參考文獻 - 以密閉式藻類毒性試驗方法評估一級炔丙基醇類之毒性

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附錄一:原始數據

Primary propargylic alcohols

實驗毒物 : 2-propyn-1-ol

mg/L mg/L mg/L cells/ml mg/L μspecific μrelative ( growth rate) (Biomass) (DO)

Control 1.22 8.94 328600 7.72 1.54340 1 0 0 0

IR : Inhibition rate

Biomass : Yield f (Final yield based on cell density)

實驗毒物 : 2-butyn-1-ol

mg/L mg/L mg/L cells/ml mg/L μspecific μrelative ( growth rate) (Biomass) (DO)

Control 1.7 7.93 274800 6.23 1.45400 1 0 0 0

IR : Inhibition rate

Biomass : Yield f (Final yield based on cell density)

實驗毒物 : 2-butyn-1,4-diol 初始細胞密度

(cells/mL) : 15000

MCV (μm3) : 39.2 D (μm) : Initial pH : 7.46 EDTA(%) : 0 T(℃) : 24.1 Test duration : 48-h

Conc Initial DO Final DO Final cells Delta DO IR IR IR

mg/L mg/L mg/L cells/ml mg/L μspecific μrelative

( growth rate) (Biomass) (DO)

IR : Inhibition rate

Biomass : Yield f (Final yield based on cell density)

mg/L mg/L mg/L cells/ml mg/L μspecific μrelative

( growth rate) (Biomass) (DO)

Control 0.8 8.42 318300 7.62 1.52747 1 0 0 0

2.41 0.98 8.21 316700 7.23 1.52495 0.99835 0.00165 0.00528 0.05118 4.83 0.87 7.55 305300 6.68 1.50662 0.98635 0.01365 0.04286 0.12336 9.66 1.11 8.72 278300 7.61 1.46032 0.95604 0.04396 0.13188 0.00131 19.32 1.02 7.74 242200 6.72 1.39086 0.91056 0.08944 0.25091 0.11811 38.63 1.28 6.64 184700 5.36 1.25534 0.82184 0.17816 0.44049 0.29659 77.27 1.43 5.49 109400 4.06 0.99348 0.65041 0.34959 0.68876 0.46719 154.53 1.94 3.02 43900 1.08 0.53693 0.35152 0.64848 0.90471 0.85827

Control 1.04 8.44 318500 7.4 1.52779 1 0 0 0

2.41 1.13 8.19 316900 7.06 1.52527 0.99835 0.00165 0.00527 0.04595 4.83 1.03 7.86 304200 6.83 1.50482 0.98497 0.01503 0.04712 0.07703 9.66 1.26 8.2 278900 6.94 1.46140 0.95655 0.04345 0.13048 0.06216 19.32 1.12 7.77 241300 6.65 1.38900 0.90916 0.09084 0.25437 0.10135 38.63 1.44 6.79 183900 5.35 1.25317 0.82025 0.17975 0.44349 0.27703 77.27 1.41 5.52 109900 4.11 0.99576 0.65177 0.34823 0.68731 0.44459 154.53 1.95 3.16 45400 1.21 0.55373 0.36244 0.63756 0.89984 0.83649

Control 1.02 8.39 317900 7.37 1.52684 1 0 0 0

2.41 1.11 8.16 316400 7.05 1.52448 0.99845 0.00155 0.00495 0.04342 4.83 1.05 7.79 304800 6.74 1.50580 0.98622 0.01378 0.04325 0.08548 9.66 1.24 8.14 280300 6.9 1.46391 0.95878 0.04122 0.12413 0.06377 19.32 1.18 7.68 242600 6.5 1.39168 0.91148 0.08852 0.24860 0.11805 38.63 1.39 6.76 183200 5.37 1.25126 0.81951 0.18049 0.44470 0.27137 77.27 1.42 5.48 108700 4.06 0.99027 0.64857 0.35143 0.69066 0.44912 154.53 1.92 3.09 44600 1.17 0.54484 0.35684 0.64316 0.90228 0.84125

