1. 本研究顯示，在所評估之濃度 23 μg/mL EMA 及 PMA，於螢光顯微鏡下觀察，
EMA 或 PMA 皆可進入受熱細菌的細胞膜內，但 EMA 也會進入完整細胞膜 內，而 PMA 較無此情況。
2. 本研究顯示，在所評估之各濃度 EMA 及 PMA 中， 1.5 μg/mL PMA 可有效 抑制受熱組細菌於 qPCR 放大，且在此濃度下，不會對活性細菌造成 qPCR 定量濃度之干擾。因此以1.5 μg/mL PMA 濃度做為結合 qPCR 定量空氣中活 性細菌之最佳條件。
3. 進一步以最佳核酸染劑條件 1.5 μg/mL PMA 評估細菌濃度定量範圍，結果顯 示，最佳線性範圍介於 1×104 ~ 1×1010 cfu/mL。
4. 將開發之方法進行職業場所空氣採樣之樣本分析，量測場所包括畜牧場、家 禽舍、木材行、水稻田、蔬菜田及醫療院所。結果顯示所開發之 PMA/qPCR 方法確實可作為定量空氣中活性細菌之分析方法。
108
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119 concentration
(cells/m3)
total cell concentration
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total cell concentration
(cells/m3)
120
ntc 31.92 1.18 118.20 236.39 472.78 2.67 2.14 137.34 183.12 ntc 33.96 0.60 60.18 120.36 240.71 2.38 1.83 68.34 91.12 ntc 31.65 1.26 125.87 251.75 503.50 2.70 2.17 146.58 195.44 ntc 31.64 1.26 126.16 252.32 504.63 2.70 2.17 146.92 195.89 ntc 30.16 1.68 168.25 336.50 673.00 2.83 2.30 197.86 263.82 ntc 32.88 0.91 90.89 181.79 363.57 2.56 2.02 104.68 139.57 ntc 30.11 1.70 169.67 339.34 678.69 2.83 2.30 199.59 266.12 ntc 30.98 1.45 144.93 289.86 579.72 2.76 2.23 169.58 226.10 ntc 30.19 1.67 167.40 334.79 669.59 2.83 2.29 196.83 262.44 ntc 31.13 1.41 140.66 281.33 562.65 2.75 2.22 164.42 219.22 ntc 30.85 1.49 148.63 297.25 594.50 2.77 2.24 174.05 232.07 ntc 32.26 1.09 108.53 217.05 434.10 2.64 2.10 125.74 167.65 ntc 31.74 1.23 123.31 246.63 493.26 2.69 2.16 143.50 191.33 ntc 31.93 1.18 117.91 235.82 471.64 2.67 2.14 137.00 182.66 ntc 31.76 1.23 122.75 245.49 490.98 2.69 2.15 142.81 190.41 ntc 30.2 1.67 167.11 334.22 668.45 2.83 2.29 196.48 261.97 ntc 30.06 1.71 171.09 342.19 684.37 2.84 2.30 201.32 268.43 ntc 30.39 1.62 161.71 323.42 646.83 2.81 2.28 189.92 253.22 ntc 30.89 1.47 147.49 294.98 589.95 2.77 2.24 172.67 230.23
ntc 30.3 1.64 164.27 328.54 657.07 2.82 2.29 193.02 257.37
121
cell numbers in the rest aliquot copies/sample concentration
(cells/m3)
Viable cell concentration
(cells/m3) mean
Viable cell concentration
(cells/m3)
122
ntc 31.65 1.26 125.87 251.75 503.50 2.70 2.07 117.51 156.68 ntc 31.64 1.26 126.16 252.32 504.63 2.70 2.07 117.80 157.06 ntc 30.16 1.68 168.25 336.50 673.00 2.83 2.21 160.99 214.65 ntc 32.88 0.91 90.89 181.79 363.57 2.56 1.92 82.54 110.05 ntc 30.11 1.70 169.67 339.34 678.69 2.83 2.21 162.47 216.62 ntc 30.98 1.45 144.93 289.86 579.72 2.76 2.14 136.93 182.57 ntc 30.19 1.67 167.40 334.79 669.59 2.83 2.20 160.10 213.47 ntc 31.13 1.41 140.66 281.33 562.65 2.75 2.12 132.56 176.75 ntc 30.85 1.49 148.63 297.25 594.50 2.77 2.15 140.72 187.63 ntc 32.26 1.09 108.53 217.05 434.10 2.64 2.00 100.04 133.39 ntc 31.74 1.23 123.31 246.63 493.26 2.69 2.06 114.92 153.22 ntc 31.93 1.18 117.91 235.82 471.64 2.67 2.04 109.46 145.95 ntc 31.76 1.23 122.75 245.49 490.98 2.69 2.06 114.34 152.46 ntc 30.2 1.67 167.11 334.22 668.45 2.83 2.20 159.81 213.08 ntc 30.06 1.71 171.09 342.19 684.37 2.84 2.21 163.94 218.59 ntc 30.39 1.62 161.71 323.42 646.83 2.81 2.19 154.21 205.61 ntc 30.89 1.47 147.49 294.98 589.95 2.77 2.14 139.55 186.07
ntc 30.3 1.64 164.27 328.54 657.07 2.82 2.20 156.86 209.15
123
Primer 的選擇?與古生細菌僅差一個 base pair，
primer 不一定就黏不到。
124
125