在自然環境下，厭氣性真菌的主要碳源來自於構造及組成相當複雜的木質纖維 基質，因此可推測當厭氣性真菌利用這些物質時，其所生成的cellulosome組成應是非 常多樣性的。另外，厭氣性真菌cellulosome的研究相對於厭氣性細菌而言仍處於初期 的探索階段，對於此兩系統的差異比較，如scaffoldin的相關研究各組成份生成調控機 制仍是相當缺乏的。本研究已從結晶性纖維誘導培養條件下成功純化N. patriciarum J11 cellulosome，且利用LC/MS/MS鑑定參與分解結晶性纖維的cellulosme組成分。在 此條件下，N. patriciarum J11 cellulosome組成份至少已包含了內切型(endo- type)、非 還原端外切型(exo-type, non-reducing end)及還原端外切型(exo-type, reducing end)纖維 素分解酶。由於目前基因資料庫中，厭氣性真菌相關的分解酶相對於厭氣性細菌及好 氣性微生物顯得不足，因此若只以蛋白質鑑定為工具，則將造成許多目標蛋白質無法 被鑑定。相關領域研究中，其基礎的基因庫建立、相關基因釣取以及厭氣性真菌基因 組解碼仍是必要且關鍵的環節。本研究中對於厭氣性真菌dockerin與scaffoldin的結合 關係已有了初步的探討，未來，隨著基因資料庫中厭氣性真菌相關的分解酶序列的增 加，我們仍可藉由蛋白質工程技術進一步研究並控制dockerin與scaffoldin或厭氣性真 菌cohesin間的結合作用以提高其未來應用之潛力。近年隨著研究工具日新月異及相關 技術的精進，未來在厭氣性真菌cellulosome的基礎研究上，除了scaffoldin基因及相關 基 因 的釣 取與 研 究外， 另 可以 即時 聚 合酶鏈 式 反應 (real time PCR) 、轉 錄 體 學 (transcriptome)方法探究厭氣性真菌cellulosome各組成分在核酸及蛋白質層次上的調 控機制，進而了解在自然環境中厭氣性真菌為了生存其所演化、發展出的高效率分解 機制。這些相關的研究基可提供研究人員在第二代生質酒精生產或其它應用上的基 礎。
參考文獻
1. 陳又嘉。1997。絕對厭氣性真菌的分離培養與纖維分解能力之探討。國立台灣
大學農業化學研究所碩士論文。
2. 黃雅惠。2005。厭氣性真菌 Neocallimastix frontalis 木聚醣酶基因之選殖、表現 與重組蛋白酵素特性分析。國立台灣大學微生物與生化學研究所博士論文。 characterization of type II dockerin module from the cellulosome of Clostridium thermocellum: calcium-induced effects on conformation and target recognition.
Biochemistry 44:2173-2182.
7. Ali, B. R., L. Zhou, F. M. Graves, R. B. Freedman, G. W. Black, H. J. Gilbert, and G.
P. Hazelwood. 1995. Cellulases and hemicellulases of the anaerobic fungus
Piromyces constitute a multiprotein cellulose-binding complex and are encoded by multigene families. FEMS Microbiol Lett 125:15-21.
8. Angenent, L. T., K. Karim, M. H. Al-Dahhan, B. A. Wrenn, and R.
Domiguez-Espinosa. 2004. Production of bioenergy and biochemicals from industrial and agricultural wastewater. Trends Biotechnol 22:477-485.
9. Anthony, P. J. T., D. R. Davies,K. Guii,M. I. Lawrence,B. B. Nielsen,A. Rickers,and M. K. Theodorou. 1994. Anaerobic fungi in herbivorous animals. Mycol Res
98:129-152.
10. Aylward, J. H., K. S. Gobius,G. P. Xue,G. D. Simpson,and B. P. Dalrymple. 1999.
The Neocallimastix patriciarum cellulase,CelD,contains three almost identical catalytic domains with high specific activities on Avicel. Enzyme Microb Tech 24:609-614.
