白殭菌液態發酵產生胜類抗生素的研究 陳宴淑、張耀南、曾耀銘
E-mail: [email protected]
摘 要
白殭菌素已知為環狀胜?毒素之一，主要為白殭菌生合成產物。本研究以農業藥物毒物試驗所提供之白殭菌Beauveria bassiana A1為培養菌株，嘗試利用不同培養基進行液態發酵培養白殭菌生合成其代謝物-白殭菌素，初步結果顯示以25 g/L sucrose、25 mL/L edible molasses、10 g/L malt extract、10 g/L NZ broth、2 g/L K2HPO4為培養基質時，幾乎無白殭菌素 之產生，但若以玉米水萃取液為基礎培養基質，再添加上述培養基質濃度，基質起始調配pH值為6.2，在25 ℃下以150 rpm 振盪培養76 h後，可得到本實驗室目前最佳白殭菌素產量為699μg/L，由此可知玉米水萃取液為影響白殭菌生合成白殭菌 素的重要組成分之一。
關鍵詞 : 白殭菌、白殭菌素、玉米水萃取液
目錄
第一章 緒論 ...1 第二章 文獻回顧 ...4 2.1蟲生真菌的分類 ...4 2.2白殭菌屬分種 ...8 2.3白殭菌殺蟲原理
...10 2.4白殭菌之寄生範圍及標的害蟲 ...12 2.5微生物農藥 ...12 2.6白殭菌素 ...15 2.7農用抗生素 ...16 2.8胜?類抗生素 ...17 2.9 HPLC之儀器原理 ...19 第三章 材料與方法 ...21 3.1實驗材料 ...21 3.2儀器設備 ...21 3.3實驗方法
...22 3.3.1培養方法 ...22 3.3.2白殭菌素以HPLC定量分析 ...23 第四章 結果與討論 ...28 4.1培養基之最適化研究
...28 4.2最適玉米之測試 ...28 4.3最適玉米水萃取液之測試 ...29 第五章 結論與展望 ...33 參考文獻
...34 附錄 ...43 參考文獻
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