Author(s): Huang, CY (Huang, Chih-Yang); Yang, LC (Yang, Li-Chiu); Liu, KY (Liu, Kuan-Yu);
Liao, PH (Liao, Pao-Hsin); Chou, JIY (Chou, Janet Ing-Yuh); Chou, MY (Chou, Ming-Yung);
Lin, WW (Lin, Wei-Wen); Yang, JJ (Yang, Jaw-Ji)
Title: RhoGDI beta-induced hypertrophic growth in H9c2 cells is negatively regulated by ZAK Source: JOURNAL OF BIOMEDICAL SCIENCE, 16: Art. No. 11 JAN 22 2009
Language: English Document Type: Article
KeyWords Plus: GDP-DISSOCIATION INHIBITOR; CARDIOMYOBLAST CELLS; LY-GDI;
GTPASE; EXPRESSION; PROTEIN; PHOSPHORYLATION; METASTASIS; BINDING;
KINASE
Abstract: We found that overexpression of RhoGDI beta, a Rho GDP dissociation inhibitor, induced hypertrophic growth and suppressed cell cycle progression in a cultured
cardiomyoblast cell line. Knockdown of RhoGDI beta expression by RNA interference blocked hypertrophic growth. We further demonstrated that RhoGDI beta physically interacts with ZAK and is phosphorylated by ZAK in vitro, and this phosphorylation negatively regulates RhoGDI beta functions. Moreover, the ZAK-RhoGDI beta interaction may maintain ZAK in an inactive hypophosphorylated form. These two proteins could negatively regulate one another such that ZAK suppresses RhoGDI beta functions through phosphorylation and RhoGDI beta
counteracts the effects of ZAK by physical interaction. Knockdown of ZAK expression in ZAK- and RhoGDI beta-expressing cells by ZAK-specific RNA interference restored the full
functions of RhoGDI beta.
Addresses: [Yang, Li-Chiu; Liu, Kuan-Yu; Liao, Pao-Hsin; Chou, Ming-Yung; Yang, Jaw-Ji]
Chung Shan Med Univ Hosp, Sch Dent, Taichung 402, Taiwan; [Huang, Chih-Yang] China Med Univ, Grad Inst Chinese Med Sci, Taichung 404, Taiwan; [Huang, Chih-Yang] China Med Univ, Grad Inst Basic Med Sci, Taichung 404, Taiwan; [Huang, Chih-Yang] Asia Univ, Dept Hlth & Nutr Biotechnol, Taichung 413, Taiwan; [Yang, Li-Chiu] Chung Shan Med Univ Hosp, Inst Med, Taichung 402, Taiwan; [Chou, Janet Ing-Yuh] Midwestern Univ Chicago, Coll Osteopath Med, Chicago, IL USA; [Lin, Wei-Wen] Vet Gen Hosp, Ctr Cardiovasc, Taichung 407, Taiwan
Reprint Address: Yang, JJ, Chung Shan Med Univ Hosp, Sch Dent, Taichung 402, Taiwan.
E-mail Address: [email protected]; [email protected];
[email protected]; [email protected]; [email protected]; [email protected];
[email protected]; [email protected] Funding Acknowledgement:
Funding Agency Grant Number
National Science Council (NSC) NSC96-2311-B-040-003-
This work was supported by grants to J. J. Y. from the National Science Council (NSC) (NSC96-2311-B-040-003-), Taiwan.
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Publisher: BIOMED CENTRAL LTD
Publisher Address: CURRENT SCIENCE GROUP, MIDDLESEX HOUSE, 34-42 CLEVELAND ST, LONDON W1T 4LB, ENGLAND
ISSN: 1021-7770 Article Number: 11
DOI: 10.1186/1423-0127-16-11
29-char Source Abbrev.: J BIOMED SCI ISO Source Abbrev.: J. Biomed. Sci.
Source Item Page Count: 9
Subject Category: Medicine, Research & Experimental ISI Document Delivery No.: 419DD