Control 0.95333 8.41667 318233.33 7.46333 1.52737 1 0 0 0

2.41 1.07333 8.18667 316666.67 7.11333 1.52490 0.99838 0.00162 0.00517 0.04690 4.83 0.98333 7.73333 304766.67 6.75000 1.50575 0.98585 0.01415 0.04441 0.09558 9.66 1.20333 8.35333 279166.67 7.15000 1.46188 0.95712 0.04288 0.12883 0.04198 19.32 1.10667 7.73000 242033.33 6.62333 1.39051 0.91040 0.08960 0.25129 0.11255 38.63 1.37000 6.73000 183933.33 5.36000 1.25326 0.82054 0.17946 0.44289 0.28182 77.27 1.42000 5.49667 109333.33 4.07667 0.99317 0.65025 0.34975 0.68891 0.45377 154.53 1.93667 3.09000 44633.33 1.15333 0.54517 0.35693 0.64304 0.90228 0.84547

IR : Inhibition rate

實驗毒物 : 2-hexyn-1-ol 初始細胞密度

(cells/mL) : 15000

MCV (μm3) : 40.7 D (μm) : Initial pH : 7.54 EDTA(%) : 0

T(℃) : 23.7 Test duration : 48-h

Conc Initial DO Final DO Final cells Delta DO IR IR IR

mg/L mg/L mg/L cells/ml mg/L μspecific μrelative

( growth rate) (Biomass) (DO)

Control 1.27 6.54 271300 5.27 1.44759 1 0 0 0

2.43 1.23 6.43 270500 5.2 1.44611 0.99898 0.00102 0.00312 0.01328 4.86 1.24 6.34 249000 5.1 1.40470 0.97037 0.02963 0.08701 0.03226 9.72 1.31 6.28 215600 4.97 1.33269 0.92063 0.07937 0.21732 0.05693 19.43 1.33 5.75 186600 4.42 1.26046 0.87073 0.12927 0.33047 0.16129 38.87 1.34 5.15 129500 3.81 1.07782 0.74456 0.25544 0.55326 0.27704 77.73 1.56 4.06 86000 2.5 0.87315 0.60317 0.39683 0.72298 0.52562 155.47 2.41 3.23 31100 0.82 0.36458 0.25185 0.74815 0.93718 0.84440

Control 1.22 6.52 271600 5.3 1.44814 1 0 0 0

2.43 1.24 6.39 270300 5.15 1.44574 0.99834 0.00166 0.00507 0.02830 4.86 1.25 6.32 248600 5.07 1.40390 0.96945 0.03055 0.08963 0.04340 9.72 1.27 6.25 214700 4.98 1.33060 0.91883 0.08117 0.22175 0.06038 19.43 1.32 5.66 187200 4.34 1.26206 0.87151 0.12849 0.32892 0.18113 38.87 1.34 5.22 129800 3.88 1.07897 0.74507 0.25493 0.55261 0.26792 77.73 1.48 4.12 85400 2.64 0.86965 0.60053 0.39947 0.72564 0.50189 155.47 2.39 3.18 31600 0.79 0.37255 0.25726 0.74274 0.93531 0.85094

Control 1.2 6.53 271400 5.33 1.44777 1 0 0 0

2.43 1.24 6.4 270400 5.16 1.44593 0.99873 0.00127 0.00390 0.03189 4.86 1.31 6.34 248100 5.03 1.40289 0.96900 0.03100 0.09087 0.05629 9.72 1.3 6.23 215900 4.93 1.33338 0.92099 0.07901 0.21646 0.07505 19.43 1.27 5.78 185300 4.51 1.25696 0.86821 0.13179 0.33580 0.15385 38.87 1.35 5.19 128900 3.84 1.07549 0.74286 0.25714 0.55577 0.27955 77.73 1.49 4.17 85800 2.68 0.87198 0.60229 0.39771 0.72387 0.49719 155.47 2.38 3.21 30900 0.83 0.36135 0.24959 0.75041 0.93799 0.84428