11. Bansal, P., M. Hall, M. J. Realff, J. H. Lee, and A. S. Bommarius. 2009. Modeling cellulase kinetics on lignocellulosic substrates. Biotechnol Adv 27:833-848.
12. Barichievich, E. M., and R. E. Calza. 1990. Supernatant protein and cellulase activities of the anaerobic ruminal fungus Neocallimastix frontalis EB188. Appl Environ Microbiol 56:43-48.
13. Bauchop, T., and D. O. Mountfort. 1981. Cellulose fermentation by a rumen
anaerobic fungus in both the absence and the presence of rumen methanogens. Appl Environ Microbiol 42:1103-1110.
14. Bayer, E. A., J. P. Belaich, Y. Shoham, and R. Lamed. 2004. The cellulosomes:
multienzyme machines for degradation of plant cell wall polysaccharides. Annu Rev Microbiol 58:521-554.
15. Bayer, E. A., H. Chanzy, R. Lamed, and Y. Shoham. 1998. Cellulose, cellulases and cellulosomes. Curr Opin Struct Biol 8:548-557.
16. Bayer, E. A., R. Kenig, and R. Lamed. 1983. Adherence of Clostridium
thermocellum to cellulose. J Bacteriol 156:818-827.
17. Bayer, E. A., and R. Lamed. 1986. Ultrastructure of the cell surface cellulosome of Clostridium thermocellum and its interaction with cellulose. J Bacteriol
167:828-836.
18. Bayer, E. A., E. Setter, and R. Lamed. 1985. Organization and distribution of the cellulosome in Clostridium thermocellum. J Bacteriol 163:552-559.
19. Beg, Q. K., M. Kapoor, L. Mahajan, and G. S. Hoondal. . 2001. Microbial xylanases and their industrial applications: a review. Appl Microbiol Biotechnol 56:326-338.
20. Beldman, G., M. F. Searle-Van Leeuwen, F. M. Rombouts, and F. G. Voragen. 1985.
The cellulase of Trichoderma viride. Purification, characterization and comparison of all detectable endoglucanases, exoglucanases and beta-glucosidases. Eur J Biochem 146:301-308.
21. Belghith, H., S. Ellouz-Chaabouni, and A. Gargouri. 2001. Biostoning of denims by Penicillium occitanis (Pol6) cellulases. J Biotechnol 89:257-262.
22. Bhat, M. K., and S. Bhat. 1997. Cellulose degrading enzymes and their potential industrial applications. Biotechnol Adv 15:583-620.
23. Boraston, A. B., D. N. Bolam, H. J. Gilbert, and G. J. Davies. 2004.
Carbohydrate-binding modules: fine-tuning polysaccharide recognition. Biochem J 382:769-781.
24. Bothast, R. J., and M. A. Schlicher. 2005. Biotechnological processes for conversion of corn into ethanol. Appl Microbiol Biotechnol 67:19-25.
25. Bowman, B. H., J. W. Taylor, A. G. Brownlee, J. Lee, S. D. Lu, and T. J. White.
1992. Molecular evolution of the fungi: relationship of the Basidiomycetes, Ascomycetes, and Chytridiomycetes. Mol Biol Evol 9:285-296.
26. Brookman, J. L., G. Mennim, A. P. Trinci, M. K. Theodorou, and D. S. Tuckwell.
2000. Identification and characterization of anaerobic gut fungi using molecular methodologies based on ribosomal ITS1 and 185 rRNA. Microbiology 146 ( Pt 2):393-403.
27. Brownlee, A. G. 1988. A rapid DNA isolation procedure applicable to many refractory filamentous fungi. Fungal Genet Newslett 35:8-9.
28. Brownlee, A. G. 1989. Remarkably AT-rich genomic DNA from the anaerobic fungus Neocallimastix. Nucleic Acids Res. 17:1327-1335.
