行政院國家科學委員會專題研究計畫 期中進度報告
雙截切連續事件資料之分析(1/2)
計畫類別： 個別型計畫
計畫編號： NSC91-2118-M-002-003-
執行期間： 91 年 08 月 01 日至 92 年 07 月 31 日
執行單位： 國立臺灣大學公共衛生學院公共衛生學系
計畫主持人： 張淑惠
報告類型： 精簡報告
處理方式： 本計畫可公開查詢
中 華 民 國 92 年 6 月 2 日
Table 1. Effect of antibodies from rabbit antiserum against crude
progesterone receptor proteins on sperm motility (means SD).
0 20 40 60 80 100 500 1000 2000 4000 8000 16000 M ot il it y %
Control Male 1 Male 2 Female 1 Female 2 Activated serum 8010 a 4023b 2918b 23c 157c Inactivated serum* 828 6515 8017 7513 6917
*
Inactived serum: serum were heat-treated at 56℃for 30 min to inactivate complement.a,b,c
different superscripts are significantly different (p < 0.05 to p < 0.001) and same superscripts are non -significant (p > 0.05).
Figure 3. Effects of dilution of rabbit anti-PRs antiserum (female 1) on sperm motility in the presence of guinea pig complement (55ng/mL). Bars represent mean
a,b
different superscripts are significantly different (p < 0.05) and same superscripts are non-significant (p > 0.05).
0 20 40 60 80 100 5 5 11 0 2 20
guinea pig complement (ng/mL)
M ot il ti y % complement inactived serum+complement 0 5 10 15 20 25 30 35
male 1 female 1 female 2
B
-3
-4
2-
1-A
A′
B′
*
b
b
ab
a
a
*
*
*
Figure 1. Western blot indicating the specific proteins recognized by rabbit antiserum against crude progesterone receptor proteins. A & B:
d i f f e r e n t f r a c t i o n a l m e m b r a n e p r o t e i n s p u r i f i e d f r o m progesterone-conjugated affinity column were separated by SDS-PAGE.
A′& B′respectively corresponding to A & B, but were further processed western blot with rabbit antiserum. Th e major apparent molecular mass (app. 72-80 kDa, protein 1) were revealed by rabbit antiserum combined with peroxidase-conjugated goat anti-rabbit IgG. However, protein 2 (127 kDa), 3 (64 kDa) and 4 (125 kDa) are others major proteins that didn’t recognized by rabbit antiserum.
Figure 2. Immnunoreactivity of antibodies from rabbit antiserum
against pig crude progesterone receptor proteins using indirect immunofluorescene (A). Intense fluorescence had been observed over the whole acrosomal region. Control rabbit serum did not react with sperm. Th e con for mation of th e same sperm with intact plasma membrane i s m a d e vi si bl e un d er a l i gh t m i cr oscop e ( B) . No fluorescence was observed when 1st antibody was omitted.
A
Figure 4. Effect of inactived rabbit anti-PRs antiserum (female 1, 1:10)
combined with guinea pig complement on sperm motility. Bars represent mean SD.*
p < 0.05 compared with the control, which had the same complement concentration as the treatment but without inactivated antiserum.
Figure 5. Effect of different inactived rabbit antisera (male 1,
female 2 & 3) against crude PR proteins on sperm
bin ding assa y. Sperm wer e pr ein cubated for 4 h for capacitation, and then co-incubated rabbit antiserum (1:10) or
not for 1 h. After incubation, 3000 motile sperm were added to medium containing salted piglet oocyte for 1 h. Bars represent mean SD. *