Control 1.23000 6.53000 271433.33 5.30000 1.44783 1 0 0 0

2.43 1.23667 6.40667 270400.00 5.17000 1.44593 0.99868 0.00132 0.00403 0.02453 4.86 1.26667 6.33333 248566.67 5.06667 1.40383 0.96961 0.03039 0.08917 0.04403 9.72 1.29333 6.25333 215400.00 4.96000 1.33222 0.92015 0.07985 0.21851 0.06415 19.43 1.30667 5.73000 186366.67 4.42333 1.25983 0.87015 0.12985 0.33173 0.16541 38.87 1.34333 5.18667 129400.00 3.84333 1.07743 0.74417 0.25583 0.55388 0.27484 77.73 1.51000 4.11667 85733.33 2.60667 0.87159 0.60200 0.39800 0.72416 0.50818 155.47 2.39333 3.20667 31200.00 0.81333 0.36616 0.25290 0.74708 0.93683 0.84654

IR : Inhibition rate

Biomass : Yield f (Final yield based on cell density)

實驗毒物 : 2-heptyn-1-ol

mg/L mg/L mg/L cells/ml mg/L μspecific μrelative ( growth rate) (Biomass) (DO)

Control 1.15 5.23 253400 4.08 1.41346 1 0 0 0

IR : Inhibition rate

Biomass : Yield f (Final yield based on cell density)

實驗毒物 : 2-octyn-1-ol

mg/L mg/L mg/L cells/ml mg/L μspecific μrelative ( growth rate) (Biomass) (DO)

Control 2.21 7.01 232900 4.8 1.37128 1 0 0 0

IR : Inhibition rate

Biomass : Yield f (Final yield based on cell density)

初始細胞密度

mg/L mg/L mg/L cells/ml mg/L μspecific μrelative ( growth rate) (Biomass) (DO)

Control 1.09 8.31 290600 7.22 1.48195 1 0 0 0

IR : Inhibition rate

Biomass : Yield f (Final yield based on cell density)

實驗毒物 : 2-decyn-ol

mg/L mg/L mg/L cells/ml mg/L μspecific μrelative ( growth rate) (Biomass) (DO)

Control 1.26 8.86 263600 7.6 1.43319 1 0 0 0

IR : Inhibition rate

Biomass : Yield f (Final yield based on cell density)

Primary homopropargylic alcohols

mg/L mg/L mg/L cells/ml mg/L μspecific μrelative ( growth rate) (Biomass) (DO)

Control 1.37 8.72 302600 7.35 1.50218 1 0 0 0

IR : Inhibition rate

Biomass : Yield f (Final yield based on cell density)

實驗毒物 : 3-pentyn-1-ol

mg/L mg/L mg/L cells/ml mg/L μspecific μrelative ( growth rate) (Biomass) (DO)

Control 1.65 6.09 257800 4.44 1.42207 1 0 0 0

IR : Inhibition rate

Biomass : Yield f (Final yield based on cell density)

實驗毒物 : 3-hexyn-1-ol

mg/L mg/L mg/L cells/ml mg/L μspecific μrelative ( growth rate) (Biomass) (DO)

Control 1.43 5.59 235400 4.16 1.37662 1 0 0 0

IR : Inhibition rate

Biomass : Yield f (Final yield based on cell density)

實驗毒物 : 3-heptyn-1-ol

mg/L mg/L mg/L cells/ml mg/L μspecific μrelative ( growth rate) (Biomass) (DO)

Control 1.58 9.43 281200 7.85 1.46551 1 0 0 0

IR : Inhibition rate

Biomass : Yield f (Final yield based on cell density)

實驗毒物 : 3-octyn-1-ol 初始細胞密度

(cells/mL) : 15000

MCV (μm3) : 43.3 D (μm) : Initial pH : 7.55 EDTA(%) : 0 T(℃) : 23.2 Test duration : 48-h

Conc Initial DO Final DO Final cells Delta DO IR IR IR

mg/L mg/L mg/L cells/ml mg/L μspecific μrelative

( growth rate) (Biomass) (DO)

IR : Inhibition rate

Biomass : Yield f (Final yield based on cell density)

實驗毒物 : 3-nonyn-1-ol

mg/L mg/L mg/L cells/ml mg/L μspecific μrelative ( growth rate) (Biomass) (DO)

Control 1.09 9.81 403100 8.72 1.64557 1 0 0 0

IR : Inhibition rate

Biomass : Yield f (Final yield based on cell density)

實驗毒物 : 3-decyn-1-ol

mg/L mg/L mg/L cells/ml mg/L μspecific μrelative ( growth rate) (Biomass) (DO)