29. Carvalho, A. L., F. M. Dias, J. A. Prates, T. Nagy, H. J. Gilbert, G. J. Davies, L. M.
Ferreira, M. J. Romao, and C. M. Fontes. 2003. Cellulosome assembly revealed by the crystal structure of the cohesin-dockerin complex. Proc Natl Acad Sci U S A 100:13809-13814.
30. Chen, H., X. L. Li, D. L. Blum, E. A. Ximenes, and L. G. Ljungdahl. 2003. CelF of Orpinomyces PC-2 has an intron and encodes a cellulase (CelF) containing a carbohydrate-binding module. Appl Biochem Biotechnol 105 -108:775-785.
31. Chen, H., X. L. Li, and L. G. Ljungdahl. 1997. Sequencing of a
1,3-1,4-beta-D-glucanase (lichenase) from the anaerobic fungus Orpinomyces strain PC-2: properties of the enzyme expressed in Escherichia coli and evidence that the gene has a bacterial origin. J Bacteriol 179:6028-6034.
32. Chen, Y. C., S. D. Tsai, H. L. Cheng, C. Y. Chien, C. Y. Hu, and T. Y. Cheng. 2007.
Caecomyces sympodialis sp. nov., a new rumen fungus isolated from Bos indicus.
Mycologia 99:125-130.
33. Chikamatsu, G., K. Shirai, M. Kato, T. Kobayashi, and N. Tsukagoshi. 1999.
Structure and expression properties of the endo-beta-1,4-glucanase A gene from the filamentous fungus Aspergillus nidulans. FEMS Microbiol Lett 175:239-245.
34. Choi, S. K., and L. G. Ljungdahl. 1996. Structural role of calcium for the
organization of the cellulosome of Clostridium thermocellum. Biochemistry 35:4906-4910.
35. Chu, C. Y., C. W. Tseng, P. Y. Yueh, C. H. Duan, and J. R. Liu. 2011. Molecular cloning and characterization of a beta-glucanase from Piromyces rhizinflatus. J Biosci Bioeng 111:541-546.
36. Collins, T., C. Gerday, and G. Feller. 2005. Xylanases, xylanase families and extremophilic xylanases. FEMS Microbiol Rev 29:3-23.
37. Coughlan, M. P. 1988. Comparative viochemistry of fungal and bacterial cellulolytic enzyme system. Academic Press, London.
38. Csiszar, E., A. Losonczi, G. Szakacs, I. Rusznak, L. Bezur, and J. Reicher. 2001.
Enzymes and chelating agent in cotton pretreatment. J Biotechnol 89:271-279.
39. Delmer, D. P., and Y. Amor. 1995. Cellulose biosynthesis. Plant Cell 7:987-1000.
40. Denman, S., G. P. Xue, and B. Patel. 1996. Characterization of a Neocallimastix patriciarum cellulase cDNA (celA) homologous to Trichoderma reesei
cellobiohydrolase II. Appl Environ Microbiol 62:1889-1896.
41. Dijkerman, R., J. Ledeboer, H. J. M. Op den Camp, R. A. Prins, and C. van der Drift.
1997. The Anaerobic Fungus Neocallimastix sp. Strain L2: Growth and production of (hemi)cellulolytic enzymes on a range of carbohydrate substrates. Curr Microbiol 34:91-96.
42. Dijkerman, R., H. J. Op den Camp, C. Van der Drift, and G. D. Vogels. 1997. The role of the cellulolytic high molecular mass (HMM) complex of the anaerobic fungus Piromyces sp. strain E2 in the hydrolysis of microcrystalline cellulose. Arch Microbiol 167:137-142.
43. Ding, S. Y., E. A. Bayer, D. Steiner, Y. Shoham, and R. Lamed. 2000. A scaffoldin of the Bacteroides cellulosolvens cellulosome that contains 11 type II cohesins. J Bacteriol 182:4915-4925.
44. Eberhardt, R. Y., H. J. Gilbert, and G. P. Hazlewood. 2000. Primary sequence and enzymic properties of two modular endoglucanases, Cel5A and Cel45A, from the anaerobic fungus Piromyces equi. Microbiology 146 ( Pt 8):1999-2008.
