石斛的組織培養
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(2) S23. Table 1. Effect of basal medium and cytokinins on cutting culture of Dendrobium moniliforme (L.) Sw. for explants after 30 days. Basal medium. Treatment (mg/L) Kin BA Z B5 25 0 0 B5 15 0 0 B5 10 0 0 B5 4 0 0 B5 2 0 0 B5 0.2 0 0 B5 0.1 0 0 B5 0 0 0 MS 0 1 0 MS 0 0.5 0 MS 25 0 0 MS 15 0 0 MS 5 0 0 MS 0.5 0 0 MS 0 0 0 MS 0 0 2 For each treatment, 10 cutting stems in test tube.. Average number of axillary shoots. Growth responses of axillary shoots. 0 1 2 2 3 3 0 0 1 0 0 2 2 0 0 0. 0 light yellow buds, no root light yellow buds, no root light yellow buds, root small buds, root small buds, root 0 0 green buds; black explant 0 0 green buds; black explant green buds; black explant 0 0 0. 6-Benzyladenine (BA) (KIN) [1,23]. (Dendrobium. moniliforme (L.) Sw.). Zeatin (Z). -Naphthalene Acetic Acid 0.3%. (NAA) (G-1. Hyponex 1 G-2. G-3. 0.5%. (. ) + 0.5. + 3%. B5 + 2.5 mg/LBA + 2 g/L. 100 mL. (0. Tween 20. 15%. +15%. mg/LBA + 2 g/L 30. 15%. 15%. +15% 75%. Kinetin. 1%. 2%. 6%). 3%. 4%. 1N HCl. ). pH. 5.7. 5%. 1N NaOH. 0.1. 15 lb/in2 (1.05 Kg/cm2). 121. 1.0 cm. 25. pH. 5.2. 0.1. 10 mL 23 (PLBs). ( ( 100 rpm 14. MS (Murashige & Skoog) [24] B5. [24]. 3%. 0.3%. 2,500. 2,500 Lux). Lux. G-3. 2. 2. ).
(3) Dendrobium moniliforme (L.) Sw.. S24. Table 2. Effect of 6-benzyladenine (BA) and naphthalene acetic acid (NAA) concentration on cutting stem culture of Dendrobium moniliforme (L.) Sw. for 40 days. B5 basal medium treatment (mg/L) NAA BA 10 0 5 0 2.5 0 0.5 0 0.02 0 10 0.5 5 0.5 2.5 0.5 0.5 0.5 0.02 0.5 10 2.5 5 2.5 2.5 2.5 0.5 2.5 0.02 2.5 10 5 5 5 2.5 5 0.5 5 0.02 5. Number of necrosis. Average length of axillary shoots (cm). Number of axillary shoots. 2 3 2 3 3 4 4 0 0 0 8 6 4 8 20 20 20 20 20 20. Stem. Root d. 2 3 2 3 3 4 4 0 0 0 8 6 4 8 20 20 20 20 20 20. Leaf. a. 0.0 1.5 0.0d 0.9bc 0.5ab 0.8bc a 0.6 1.0bc 0 0 d c 0.2 0.6 d 0.0 0.5c a 0.6 1.5a d 0.2 1.0bc 0 0 ab bc 0.5 1.0 bc 0.4 0.9bc 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 * Means with the same superscripts are not significantly different at 5% level by Dancan's multiple range test.. ( 14. 2,500 Lux. ). 25. 1.0ab 0.8bc 0.7c 1.0ab 0 a 1.5 bc 0.8 1.0ab 0.7c 0 ab 1.0 0.7c 0 0 0 0 0 0 0 0. 40. 40. Table 2 mg/L. NAA 5. NAA 2.5 mg/L BA 0.02 mg/L BA 0.5 mg/L. 10 mg/L. BA NAA 0.5 mg/L 1. B5. BA (0. 0.1. mg/L). MS. 5. 25 mg/L). 15. 0.2. 2. 4. BA (0 MSZ (0.5. 10. 25. 0.5. 1 mg/L). 1 B5Kin. 0.5. 1. 1.5. 2. 30. 1.5. Table 1 B5BA (0.2. 2 2. 0.5. 4 mg/L) 3. MSBA (5. 15 mg/L) G-1 B5. BA. G-2. G-3. 90 1. B5. BA (0.02 NAA (0. 0.5. 2.5. 150 14. g/L. 0.5. 2.5. 5 mg/L). 5. 10 mg/L). G-3 4. Table 3 4. 5.
(4) S25. Table 3. Effect of 15% potato aqueous extract, 15% banana aqueous extract, and 15% coconut water on plantlet strength of Dendrobium moniliforme (L.) Sw. for 90 days. Number of plants. Medium. Height of plant (cm). Average growth (cm). Number of die. Root. Leaf. c*. b. Stem. G-1 >1 6 20 1.5 1.0c 2.0 d c c G-1 <1 13 20 0.0 1.0 0.8 c c c G-2 >1 5 20 2.0 1.0 1.0 d c c G-2 <1 11 20 0.0 0.8 0.8 a a a G-3 >1 0 20 5.5 3.5 4.5 ab ab bc G-3 <1 2 20 3.5 2.0 3.0 G-1: 0.3% Hyponex 1 added with 15% potato aqueous extract, 0.5 mg/L BA, 3% sucrose and activated charcoal 2 g/L. G-2: 0.3% Hyponex 1 added with 15% banana aqueous extract, 0.5 mg/L BA, 3% sucrose and activated charcoal 2 g/L. G-3: 0.3% Hyponex 1 added with 15% potato aqueous extract, 15% banana aqueous extract, 15% coconut wate, 0.5 mg/L BA, 3% sucrose and activated charcoal 2 g/L. *Means with the same are not significantly different at 5% level by Dancan's multiple range test.. Table 4. Influence of acclimatisation on the survival of Dendrobium moniliforme (L.) Sw. for 30 days. Acclimatisation place. Number of plantlet transplants. Survival plants. 120 120. 103 (85%) 67 (55%). Greenhouse Temperature controlled room. (PLBs). (Fig. D) (PLBs). 0.5 1. (0.5. 1 2.5. B5BA 5. 10. 14. 25 mg/L). 40. (7,000 Lux). 1/2 MS. 12. B5BA 2.5 mg/L 1.5. (0. 1%. 2%. 3%. 4%. ). BA (2,000 Lux). B5BA 2.5 mg/L. (35 15. 5%. (26. ). 6%) 14. 40. 5% 3. (Fig. A). (. CMC89-PC-01). (Table 4, Figs. B, C) 1. 1977:899 2. 3.. 1995:114 2000:586-90.