Control 1.22 9.29 345200 8.07 1.56804 1 0 0 0

IR : Inhibition rate

附錄二:劑量反應曲線圖

Primary propargylic alcohols

0.00 0.00 0.01 0.10 1.00 10.00 100.00 1000.00 2-propyn-1-ol conc. (mg/L)

Inhibition Rate

DO. data

Final Yield. data Growth Rate. data

Fig 1 The Dose-response Curve of 2-propyn-1-ol

0.00 0.00 0.01 0.10 1.00 10.00 100.00 1000.00 2-butyn-1-ol conc. (mg/L)

Inhibition Rate

DO. data

Final Yield. data Growth Rate. data

Fig 2 The Dose-response Curve of 2-butyn-1-ol

0.00 0.00 0.10 10.00 1000.00 100000.00

2-butyn-1,4-diol conc. (mg/L)

Inhibition Rate

DO. data

Final Yield. data Growth Rate. data

Fig 3 The Dose-response Curve of 2-butyn-1,4-diol

0.00 0.00 0.01 0.10 1.00 10.00 100.00 1000.0 0

Final Yield. data Growth Rate. data

Fig 4 The Dose-response Curve of 2-pentyn-1-ol

0.00 0.00 0.01 0.10 1.00 10.00 100.00 1000.0 0

Final Yield. data Growth Rate. data

Fig 5 The Dose-response Curve of 2-hexyn-1-ol

0.00 0.00 0.10 10.00 1000.00 100000.00

2-heptyn-1-ol conc. (mg/L)

Inhibition Rate

DO. data

Final Yield. data Growth Rate. data

Fig 6 The Dose-response Curve of 2-heptyn-1-ol

0.00 0.00 0.01 0.10 1.00 10.00 100.00 1000.0 0

Final Yield. data Growth Rate. data

Fig 7 The Dose-response Curve of 2-octyn-1-ol

0.00 0.00 0.01 0.10 1.00 10.00 100.00 1000.0 0

Final Yield. data Growth Rate. data

Fig 8 The Dose-response Curve of 2-nonyn-1-ol

0.00 0.20 0.40 0.60 0.80 1.00 1.20

0.00 0.01 0.10 1.00 10.00 100.00 1000.00 2-decyn-ol conc. (mg/L)

Inhibition Rate

DO. data

Final Yield. data Growth Rate. data

Fig 9 The Dose-response Curve of 2-decyn-1-ol

Primary homopropargylic alcohols

0.00 0.10 10.00 1000.00 100000.00 10000000.

00 3-butyn-ol conc. (mg/L)

Inhibition Rate

DO. data

Final Yield. data Growth Rate. data

Fig 10 The Dose-response Curve of 3-butyn-1-ol

0.00 0.00 0.10 10.00 1000.00 100000.00

3-pentyn-ol conc. (mg/L)

Inhibition Rate

DO. data

Final Yield. data Growth Rate. data

Fig 11 The Dose-response Curve of 3-pentyn-1-ol

0.00 0.00 0.01 0.10 1.00 10.00 100.00 1000.0 0

Final Yield. data Growth Rate. data

Fig 12 The Dose-response Curve of 3-hexyn-1-ol

0.00 0.00 0.10 10.00 1000.00 100000.00

3-heptyn-ol conc. (mg/L)

Inhibition Rate

DO. data

Final Yield. data Growth Rate. data

Fig 13 The Dose-response Curve of 3-heptyn-1-ol

0.00 0.00 0.01 0.10 1.00 10.00 100.00 1000.0 0

Final Yield. data Growth Rate. data

Fig 14 The Dose-response Curve of 3-octyn-1-ol

0.00 0.00 0.01 0.10 1.00 10.00 100.00 1000.00 3-nonyn-1-ol conc. (mg/L)

Inhibition Rate

DO. data

Final Yield. data

Growth Rate. data

0.00 0.20 0.40 0.60 0.80 1.00 1.20

0.00 0.01 0.10 1.00 10.00 100.00 3-decyn-1-ol conc. (mg/L)

Inhi bi tion R at e

DO. data

Final Yield. data Growth Rate. data

Fig 16 The Dose-response Curve of 3-decyn-1-ol

在文檔中以密閉式藻類毒性試驗方法評估一級炔丙基醇類之毒性 (頁 128-160)