人參及人參皂苷 ; 於純系小鼠之馬兜鈴酸腎炎模型的藥效評估
馬兜鈴酸 (aristolochic acid, AA) 在中草藥引起的腎病變中扮演重要 的角色。本研究的目的為藉由投予 AA 引起馬兜鈴酸腎病變 (aristolo chic acid nephropathy, AAN) ，以評估人參 (ginseng extrat, GE) 及其 成份 ginsenoside Rb1 、 Rd 及 Rg1 對 AAN 的改善效果。
給予純系小鼠 C3H/He (6 week-old male) 3.0 μg/mL AA 當飲用水，連 續 56 天，之後治療組分別經口投予 GE (125 、 250 、 500 mg/kg) 或 純成份 ginsenoside (Rb1 、 Rd 、 Rg1) 5 mg/kg 連續 14 天，對照組給 予等量蒸餾水， Normal 組則全程給予蒸餾水。
藉由測定尿蛋白，尿中 N-acetyl-beta-D-glucosaminidase (NAG) 與血 中 blood urea nitrogen (BUN) 及 creatinine ，以評估小鼠腎功能；腎組 織使用 PAS 染色觀察病理組織改變，並進行免疫螢光染色 (TGF-β， MMP-9 ， HGF) ，以辨識損傷部位之特異性抗原。
實驗結果顯示，投予 GE 250 mg/kg ， ginsenoside Rb1, Rg1 之治療組 的尿蛋白、 NAG 、 BUN 、 SCr 及血糖值都有降低；組織學及免疫螢 光染色觀察發現腎組織損傷的情形皆有緩解。根據以上結果發現，人 參濃縮劑部分， GE 250 的療效最好，其次依序為 GE 500 及 GE 125 。 人參皂苷部分，有效程度依序為 Rg1 、 Rb1 、 Rd 。Effects of ginseng and ginsenosides on aristolochic acid-induced nephropathy in inbred mice
Aristolochic acid (AA) has been demonstrated to play a crucial role in Chinese herbs nephropa thy. The purpose of this study was to evaluate the therapeutic effect of ginseng extrat (GE) and its active component, ginsenoside (GS), on AA-induced nephropathy.
AA was dissolved in distilled water (3μg/ml) as drinking water to C3H/He mice (6 week-old male) for 56 days. The treatment groups were administered orally with GE (125, 250, 500 mg/
kg) or ginsenosides (Rb1, Rd, Rg1 5 mg/kg) once daily for 14 days. The control group was ad ministered with distilled water. The normal group was only administered with distilled water t hroughout the experiment.
Urine protein (UP), urine N-acetyl-beta-D-glucosaminidase (NAG), blood urea nitrogen (BU N) and serum creatinine were determined to evaluate renal function. Renal tissues were served to histological examination (PAS stain and immunofluorescence). The antibodies, including T GF-β (transforming growth factor-β), MMP-9 (matrix metalloproteinase-9), HGF (hepatocyte growth factor), were chosen to recognize the specific antigens in injury sites.