(5) Dendrobium moniliforme (L.) Sw.. S26. A. B. C. D. Figure. The plantlets (Dendrobium moniliforme (L.) Sw.) were transferred to plastic cups, containing a mixture of cyantheaceae, scrap and small stones in 1:1 ratio and kept moist in greenhouse. A: Protocorm like bodies (PLBs). B: Acclimatization of plantlets at temperature controlled room. C: Acclimatization of plantlets in greenhouse. D: At greenhouse after one year.. 4. 1995 5. 1992;23:227-8 6. Talapatra SK, Bhaumik A, Talapatra B. Denfigenin, a diosgenin derivative from Dendrobium fimbriatum. Phytochemistry 1992;31:2431-4.. 11. Lee YH, Park JD, Baek NI, et al. In vitro and in vivo antitumoral phenanthrenes from the aerial parts of Dendrobium nobile. Planta Med 1995;61:178-80. 12. Miyazawa M, Shimamura H, Nakamura S, et al. Antimutagenic activity of gigantol from Dendrobium nobile. J Agric Food Chem 1997;45:2849-53. 13. 1995;26:7980. 7. 14. 1994;21:503-6 1991;23:97-9. 8. 1994;5:198-9 9. C h e n C C , Wu L G , K o F N , e t a l . Antiplatelet aggregation principles of Dendrobium loddigesii. J Nat Prod 1994;57:1271-4. 10. Veerra Ju P, Prakasa Rao NS, Jaganmohana Rao L, et al. Bibenzyls and phenanthrenoids of some species of Orchidaceae. Phytochemistry 1989;28:3031-4.. 15. 2000 16. Ma GX, LeBlance GA. The activity of erianin and chrysotoxine from Dendrobium chrysotoxum to reverse multidrug resistance in B16/n MDR-1 cells. J Chin Pharm Sci 1998;7:142-6..
(6) S27. 17.. 2000;25:23-4 22.Chung JD, Chun CK, Young YK, et al. Studies on 1995. 18. 2000;25:323-6 19. 2000;11:763-4 20. 1998;23:654-6 21.. aseptic culture of seeds in Dendrobium monile. . Effect of light and/or dark treatment and various compositions of media on germination of seeds and growth of seedlings. Jorunal of the Korean Society for Horticultural Science 1981;22:139-45. 23. Flora of Taiwan. 1996;5:845. 24.. 1998:20-5.
(7) S28. Tissue Culture of Dendrobium moniliforme (L.) Sw. 1. 1. 1. Jin-Bin Wu, Hui-Ya Ho, Wen-Ju Hu , Jung-Chun Liao , Chung-Chuan Chen , 2. Susumu Kitanaka 1. Institute of Pharmaceutical Chemistry, Institute of Chinese Pharmaceutical Sciences, China Medical 2. University, Taichung, Taiwan; College of Pharmacy, Nihon University, Chiba, Japan.. Objectives. Dendrobium moniliforme (L.) Sw. commonly known as shi-ho in Chinese, is a perennial herb indigenous to Japan. Dendrobium moniliforme (L.) Sw. contains alkaloids and polysaccharides and is used in traditional Chinese medicine. This study attempted to establish propagation of Dendrobium moniliforme (L.) Sw. Methods. We cultured cut stems of the plant on B5 medium (pH5.7 with suitable hormones at 23. 0.1) supplemented. 2 C. The stems were then transfered to G-3 medium for. 60 days. R e s u l t s . Two to three axillary shoots grew from cut stems cultured on medium containing B5 salts with 2.5 mg/L 6-benzyladenine and 0.5mg/L naphthalene acetic acid and 3% sucrose at 23. 2 C. The plantlets from the axillary shoots cultured on G-3. medium grew better and were more healthy. The plantlets (stem length 4.5 cm) were transfered to a plastic cup containing a mixture of cyantheaceae scrap and small stones in 1:1 ratio and kept moist. Over 85% of the plantlets survived and they grew to about 15 to 20 cm after one year in the greenhouse. Conclusions. Our tissue culture technique led to mass propagation of Dendrobium moniliforme and could resolve the deficiency of this herb on the traditional Chinese medicinal market. ( Mid Taiwan J Med 2003;8 Supplment:S22-8 ). Key words Dendrobium moniliforme, tissue culture, propagation of cutting stem, planting greenhouse. Received : March 28, 2003.. Revised : April 25, 2003.. Accepted : May 22, 2003. Address reprint requests to : Jin-Bin Wu, Institute of Pharmaceutical Chemistry, China Medical University, 91 Hsueh-Shih Road, Taichung 404, Taiwan, R.O.C..
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