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紫杉醇誘發攝護腺癌細胞凋亡之機 制研究

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紫杉醇誘發攝護腺癌細胞凋亡之機 制研究

太平洋紫杉醇 (Paclitaxel, Taxol) 是一種有效的化療藥物用來治療多種 癌症,包括攝護腺癌。太平洋紫杉醇可顯著得誘發攝護腺癌細胞的死 亡與細胞凋亡經由細胞周期的停止於 G2/M , Bcl-2 的磷酸化,調降 Bcl-XL ,或增加 Bax 的基因表現。在這項研究中 , 我們研究可能的 機制如何太平洋紫杉醇誘發攝護腺癌細胞凋亡是否透過 p53 蛋白分子 去誘發粒線體中超氧化物陰離子自由基。當給予攝護腺癌細胞株 LNC aP 處理太平洋紫杉醇時,其誘發的細胞型態改變與細胞死亡具有時間 及濃度效應。而太平洋紫杉醇穩定微小管並導致細胞週期停滯於 G2/

M 階段,此部分的細胞死亡乃透過細胞凋亡形式進行。而同時於藥物 處理一小時內活化了 p53 蛋白活性,造成 p53 蛋白發生粒線體的轉位

,誘導粒線體中超氧化物自由基增加,接著粒線體凋亡路徑被啟動進 行,最終導致攝護腺癌細胞的計畫性死亡。這項研究的結果顯示太平 洋紫杉醇誘發 LNCaP 細胞凋亡可透過不同的機制,由較早 p53 蛋白 的粒線體轉位後,活氧物的生成,與最終粒線體凋亡路徑,或於細胞 週期停滯於 G2/M 而產生。

(2)

MECHANISMS OF PACLITAXEL-INDUCED APO PTOSIS OF PROSTATE CANCER CELLS

Paclitaxel, also known as Taxol, is an effective chemotherapeutic drug against a var

iety of cancers, including prostate cancer. Paclitaxel significantly induces cell death

and apoptosis of prostate cancer cells via G2/M cell cycle arrest, Bcl-2 phosphoryla

tion, down-regulation of Bcl-XL, up-regulation of Bax. In this study, we studied the

possible mechanism how Paclitaxel induced apoptotic insults to human prostate can

cer cells via p53-induced production of mitochondrial superoxide radicals. Adminis

tration of LNCaP, a human prostate cancer cell line, with Paclitaxel concentration-

and time-dependently induced morphological alteration and cell death. The cell dea

th was resulted from that Paclitaxel stabilized microtubules and caused cell cycle ar

rest in G2/M. The cell death partially resulted from apoptosis. Besides we also foun

d that p53 protein was activated. Then p53 translocated from cytoplasm to mitocho

ndria and induced the production of superoxide radicals. Therefore further mitocho

ndrial apoptotic pathway was proceeded and finally resulted in prostate cancer cell

progressive cell death. Results of this study show that Paclitaxel can induce apoptot

ic insults to LNCaP through alternative mechanism of earlier mitochondrial translo

cation of p53 which induced ROS production or later cell cycle G2/M arrest.

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