45. Endo, K., Y. Hakamada, S. Takizawa, H. Kubota, N. Sumitomo, T. Kobayashi, and S.
Ito. 2001. A novel alkaline endoglucanase from an alkaliphilic Bacillus isolate:
enzymatic properties, and nucleotide and deduced amino acid sequences. Appl Microbiol Biotechnol 57:109-116.
46. Fanutti, C., T. Ponyi, G. W. Black, G. P. Hazlewood, and H. J. Gilbert. 1995. The conserved noncatalytic 40-residue sequence in cellulases and hemicellulases from anaerobic fungi functions as a protein docking domain. J Biol Chem
270:29314-29322.
47. Fedorak, P. M., and S. E. Hrudey. 1983. A simple apparatus for measuring gas production by methanogenic cultures in serum bottles. Environ Technol Lett.
4:425-432.
48. Fierobe, H. P., E. A. Bayer, C. Tardif, M. Czjzek, A. Mechaly, A. Belaich, R. Lamed, Y. Shoham, and J. P. Belaich. 2002. Degradation of cellulose substrates by
cellulosome chimeras. Substrate targeting versus proximity of enzyme components.
J Biol Chem 277:49621-49630.
49. Fierobe, H. P., A. Mechaly, C. Tardif, A. Belaich, R. Lamed, Y. Shoham, J. P.
Belaich, and E. A. Bayer. 2001. Design and production of active cellulosome chimeras. Selective incorporation of dockerin-containing enzymes into defined functional complexes. J Biol Chem 276:21257-21261.
50. Fierobe, H. P., S. Pages, A. Belaich, S. Champ, D. Lexa, and J. P. Belaich. 1999.
Cellulosome from Clostridium cellulolyticum: molecular study of the
Dockerin/Cohesin interaction. Biochemistry 38:12822-12832.
51. Fleury, D., P. Domaingue, C. Gillard, R. Touitou, and P. Mollat. 2007. Expression, purification, characterization and crystallization of a recombinant human cytosolic beta-glucosidase produced in insect cells. Protein Expr Purif 52:96-103.
52. Fujino, Y., K. Ogata, T. Nagamine, and K. Ushida. 1998. Cloning, sequencing, and expression of an endoglucanase gene from the rumen anaerobic fungus
Neocallimastix frontalis MCH3. Biosci Biotechnol Biochem 62:1795-1798.
53. Garcia-Vallve, S., A. Romeu, and J. Palau. 2000. Horizontal gene transfer of glycosyl hydrolases of the rumen fungi. Mol Biol Evol 17:352-361.
54. Gibbs, M. D., R. A. Reeves, G. K. Farrington, P. Anderson, D. P. Williams, and P. L.
Bergquist. 2000. Multidomain and multifunctional glycosyl hydrolases from the extreme thermophile Caldicellulosiruptor isolate Tok7B.1. Curr Microbiol 40:333-340.
55. Glikes, N. R., B. Henrissat,D. G. Kilburn,R. C. Miller,and R. A. J. Warren. 1991.
Domains in microbial beta-1,4-glycanase:sequence convervation function and enzyme families. Microbiol Rev 55:303-315.
56. Gray, K. A., L. Zhao, and M. Emptage. 2006. Bioethanol. Curr Opin Chem Biol 10:141-146.
57. Haltrich, D., B. Nidetzky, D. K. Kulbe, W. Steiner, and S. Zupancic. 1996.
Production of fungal xylanases. Bioresour Technol 58.
58. Handelsman, T., Y. Barak, D. Nakar, A. Mechaly, R. Lamed, Y. Shoham, and E. A.
Bayer. 2004. Cohesin-dockerin interaction in cellulosome assembly: a single Asp-to-Asn mutation disrupts high-affinity cohesin-dockerin binding. FEBS Lett 572:195-200.
