-JUN 2009 Language: English
Document Type: Article
Author Keywords: haptoglobin; neutrophil; mastitis; MAC-T cells; immunocytochemistry KeyWords Plus: BOVINE MAMMARY-GLAND; ACUTE-PHASE PROTEINS; SUBCLINICAL MASTITIS; BETA-LACTOGLOBULIN; CLINICAL MASTITIS; ESCHERICHIA-COLI;
MESSENGER-RNA; CELL-DAMAGE; DAIRY-COWS; ANTIOXIDANT
Abstract: The antioxidant haptoglobin (Hp) is an acute-phase protein responsive to infectious and inflammatory diseases. Hp and somatic cell counts (SCC) are sharply elevated in bovine milk following intramammary administration of endotoxin or bacteria. However, the sources of milk Hp responsible for such increases are not fully understood. The purpose of this study was to de. ne the source of milk Hp from dairy cows with naturally occurring mastitis. Quarter milk samples selected from 50 dairy cows were separated into four groups according to SCC as group A: < 100 (n = 19); B: 100-200 (n = 10); C: 201-500 (n = 10); and D: > 500 x 10(3) (n = 11) cells/mL. Our results reveal that milk Hp concentrations were correlated with SCC (r = 0.742; P < 0.01), and concentrations in group D were similar to 10-fold higher than in group A.
Reverse transcriptase-polymerase chain reaction (RT-PCR) analysis indicates that the milk somatic cells from group D were not only capable of synthesizing Hp but could also markedly increase Hp mRNA expression. Western blot, immunocytochemistry, double confocal immuno fluorescence, and Hp releasing experiments demonstrate that neutrophils were associated with the biosynthesis and release of Hp in milk. It further shows that Hp was significantly elevated in the epithelium of mammary gland tissue with mastitis and was also expressed in the cultured mammary epithelial cells. We propose that neutrophils and epithelial cells may play an essential role in elevating milk Hp in addition to previous suggestions that Hp may be derived from mammary tissues and circulation.
Addresses: [Lai, I-Hsiang; Chien, Feng Lin; Mao, Simon J. T.] Natl Chiao Tung Univ, Inst Biochem Engn, Coll Biol Sci & Technol, Hsinchu, Taiwan; [Tsao, Jung Hsu] Yong Rong Dairy Farm, Chyayi, Taiwan; [Lu, Yi Ping] Pingtung Cty Livestock Dis Control Ctr, Pingtung, Taiwan;
[Lee, Jai Wei] Natl Pingtung Univ Sci & Technol, Dept Trop Agr & Int Cooperat, Pingtung, Taiwan; [Zhao, Xin] McGill Univ, Dept Anim Sci, Quebec City, PQ, Canada; [Mao, Simon J. T.]
Asia Univ, Dept Biotechnol, Taichung, Taiwan
Reprint Address: Mao, SJT, Natl Chiao Tung Univ, Inst Biochem Engn, Coll Biol Sci &
Technol, 75 Po Ai St, Hsinchu, Taiwan.
E-mail Address: [email protected]
Funding Acknowledgement:
Funding Agency Grant Number
National Science Council, Taiwan
NSC (94-95)-2313-B-009-001 95-2313-B009-003-MY2 97-2313-B-009-001-MY2 NSC 94-2313-B-020-023
This work was supported by Grants NSC (94-95)-2313-B-009-001, 95-2313-B009-003-MY2 and 97-2313-B-009-001-MY2 (SJTM) and NSC 94-2313-B-020-023 (JWL) from the National Science Council, Taiwan, R.O.C. We also thank James Lee of the National Chiao Tung University for critical review of this manuscript.
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Publisher: EDP SCIENCES S A
Publisher Address: 17, AVE DU HOGGAR, PA COURTABOEUF, BP 112, F-91944 LES ULIS CEDEX A, FRANCE
ISSN: 0928-4249 Article Number: 17
DOI: 10.1051/vetres:2008055 29-char Source Abbrev.: VET RES ISO Source Abbrev.: Vet. Res.
Source Item Page Count: 12
Subject Category: Veterinary Sciences ISI Document Delivery No.: 428VF
