DNA分子標誌資料之遺傳變異的歸群分析
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(2) (random amplified polymorphic DNA, RAPD) (amplified fragment length polymorphism, AFLP) DNA. ( (A). ) (marker loci) (operational taxonomic unit, OTU). DNA (restriction fragment length polymorphism, RFLP) (simple sequence repeat, SSR). DNA OTU (a=. b. c=. d=. ) (primer) dominant markers) ( (dominant markers). OTU. (co). OTU. (. ) ( 1. RAPD. OTU ) Jaccard. (B). 0 (. SSR. RFLP) (frequency) SSR RFLP. (0,1) (. AFLP). Jaccard =0.375. Nei &Li [0,1] a = a+b+c = 3 / ( 3 + 2 + 3 ). (. ). Nei & Li =2(3)/[2(3)+2+3]=0.545. 2a = 2a+b+c OTU. Nei & Li (a) Nei & Li (a). 26. (b,.
(3) analysis). c) Jaccard. Ward. (. (OTU) (a= ). d=. b. c=. (average linkage). ) ) Nei & Li. SSR. ( RFLP. (unweighted pair group method using arithmetic average, UPGMA) DNA. RAPD AFLP. Jaccard SSR. AFLP Nei & Li Gower Jaccard Sorensen. DNA Dice. OTU. Nei & Li (PCoA, principal coordinate analysis. DNA OTU (association matrix). MDS) (PCA, principal component analysis) PCA PCoA PCA. OTU. dendrogram) dendrogram). (cluster (ordination (C) (D). PCoA OTU (OTU). (cluster. 27.
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