59. Harchand, R. K., and S. Singh. 1997. Extracellular cellulase system of a
thermotolerant streptomycete: Streptomyces albaduncus. Acta Microbiol Immunol Hung 44:229-239.
60. Harhangi, H. R., A. Akhmanova, P. J. Steenbakkers, M. S. Jetten, C. van der Drift, and H. J. Op den Camp. 2003. Genomic DNA analysis of genes encoding
(hemi-)cellulolytic enzymes of the anaerobic fungus Piromyces sp. E2. Gene 314:73-80.
61. Hayashi, H., M. Takehara, T. Hattori, T. Kimura, S. Karita, K. Sakka, and K.
Ohmiya. 1999. Nucleotide sequences of two contiguous and highly homologous xylanase genes xynA and xynB and characterization of XynA from Clostridium thermocellum. Appl Microbiol Biotechnol 51:348-357.
62. Henrissat, B., M. Claeyssens, P. Tomme, L. Lemesle, and J. P. Mornon. 1989.
Cellulase families revealed by hydrophobic cluster analysis. Gene 81:83-95.
63. Henrissat, B., Y. Popineau, and J. C. Kader. 1988. Hydrophobic-cluster analysis of plant protein sequences. A domain homology between storage and lipid-transfer proteins. Biochem J 255:901-905.
64. Ho, Y. W., and D. J. Barr. 1995. Classification of anaerobic gut fungi from
herbivores with emphasis on rumen fungi from Malaysia. Mycologia 87:655-677.
65. Hobson, P. N., and R. J. Wallace. 1982. Microbial ecology and activities in the rumen: part 1. Crit Rev Microbiol 9:165-225.
66. Horikoshi, K. 1999. Alkaliphiles: some applications of their products for biotechnology. Microbiol Mol Biol Rev 63:735-750.
67. Ilmen, M., A. Saloheimo, M. L. Onnela, and M. E. Penttila. 1997. Regulation of cellulase gene expression in the filamentous fungus Trichoderma reesei. Appl Environ Microbiol 63:1298-1306.
68. Imai, T., C. Boisset, M. Samejima, K. Igarashi, and J. Sugiyama. 1998.
Unidirectional processive action of cellobiohydrolase Cel7A on Valonia cellulose microcrystals. FEBS Lett 432:113-116.
69. Jindou, S., A. Soda, S. Karita, T. Kajino, P. Beguin, J. H. Wu, M. Inagaki, T. Kimura, K. Sakka, and K. Ohmiya. 2004. Cohesin-dockerin interactions within and between Clostridium josui and Clostridium thermocellum: binding selectivity between cognate dockerin and cohesin domains and species specificity. J Biol Chem 279:9867-9874.
70. Kennedy, J. F. 1988. Carbohydrate Chemistry. Oxford University.
71. Klyosov, A. A. 1990. Trends in biochemistry and enzymology of cellulose degradation. Biochemistry 29:10577-10585.
72. Kosugi, A., K. Murashima, Y. Tamaru, and R. H. Doi. 2002. Cell-surface-anchoring role of N-terminal surface layer homology domains of Clostridium cellulovorans EngE. J Bacteriol 184:884-888.
73. Kunkel, T. A. 1985. Rapid and efficient site-specific mutagenesis without phenotype selection. Proc Natl Acad Sci U S A 82:488-492.
74. Lamed, R., and E. A. Bayer. 1988. The cellulosome of Clostridium thermocellum.
Adv Appl Microbiol 33:1-46.
75. Lamed, R., E. Setter, and E. A. Bayer. 1983. The cellulosome - a discrete cell surface organelle of Clostridium thermocellum which exhibits separate antigenic, cellulose-binding and various cellulolytic activities. Biotechnol Bioeng Symp 13:163-181.
76. Leibovitz, E., and P. Beguin. 1996. A new type of cohesin domain that specifically binds the dockerin domain of the Clostridium thermocellum cellulosome-integrating protein CipA. J Bacteriol 178:3077-3084.
