圖一；以pH 3-10 的 IPG strip 與 12％的 SDS page 的二維電泳，分離感染石 斑魚虹彩病毒後的金目鱸肌肉細胞蛋白質之銀染色圖。
1
表一；利用mascot 軟體進行蛋白質身分比對的結果
（A） （B）
圖三；（A）以 1.0％洋菜膠體電泳分析 Initiation factor 4A 1B （eIF4A1B）
PCR 增幅結果；圖三（B）為金目鱸 eIF4A1B 核苷酸定序與斑馬魚 eIF4A1B 核苷酸序列的比對結果。
（C）
三；（C）為 eIF4A1B 金目鱸胺基酸序列與斑馬魚胺基酸序列的比對結果。
圖
（A） （B）
（C）
圖四；（A）以 1.0％洋菜膠體電泳分析 ADP-ribosylation factor 1（Arf-1） PCR 增幅結果；（B）金目鱸 Arf-1 核苷酸定序與斑馬魚核苷酸序列的比對 結果；（C）Arf-1 金目鱸胺基酸序列與斑馬魚胺基酸序列的比對結果。
（A） （B）
圖五；（A）以1.0％洋菜膠體電泳分析Ribose 5 phosphate isomerase（RPIA）
PCR增幅結果；（B）為RPIA核苷酸定序與斑馬魚核苷 酸序列的比 對結果。
（A） （B）
圖五；（C）為 Ribose 5 phosphate isomerase（RPIA）金目鱸胺基酸序列與斑 馬魚胺基酸序列的比對結果。
（A） （B）
與斑馬魚核苷酸序列的比對結果。
圖六；（A）以 1.0％洋菜膠體電泳分析 Tyrosine Hydroxylase（TH） PCR 增 幅結果，（B）為 TH 核苷酸定序
（C）
圖六；（C）為 TH 金目鱸胺基酸序列與斑馬魚胺基酸序列的比對結果。
（A） （B）
（C）
七；（A）以 1.0％洋菜膠體電泳分析 Phosphoglycerate mutase 1（PGAM1）
的 比對結果；（C）為 PGAM1 金目鱸胺基酸序列與斑馬魚胺基酸序列 的比對結果。
增幅結果；圖七（B）為 PGAM1 核苷酸定序與斑馬魚核苷酸序列 圖
圖八；
0 1 2 3 4 5 6 7 8 9 10 11
c 30 45 60 90 120 150 180 Post infection
Fold
12
使用realtime-PCR 監控不同感染時間點 eIF4A1B）的基因表現量。
圖九；使用 監控不同感染時間點 因表現量。
0 1 2 3 4 5 6 7 8 9 10 11 12
c 30 45 60 90 120 150 180 Post infection
Fold
realtime-PCR Arf-1 的基
十；使用realtime-PCR 監控不同感染時間點 RPIA 的基因表現量。
0 1 2 3 4 5 6 7 8 9 10 11 12
c 30 45 60 90 120 150 180 Post infection
Fold
圖
圖十一；使用 監控不同感染時間點 的基因表現量。
01 23 45 6 78 109 1112
c 30 45 60 90 120 150 180 Post infection
Fold
realtime-PCR TH
se 1
（PGAM1）的表現量。
0 12 3 4 56 7 8 109 11 12
c 30 45 60 90 120 150 180 Post infection
Fold
圖十二；使用realtime-PCR 監控不同感染時間點 Phosphoglycerate muta
圖十三；以1.0 PCR 增幅的結果。
圖十四；pCMV-tag4 的載體
進行序列分析的結果。
圖十五；是將全長的Arf-1，
圖十六；金目鱸ADP-ribosylation factor 1 like 的種源分析圖。
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