本研究對地耳草進行基原鑑定，並利用植物組織培養技術建立植 株之大量繁殖的系統，並進行癒合組織誘導、繼代培養及植株再生，
探討不同培養條件對地耳草生成quercetin 之影響，希望藉由此研究作 為地耳草鑑定及開發大量繁殖之途徑，以提供市場所需並進行後續研 究之參考。茲將試驗結果總結如下：
一、地耳草基原鑑定
由地耳草形態具幼枝四角形，老莖4 縱條紋，單葉對生，無葉柄，
基部心形抱莖，葉面散生半透明腺點，聚繖花序頂生，花瓣 5，黃色 等特徵可鑑別出其原植物；顯微組織圖中可觀察到其莖四方形，下皮 細胞多充滿棕色內含物，內皮層1 列明顯，中央髓部大多中空，導管 具螺旋紋及網紋等組織特徵；以其 ITS 序列，和已登錄於 NCBI 的地 耳草序列作比對，結果相似度達99%以上，此 ITS 序列也可作為地耳 草分子生物鑑定之依據。
二、地耳草大量繁殖
將培殖體培養於含1 mg/L ZT 之培養基中能誘導最多芽體，且以 莖節的誘導率最高，培養4 週後再繼代至含 1% 蔗糖之 WPM 培養基，
能長出最多的不定芽。將不定芽繼代至含5% 蔗糖之 1/2 MS 培養基 中，並添加1 mg/L 之 IAA 或 kinetin，各能得到最健壯的地上部及最 健全的根系，並能成功馴化至野外。
三、地耳草癒合組織之誘導、繼代培養與植株再生
以MS 基礎鹽類含 1 mg/L BA 之固體培養基中，莖節培殖體可獲 得最高誘導率，而最佳繼代培養條件為MS 基礎鹽類添加 1 mg/L BA、
3% sucrose、0.3% gelrite 及 pH 5.70 ± 0.01 之培養基，在 25 ± 1 ℃之 恆溫下進行暗培養，15 天繼代一次為宜。降低 BA 濃度會誘導癒合組 織長出不定芽，以0.1 mg/L BA 能使癒合組織再生最多芽體，在將之
繼代至不含生長調節劑之1/2 MS 培養基並培養於光照環境，能使不 定芽抽長並發根，亦達到了大量繁殖的目的。
四、地耳草成分之測定
地耳草癒合組織及植株培養於不同培養基條件下，經冷凍乾燥研 磨成粉，以 70%甲醇萃取，經 HPLC 檢測 quercetin 含量。試驗結果顯 示，地耳草的癒合組織培養於離子濃度較低的WPM 培養基、1/4 MS、
1/2 MS 培養基較利於 quercetin 的生成；3%蔗糖或葡萄糖利於地耳草 癒合組織生長，也利於其 quercetin 的生成，而果糖不利於地耳草癒合 組織生長及quercetin 的生成，培養 5 天時 quercetin 的含量最高； N6 培養基及果糖不適合地耳草植株生長，quercetin 含量反而最高，添加 auxins 類生長調節劑有益於地耳草植株發根及其 quercetin 之產生。
附 錄
培養基之組成
Hildebrandt, 1972 )mg/L
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