77. Lemaire, M., H. Ohayon, P. Gounon, T. Fujino, and P. B´eguin. 1995. OlpB, a newouter layer protein of Clostridium thermocellum, and binding of its S-layer-like domains to components of the cell envelope. J Bacteriol 177:2451-2459.
78. Leschine, S. B. 1995. Cellulose degradation in anaerobic environments. Annu Rev Microbiol 49:399-426.
79. Li, J., B. Heath,and L. Packer. 1992. The phylogenetic relationships of anaerobic chytridiomycetous gut fungi (Neocallimastigaceae) and the Chytridiomycota.I.
Cladistic analysis of rRNA sequences. Canad J Bot 70:1738-1740.
80. Li, J., B. Heath,and L. Packer. 1992. The phylogenetic relationships of anaerobic chytridiomycetous gut fungi (Neocallimastigaceae) and the Chytridiomycota.I.
Cladistic analysis of rRNA sequences. Canad J Bot 70:1738-1740.
81. Lowe, S. E., M. K. Theodorou, A. P. J. Trinci, and R. B. Hespell. 1985. Growth of anaerobic rumen fungi on defined and semi-defined media lacking rumen fluid. J Gene Microbiol 131:2225-2229.
82. Lowe, S. E., M. K. Theodorou, and A. P. Trinci. 1987. Cellulases and xylanase of an anaerobic rumen fungus grown on wheat straw, wheat straw holocellulose, cellulose, and xylan. Appl Environ Microbiol 53:1216-1223.
83. Lowe, S. E., M. K. Theodorou, and A. P. Trinci. 1987. Growth and fermentation of an anaerobic rumen fungus on various carbon sources and effect of temperature on development. Appl Environ Microbiol 53:1210-1215.
84. Lytle, B., C. Myers, K. Kruus, and J. H. Wu. 1996. Interactions of the CelS binding ligand with various receptor domains of the Clostridium thermocellum cellulosomal scaffolding protein, CipA. J Bacteriol 178:1200-1203.
85. Lytle, B. L., B. F. Volkman, W. M. Westler, M. P. Heckman, and J. H. Wu. 2001.
Solution structure of a type I dockerin domain, a novel prokaryotic, extracellular calcium-binding domain. J Mol Biol 307:745-753.
86. Lytle, B. L., B. F. Volkman, W. M. Westler, and J. H. Wu. 2000. Secondary structure and calcium-induced folding of the Clostridium thermocellum dockerin domain determined by NMR spectroscopy. Arch Biochem Biophys 379:237-244.
87. Margit, S. a. U. B. S. 2000. S-Layer Proteins. J. Bacteriol. 182:859-868.
88. Mechaly, A., H. P. Fierobe, A. Belaich, J. P. Belaich, R. Lamed, Y. Shoham, and E.
A. Bayer. 2001. Cohesin-dockerin interaction in cellulosome assembly: a single hydroxyl group of a dockerin domain distinguishes between nonrecognition and high affinity recognition. J Biol Chem 276:9883-9888.
89. Mechaly, A., S. Yaron, R. Lamed, H. P. Fierobe, A. Belaich, J. P. Belaich, Y. Shoham, and E. A. Bayer. 2000. Cohesin-dockerin recognition in cellulosome assembly:
experiment versus hypothesis. Proteins 39:170-177.
90. Mernitz, G., A. Koch, B. Henrissat, and G. Schulz. 1996. Endoglucanase II (EGII) of Penicillium janthinellum: cDNA sequence, heterologous expression and promotor analysis. Curr Genet 29:490-495.
91. Miras, I., F. Schaeffer, P. Beguin, and P. M. Alzari. 2002. Mapping by site-directed mutagenesis of the region responsible for cohesin-dockerin interaction on the surface of the seventh cohesin domain of Clostridium thermocellum CipA.
Biochemistry 41:2115-2119.
