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(A) (B)
(C) (D)
(A) (B)
(C) (D)
圖一、異位性皮膚炎的建立
主要是以經皮致敏(Epicutaneous sensitization )的方式來建立。(A)先將老鼠背部的 毛剃除。(B)再以雞卵蛋白Ovalbumin (OVA)用量:100mg/ml、20ul/patch;加於貼布 膠帶(finn chambers)後貼附於老鼠背上。(C)(D)利用止血繃帶加以固定。以每天換 藥的方式，連續五天的貼皮致敏，之後須休息兩個禮拜，為一個循環，必須進行 三個循環。
-1 0 1 SCLFP、yeast:150 mg/ml 、200μl / mice
H2O: 200μl/ mice
PBS: 20μl/patch
OVA : 100mg/ml 、20μl/patch
-1 0 1 SCLFP、yeast:150 mg/ml 、200μl / mice
H2O: 200μl/ mice
PBS: 20μl/patch
OVA : 100mg/ml 、20μl/patch
圖二、異位性皮膚炎之誘導期實驗設計
圖三、偵測誘導期的浸潤淋巴結以OVA 刺激後分泌細胞激素的情形 利用ELISA 來測定有無以 OVA 刺激浸潤淋巴結的細胞，培養 48hr 後，所分泌的 細胞激素(A)IFN-γ。(B)IL-13。(C)IL-4。(D)IL-10。*代表統計學上 P 值<0.05，**
代表 P 值<0.001，***代表統計學上 P 值<0.0001。
圖四、偵測誘導期的浸潤淋巴結以OVA 刺激後細胞增生的情形 將老鼠犧牲後取出浸潤淋巴結，以有無加OVA 兩個條件進行細胞培養 48hr 後，
加入isotopes[H3]thymidine Incorporation 培養 16hr，以記數儀器進行放射線計數。
圖示以有加OVA 的細胞增生結果 cpm 值除以沒有以 OVA 培養的組別，所得到增 生的倍數，stimulation indexes=[H3]thymidine Incorporation (cpm) / unstimulated cells。*代表統計學上 P 值<0.05，**代表 P 值<0.001，***代表統計學上 P 值<0.0001。
***P=2.27E-08
**P=0.00043
***P=1.66E-05
***P=2.27E-08
**P=0.00043
***P=1.66E-05
***P=2.27E-08
**P=0.00043
***P=1.66E-05
圖五、異位性皮膚炎之作用期實驗設計 取serum, spleen, MLN
6 7 取serum, spleen, MLN
6 7
SCLFP、yeast: 150 mg/ml 、200 μl / mice H2O: 200 μl/ mice
PBS: 20 μl/patch
OVA : 100 mg/ml 、20 μl/patch
圖六、偵測作用期的老鼠血清中抗OVA 特異性抗體
利用ELISA 來測定老鼠血清中抗 OVA 特異性抗體在餵食豆類發酵產物後是否有 差異。以血清連續10 倍稀釋的方式來偵測 OD450 的值，並取 OD450 值為 1.0 以 內的結果來進行比較。(A)抗 OVA 特異性抗體 IgE。(B)抗 OVA 特異性抗體 IgG2a。
*代表統計學上 P 值<0.05，**代表 P 值<0.001，***代表統計學上 P 值<0.0001。
* P=0.04 ** P=0.0088
OVA-specific IgE OVA-specific IgG2a
(A) (B)
* P=0.04 ** P=0.0088
OVA-specific IgE OVA-specific IgG2a
* P=0.04 **** P=0.0088P=0.0088
OVA-specific IgE OVA-specific IgG2a
(A) (B)
圖七、作用期的異位性皮膚炎老鼠模式各組在外觀上的差異
在進行貼皮致敏三個循環後，老鼠犧牲前的皮膚狀況。分組如下: (A)Negative 組: 貼 膚致敏的藥劑為PBS，沒有進行餵食。(B)Positive 組: 貼膚致敏的藥劑為 OVA，
沒有進行餵食。(C)SCLFP 組: 貼膚致敏的藥劑為 OVA，進行豆類發酵產物的餵 食。(D)Yeast 組: 貼膚致敏的藥劑為 OVA，進行酵母菌的餵食。(E) H2O 組: 貼膚 致敏的藥劑為OVA，進行二次水的餵食。箭頭所指為明顯的紅腫傷口。
Negative Positive
SCLFP Yeast H2O
(A) (B)
(C) (D) (E)
Negative Positive
SCLFP
SCLFP YeastYeast H2OH2O
(A) (B)
(C) (D) (E)
圖八、偵測作用期的脾臟細胞以OVA 刺激後分泌細胞激素的情形 利用ELISA 來測定有無以 OVA 刺激脾臟細胞，培養 48hr 後，所分泌的細胞激素。
圖八、偵測作用期的脾臟細胞以OVA 刺激後分泌細胞激素的情形 利用ELISA 來測定有無以 OVA 刺激脾臟細胞，培養 48hr 後，所分泌的細胞激素。