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胜肽包覆奈米粒子之合成及螢光免疫分析上之應用

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摘要

我們利用能與硫化鋅晶體產生結合能力的生肽分子,來置換硒化 鎘/硫化鋅奈米粒子表面的有機分子,而合成出具有高光學穩定性、 水溶性的奈米粒子。不同尺寸的奈米粒子經由此官能基轉換之後仍保 有其原本的吸收、螢光光學性質,且奈米粒子的粒徑大小並無明顯的 增減,而粒子與粒子間的分散性亦相當良好,顯示出這樣的水溶性奈 米粒子,非常適合於生物系統上的應用。最後我們將晶體表面上的生 肽分子,利用生物連接試劑與抗體作進一步的結合,並將此標定有半 導體奈米粒子的抗體進行螢光免疫檢測,實驗結果證實了該半導體奈 米粒子對抗體活性並無明顯的影響,因此這樣的奈米粒子-抗體連結 方式,可被期望作為一種生物檢測、標定的有效工具之一。

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Abstract

We utilized the peptide molecules capping ZnS crystal surface to exchange the organic ligand onto CdSe/ZnS core-shell nanoparticle, that resulted in highly fluorescent, water-soluble one. Various sizes of

particles via surface functional group exchange could retain their original morphology and optical property such as uv-vis absorption, and

fluorescent. To covalently attach the peptide-capped nanoparticles to biological macromolecules, we used cross-linker reagent coupling the nanoparticles and antibodys. The particles-labeled antibodys were employed in fluoroimmunoassays, and the result demonstrated that the particle-antibody conjugation would not affect the activity of protein. These peptide-capped nanoparticles promise to be a versatile tool for probing of biological systems and biolabeling.

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