92. Mountfort, D. O., and C. G. Opin. 1994. Anaerobic fungi. Marcel Dekker, Inc., New York.
93. Mountfort, D. O., and R. A. Asher. 1985. Production and regulation of cellulase by two strains of the rumen anaerobic fungus Neocallimastix frontalis. Appl Environ Microbiol 49:1314-1322.
94. Mountfort, D. O., and R. A. Asher. 1983. Role of catabolite regulatory mechanisms in control of carbohydrate utilization by the rumen anaerobic fungus Neocallimastix frontalis. Appl Environ Microbiol 46:1331-1338.
95. Murashima, K., C. L. Chen, A. Kosugi, Y. Tamaru, R. H. Doi, and S. L. Wong. 2002.
Heterologous production of Clostridium cellulovorans engB, using protease-deficient Bacillus subtilis, and preparation of active recombinant cellulosomes. J Bacteriol 184:76-81.
96. Nielsen, B. B., W. Y. Zhu, M. S. Dhanoa, A. P. J.Trinci, and M. K.Theodorou. 2002.
Comparison of the growth kinetics of anaerobic gut fungi onwheat strawin batch culture. Aanerobe 8:216-222.
97. Noach, I., F. Frolow, H. Jakoby, S. Rosenheck, L. W. Shimon, R. Lamed, and E. A.
Bayer. 2005. Crystal structure of a type-II cohesin module from the Bacteroides cellulosolvens cellulosome reveals novel and distinctive secondary structural elements. J Mol Biol 348:1-12.
98. Ohara, H., J. Noguchi, S. Karita, T. Kimura, K. Sakka, and K. Ohmiya. 2000.
Sequence of egV and properties of EgV, a Ruminococcus albus endoglucanase containing a dockerin domain. Biosci Biotechnol Biochem 64:80-88.
99. Orpin, C. G. 1975. Studies on the rumen flagellate Neocallimastix frontalis. J Gen Microbiol 91:249-262.
100. Ozcan, N., C. Cunningham, and W. J. Harris. 1996. Cloning of a cellulase gene from the rumen anaerobe Fibrobacter succinogenes SD35 and partial characterization of the gene product. Lett Appl Microbiol 22:85-89.
101. Pages, S., A. Belaich, J. P. Belaich, E. Morag, R. Lamed, Y. Shoham, and E. A.
Bayer. 1997. Species-specificity of the cohesin-dockerin interaction between Clostridium thermocellum and Clostridium cellulolyticum: prediction of specificity determinants of the dockerin domain. Proteins 29:517-527.
102. Pages, S., A. Belaich, C. Tardif, C. Reverbel-Leroy, C. Gaudin, and J. P. Belaich.
1996. Interaction between the endoglucanase CelA and the scaffolding protein CipC of the Clostridium cellulolyticum cellulosome. J Bacteriol 178:2279-2286.
103. Pason, P., A. Kosugi, R. Waeonukul, C. Tachaapaikoon, K. Ratanakhanokchai, T.
Arai, Y. Murata, J. Nakajima, and Y. Mori. 2010. Purification and characterization of a multienzyme complex produced by Paenibacillus curdlanolyticus B-6. Appl Microbiol Biotechnol 85:573-580.
104. Pearce, P. D., and T. Bauchop. 1985. Glycosidases of the rumen anaerobic fungus Neocallimastix frontalis grown on cellulosic substrates. Appl Environ Microbiol 49:1265-1269.
105. Percival Zhang, Y. H., M. E. Himmel, and J. R. Mielenz. 2006. Outlook for cellulase improvement: screening and selection strategies. Biotechnol Adv 24:452-481.
106. Perret, S., A. Belaich, H. P. Fierobe, J. P. Belaich, and C. Tardif. 2004. Towards designer cellulosomes in Clostridia: mannanase enrichment of the cellulosomes produced by Clostridium cellulolyticum. J Bacteriol 186:6544-6552.
107. Perret, S., L. Casalot, H. P. Fierobe, C. Tardif, F. Sabathe, J. P. Belaich, and A.
Belaich. 2004. Production of heterologous and chimeric scaffoldins by Clostridium acetobutylicum ATCC 824. J Bacteriol 186:253-257.
