英文關鍵字 ischemic stroke；MCAO；Tao Hong Si Wu TANG

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• 系統編號 RG9712-0065

• 計畫中文名稱中藥傳統方劑桃紅四物湯預防缺血性腦中風之療效評估

• 計畫英文名稱 --

• 主管機關行政院衛生署 • 計畫編號 CCMP97-RD-008

• 執行機構台北醫學大學醫學研究所

• 本期期間 9702 ~ 9712

• 報告頁數 25 頁 • 使用語言中文

• 研究人員許準榕；謝政穎；；；； Joen-Rong Sheu；cheng-ying, hsieh；；；；

• 中文關鍵字缺血性中風；中腦動脈血管阻塞/再灌流模式；桃紅四物湯；；；；；

• 英文關鍵字 ischemic stroke；MCAO；Tao Hong Si Wu TANG；；；；；

• 中文摘要

腦血管中風是血管性疾病致死及發病的主要原因之ㄧ，影響人類健康甚鉅，但由於其突發性，其治療藥物的發展，一直沒有很大的進展；中醫藥傳統方劑在腦血管中風的臨床治療上使用已久，然其研究的完善性及科學性仍嫌不足，也較不易令人信服。本計劃之目的在利用完善的研究模式，來探討中藥傳統方劑桃紅四物湯對缺血性腦中風之療效。本計畫利用大鼠之中腦動脈血管阻塞/再灌流模式以及小鼠之螢光染料引發腸繫膜血管血栓之實驗模式，來評估桃紅四物湯治療缺血性腦中風所造成腦部傷害的效果，並利用西方墨點法更深入的探討桃紅四物湯預防腦部傷害的相關因子的機轉，以期了解桃紅四物湯療效的分子作用機制。研究結果發現桃紅四物湯(1.2 及 2.4 g/kg)能有效的減少 MCAO 手術所造成之腦部傷害，且有抗血栓及抑制膠原蛋白引發之血小板凝集的作用，並會抑制 HIF-1、

TNF-及 iNOS 的表現。其腦部保護作用可能經由其抗血栓能力及抑制 HIF-1的表現進而影響 TNF-及 iNOS 的產生而減少腦細胞死亡進而達到保護腦部的效果。

• 英文摘要

Stroke is a main mortal cause of vascular diseases, and affects human health critically. Unfortunately, the therapy of ischemic stroke is still not good enough because stroke is unpredictable. Traditional Chinese medicine formula is used for stoke therapy for a long time, but its effects and mechanisms are still unconvincing. The aim of this study is to investigate the protective effect of Tao Hong Si Wu TANG on ischemic stroke through completed study models.This study will utilize middle cerebral artery occlusion (MCAO) model to evaluate the effect of Tao Hong Si Wu TANG on MCAO induced brain injury in rat. We also use fluorescein sodium induced thrombosis model to detect the anti-thrombosis function of Tao Hong Si Wu TANG in mice. Furthermore, we will investigate the molecular mechanisms of Tao Hong Si Wu TANG through

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western blotting. This study will estimate the effects of Tao Hong Si Wu TANG on stroke completely.The results show that Tao Hong Si Wu TANG (1.2 and 2.4 g/kg) could reduce MCAO-induced brain injury, and had anti-thrombus and anti-aggregation effects. Tao Hong Si Wu TANG also could inhibit MCAO-induced HIF-1、TNF- and iNOS expression. The protective effect of Tao Hong Si Wu TANG on MCAO-induced brain injury may through its anti-thrombus effect and the inhibition of HIF-1expression.