交聯性質(如 fibrinogen 與 thrombin)[8,75]的水膠材料，可當作未來使用 的生物材料，應可有效降低實驗過程中對細胞的傷害，也期望可以用這
些前人證實過可以使血管生成的生物材料使得建構出的肝臟組織中也有
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附錄一 實驗儀器
(Olympus, IX71, IX73)，連接彩色攝影機後搭配相位差濾鏡便可拍攝較為立體之 生物樣本照片；連接汞燈並搭配螢光濾鏡後便可分別拍攝不同顏色之螢光照片，最後再利用電腦軟體疊圖。
即時細胞培養觀察系統與細胞培養箱
本實驗使用之細胞培養箱包含裝設有即時攝影系統的培養箱(Cultured cell monitored system, ASTEC)以及一般培養箱(MCO-19AIC, Panasonic)，用來維持體 外細胞培養所需之環境以及拍攝細胞生長狀態的照片。
無菌操作臺
本實驗使用無菌操作臺(4BH-24, HIGH TEN Scientific Corp.)是為了建立一無 菌環境，並在此環境下操作細胞相關實驗。
DAQ 介面卡
為了使用Labview (National Instrument)電腦軟體來控制電極的電壓施加與否，
使用DAQ 介面卡(USB-6509, National Instrument)連至電路板來進行控制。
繼電器電路板
電訊號經電壓放大器放大後傳入繼電器電路板，本實驗使用之電路板含有 48 個繼電器(LU-5, RAYEX)以控制各個腳位的開啟或關閉，而繼電器的開關則由 電腦軟體Labview (National Instrument)連接 DAQ 介面卡與電路板來控制。此電 路板之輸出電壓會聯接一具有48 個腳位的排線，最後接至加裝帶狀電纜連接器 (CC-125FRRC, Yokowo)以利輸入訊號至電控微流體平臺上。
點光源曝光機
本研究使用此UV 曝光設備(OmniCure Series 2000, Lumen Dynamics)用來交 聯水膠預聚物溶液，其輸出之光線波長介於320 nm-500 nm，可自行設定曝光時 間，照度則介於 0.2-40 W/cm2，可藉由原廠的 UV 照度計(OmniCure radiometer, R2000-2025, Lumen Dynamics)進行量測，該照度計之準確度為 5%，量測波長範 圍介於250 nm-1000 nm，量測功率介於 1-12000 mW。
動態 ELISA 讀盤機
本研究使用動態ELISA 讀盤機(Vmax, Molecular Devices)檢測實驗中細胞分 泌物經過ELISA 方法成色後的吸光值，並依照比色法計算出該分泌物之含量。