108. Pessoa, A. J., I. C. Roberto, M. Menossi, R. R. Dos Santos, S. O. Fillho, and T. C. V.
Penna. 2005. Perspectives on bioenergy and biotechnology in Brazil. Appl. Biochem.
Biotechnol. 121-124:59-70.
109. Phillips, M. W., and G. L. Gordon. 1989. Growth characteristics on cellobiose of three different anaerobic fungi isolated from the ovine rumen. Appl Environ Microbiol 55:1695-1702.
110. Qiu, X., B. Selinger, L. Yanke, and K. Cheng. 2000. Isolation and analysis of two cellulase cDNAs from Orpinomyces joyonii. Gene 245:119-126.
111. Raghothama, S., R. Y. Eberhardt, P. Simpson, D. Wigelsworth, P. White, G. P.
Hazlewood, T. Nagy, H. J. Gilbert, and M. P. Williamson. 2001. Characterization of a cellulosome dockerin domain from the anaerobic fungus Piromyces equi. Nat Struct Biol 8:775-778.
112. Sakamoto, S., G. Tamura, K. Ito, T. Ishikawa, K. Iwano, and N. Nishiya. 1995.
Cloning and sequencing of cellulase cDNA from Aspergillus kawachii and its expression in Saccharomyces cerevisiae. Curr Genet 27:435-439.
113. Salamitou, S., K. Tokatlidis, P. Beguin, and J. p. Aubert. 1992. Involvement of separate domains of the cellulosomal protein S1 of Clostridium thermocellum in binding to cellulose and in anchoring of catalytic subunits to the cellulosome. FEBS Lett 304:89-92.
114. Sambrook, J., D. W. Russell. 2001. Molecular Cloning:a laboratory manual, third ed.
Cold Spring Harbor, New York.
115. Schmoll, M., and C. P. Kubicek. 2003. Regulation of Trichoderma cellulase formation: lessons in molecular biology from an industrial fungus. A review. Acta Microbiol Immunol Hung 50:125-145.
116. Schwarz, W. H. 2001. The cellulosome and cellulose degradation by anaerobic bacteria. Appl Microbiol Biotechnol 56:634-649.
117. Shimon, L. J., E. A. Bayer, E. Morag, R. Lamed, S. Yaron, Y. Shoham, and F.
Frolow. 1997. A cohesin domain from Clostridium thermocellum: the crystal structure provides new insights into cellulosome assembly. Structure 5:381-390.
118. Shoseyov, O., M. Takagi, M. A. Goldstein, and R. H. Doi. 1992. Primary sequence analysis of Clostridium cellulovorans cellulose binding protein. Proc Natl Acad Sci U S A 89:3483-3487.
119. Singh, A., and K. Hayashi. 1995. Microbial cellulases: protein architecture,
molecular properties, and biosynthesis. Adv Appl Microbiol 40:1-44.
120. Spinelli, S., H. P. Fierobe, A. Belaich, J. P. Belaich, B. Henrissat, and C. Cambillau.
2000. Crystal structure of a cohesin module from Clostridium cellulolyticum:
implications for dockerin recognition. J Mol Biol 304:189-200.
121. Steenbakkers, P. J., A. Freelove, B. Van Cranenbroek, B. M. Sweegers, H. R.
Harhangi, G. D. Vogels, G. P. Hazlewood, H. J. Gilbert, and H. J. Op den Camp.
2002. The major component of the cellulosomes of anaerobic fungi from the genus Piromyces is a family 48 glycoside hydrolase. DNA Seq 13:313-320.
122. Steenbakkers, P. J., X. L. Li, E. A. Ximenes, J. G. Arts, H. Chen, L. G. Ljungdahl,
122. Steenbakkers, P. J., X. L. Li, E. A. Ximenes, J. G. Arts, H. Chen, L. G. Ljungdahl,